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ID   PKJB1      preliminary; circular DNA; SYN; 3442 BP.
XX
AC   IG9931;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKJB1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pUC8CM from pUC8 & cat gene
RC   pKJB1 from pUC8CM
RC   pKJB231.1 from pKJB1
RA   Buckley K.;
RT   ;
RL   Unpublished (1984).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pKJB1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pUC8)(Tn9)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 2665bp, TaqI = 248 998 2442
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pUC8CM 3438bp
FT                   1. pUC8CM EcoRI 3438bp 231..231
FT                   fill in
FT                   -> pKJB1 3442bp [no EcoRI]"
FT   -               1..234
FT                   /note="pUC8 1..234 234bp
FT                   EcoRI = G^AATT C
FT                   EcoRI =      G^AATTC"
FT   -               235..251
FT                   /note="pUC8 231..247 17bp
FT                   TaqI = T^CGA"
FT   -               252..1024
FT                   /note="Tn9 215..987 773bp
FT                   TaqI = T^CGA"
FT   -               1025..3442
FT                   /note="pUC8 248..2665 2418bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3442 BP; 867 A; 842 C; 850 G; 883 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattaattcc
     cggggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga
     attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
     gtgcccttaa acgcctggtg ctacgcctga ataagtgata ataagcggat gaatggcaga
     aattcgacct gcagccaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     cagtgagcga ggaagcggaa ga
//