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ID   PKK1756    preliminary; circular DNA; SYN; 4398 BP.
XX
AC   IG0064;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKK175-6 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK9-4 from pBR322 & pKK3535
RC   pKK16-2 from pKK9-4 & pKK3535
RC   pKK67-4 from pKK16-2
RC   pKK84-1 from pKK67-4 & pBR322
RC   pKK92c-2 from pKK84-1 & linker
RC   pKK161-8 from pKK92c-2 & M13mp8
RC   pKK175-6 from pKK161-8 & terminator T1
RC   pKK231-1 from pKK175-6 & pKK9-4 & pCM71
RC   pKK232-8 from pKK231-1 & pKK3535
RC   pKK122-3 from pKK92c-2 & pKK3535
RC   pKK100-17 from pKK92c-2 & pKK3535
RA   Brosius J.;
RT   "Plasmid vectors for the selection of promoters";
RL   Gene 27:151-160(1984).
XX
CC   old Pharmacia vector.
CC   NM (pKK175-6)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(E.coli DH1)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pKK161-8)(E.coli rrnB)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK161-8 PvuII-PvuII 4150bp 2929..5260..1819
FT                   -> plasmid 4150bp
FT                   1. plasmid EcoRI 4150bp 5222..5222
FT                   2. E. coli EcoRI-EcoRI 168bp, ecoRGNB 6610-6778
FT                   \ rrnB terminator T1
FT                   -> pKK175-6 4398bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             23..1213
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             1363..1858
FT                   /note="GEN E. coli T1, T2, & 5S genes"
FT   terminator      >1363..<1858
FT                   /note="TER E. coli rrnB transcription terminator"
FT   CDS             complement(3115..3975)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 4398 BP; 999 A; 1201 C; 1173 G; 1025 T; 0 other;
     ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac
     cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg
     ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc
     gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg
     cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac
     acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc
     cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg
     ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc
     ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg
     tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt
     gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
     cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
     gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct
     gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt
     aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc
     cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt
     ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata
     tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tcctgttttg gcggatgaga
     gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa
     tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa
     acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc
     atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc
     aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc
     agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttcct gtcgtcatat
     ctacaagcca tccccccaca gatacggtaa actagcctcg tttttgcatc aggaaagcct
     gttttggcgg atgagagaag attttcagct gcctcgcgcg tttcggtgat gacggtgaaa
     acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa ggaattccag gcatcaaata
     aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc
     ggagggtggc gggcaggacg cccgccataa actgccagga attcccgggg atccgtcgac
     ctgcagccaa gcttcttg
//