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ID   PKK162     preliminary; circular DNA; SYN; 5800 BP.
XX
AC   IG0063;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKK16-2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK9-4 from pBR322 & pKK3535
RC   pKK16-2 from pKK9-4 & pKK3535
RC   pKK67-4 from pKK16-2
RC   pKK84-1 from pKK67-4 & pBR322
RC   pKK92c-2 from pKK84-1 & linker
RC   pKK161-8 from pKK92c-2 & M13mp8
RC   pKK175-6 from pKK161-8 & terminator T1
RC   pKK231-1 from pKK175-6 & pKK9-4 & pCM71
RC   pKK232-8 from pKK231-1 & pKK3535
RC   pKK122-3 from pKK92c-2 & pKK3535
RC   pKK100-17 from pKK92c-2 & pKK3535
RA   Brosius J.;
RT   "Plasmid vectors for the selection of promoters";
RL   Gene 27:151-160(1984).
XX
CC   The deletion/replacement results in the removal of the -35 region
CC   of the tet promoter (and so makes low levels of TET) and part of the
CC   amp gene of the pKK9-4.
CC   NM (pKK16-2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(E.coli DH1)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pKK9-4)(pKK353-5)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK3535 PstI-HindIII 1294bp 608..1902,
FT                   \ rrnB T1T2 term
FT                   \ PstI = 608 11047
FT                   \ HindIII = 1902 2470, rrnB T1T2 term
FT                   2. pKK9-4 PstI 5285bp 4536..4536
FT                   -> pKK16-2 5800bp"
FT   promoter        0..0
FT                   /note="PRO E. coli rrnB gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             56..1246
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             1396..1891
FT                   /note="GEN E. coli T1, T2, & 5S genes"
FT   CDS             1892..2388
FT                   /note="GEN E. coli T1, T2, & 5S genes"
XX
SQ   Sequence 5800 BP; 1334 A; 1572 C; 1561 G; 1333 T; 0 other;
     gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg
     ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat
     ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca
     ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc
     gactacgcga tcatggcgac cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg
     gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     aagggagagc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc
     attatgattc ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc
     aggcaggtag atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc
     ctaacttcga tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca
     tggaacgggt tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg
     cgtcgcggtg catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta
     acggattcac cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc
     aaaccaaccc ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc
     gcatcctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc
     agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac
     cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat
     gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg
     agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata
     aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct
     acaaactctt cctgtcgtca tatctacaag ccatcccccc acagatacgg taaactagcc
     tcgtttttgc atcaggaaag cctgttttgg cggatgagag aagattttca gctgatacag
     attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg
     gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt
     gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
     gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
     aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
     cctttttgcg tttctacaaa ctcttcctgt cgtcatatct acaagccatc cccccacaga
     tacggtaaac tagcctcgtt tttgcatcag gaaagcagtg ggcagcgttg ggtcctggcc
     acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
     tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
     aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
     ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
     ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
     gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
     taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
     tatcattacc cccatgaaca gaaattcccc cttacacgga ggcatcaagt gaccaaacag
     gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag
     aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac
     gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     ctgcaggacg ggaatacacc ttgtctggca gctacacctt ctgaaccacg tcccaccgtg
     ctggtgtttg actccggcgt cggtgggttg tcggtctatg acgagatccg gcatctctta
     ccggatctcc attacattta tgctttcgat aacgtcgctt tcccgtatgg cgaaaaaagc
     gaagcgttta ttgttgagcg agtggtggca attgtcaccg cggtgcaaga acgttatccc
     cttgcgctgg ctgtggtcgc ttgcaacact gccagtaccg tttcacttcc tgcattacgc
     gaaaagttcg acttcccggt tgttggtgtc gtgccggcga ttaaacctgc tgcacgtctg
     acggcaaatg gcattgtcgg attactggca acccgcggaa cagttaaacg ttcttatact
     catgagctga tcgcgcgttt cgctaatgaa tgccagatag aaatgctggg ctcggcagag
     atggttgagt tggctgaagc gaagctacat ggcgaagatg tttctctgga tgcactaaaa
     cgtatcctac gcccgtggtt aagaatgaaa gagccgccag ataccgttgt attgggttgc
     acccatttcc ctctactaca agaagaactg ttacaagtgc tgccagaggg aacccggctg
     gtggattctg gcgcagcgat tgctcgccga acggcctggt tgttagaaca tgaagccccg
     gatgcaaaat ctgccgatgc gaatattgcc ttttgtatgg caatgacgcc aggagctgaa
     caattattgc ccgttttaca gcgttacggc ttcgaaacgc tcgaaaaact ggcagtttta
     ggctgatttg gttgaatgtt gcgcggtcag aaaattattt taaatttcct cttgtcaggc
     cggaataact ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg
     gtcagcgggg ttctcctgag aactccggca gagaaagcaa aaataaatgc ttgactctgt
     agcgggaagg cgtattatgc acaccccgcg ccgctgagaa aaagcgaagc ggcactgctc
     tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
     agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg aagtttaatt
     ctttgagcgt caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg
     gcaggcctaa cacatgcaag tcgaacggta acaggaagaa
//