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ID   PKK2233    preliminary; circular DNA; SYN; 4584 BP.
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AC   M77749; IG0335;
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DT   23-OCT-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKK223-3 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pKK125-1 from pKK92c-2 & pKK3535 & linker
RC   pKK176-2, pKK176-3 from pKK125-1 & linker
RC   pAH1-1 from pKK125-1 & pKK231-1
RC   pAH3-4 from pKK176-2 & pKK231-1
RC   pAH4-1 from pKK176-3 & pKK231-1
RC   pAH7-2 from pAH1-1 & linker
RC   pAH9-2 from pAH3-4 & linker
RC   pAH10-2 from pAH4-1 & linker
RC   pKK278-8 from pAH1-1 & pKK34-121 & pKK92c-2 & pKK231-1
RC   pKK279-1 from pAH3-4 & pKK34-121 & pKK92c-2 & pKK231-1
RC   pKK287-12 from pAH4-1 & pKK34-121 & pKK92c-2 & pKK231-1
RC   pKK223-3 from pKK10-2 & ptac11 & linker & pUC8
RA   Brosius J., Holy A.;
RT   "Regulation of ribosomal RNA promoters with a synthetic lac
RT   operator";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:6929-6933(1984).
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RN   [2]
RC   pLC29-47 from ColE1 & E.coli dehydroquinate synthase gene
RC   pJB14 from pLC29-47 & pKK223-3
RA   Frost J.W., Bender J.L., Kadonaga J.T., Knowles J.R.;
RT   "Dehydroquinate synthase from Escherichia coli: purification,
RT   cloning, and construction of overproducers of the enzyme";
RL   Biochemistry 23:4470-4475(1984).
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RN   [3]
RC   from pKK series, human alpha-tubulin expression
RA   Yaffe B.M., Levison B.S., Szasz J., Sternlicht H.;
RT   "Expression of a human alpha-tubulin: properties of the isolated
RT   subunit";
RL   Biochemistry 27:1869-1880(1988).
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RN   [4]
RC   from pKK223-3
RC   from pKK233-2
RA   Kozak M.;
RT   "Comparison of initiation of protein synthesis in Procaryotes,
RT   Eucaryotes, and organelles";
RL   Microbiological Reviews 47:1-45(1983).
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RN   [5]
RP   1-4586 (old)
RC   pKK223-3
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [6]
RC   pKK34- series from pKK34-121
RC   pKK35- series from pKK35-120
RA   Kingston R.E.;
RT   "Effects of deletions near Escherichia coli rrnB promoter P2 on
RT   inhibition of in vitro transcription by guanosine tetraphosphate";
RL   Biochemistry 22:5249-5254(1983).
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CC   GenBank entry is not current with Pharmacia entry (1993).
CC   NM (pKK223-3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli JM105)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pBR322)(pKK10-2)(ptacII)(pUC8)
CC   BR (pKK233-2)(pKK232-8)
CC   OF (pLC29-47)(pJB14)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   2. pKK3535
FT                   -> pKK5-1
FT                   1. pKK5-1
FT                   -> pKK8-18
FT                   1. pKK8-18
FT                   2. linker
FT                   -> pKK10-2
FT                   1. pKK10-2 large BamHI-HindIII 5339bp 2470..7809,
FT                   \ pKK3535 BamHI = 306 7809
FT                   \ pKK3535 HindIII = 1902 2470
FT                   2. ptac11 HindIII 4600bp
FT                   fill in
FT                   BamHI linker 10bp ccggatccgg:BamHI linker 10bp
FT                   \ ccggatccgg
FT                   BamHI-EcoRI 260bp, tac promoter
FT                   3. pUC8 EcoRI-HindIII 30bp 231..261, MCS
FT                   -> plasmid 5600bp
FT                   1. plasmid remove PvuI-BglI, amp gene/3300bp
FT                   2. pUC8 PvuI-BglI 1147bp 387..1534, amp gene/no PstI
FT                   -> pKK223-3 4584bp [unique PstI]"
FT   misc_binding    join(4552..4584,1..11)
FT                   /note="MCS unique HindIII-PstI-SalI-BamHI-SmaI-EcoRI"
FT   promoter        complement(11..>88)
FT                   /note="PRO E. coli tac (trp -35 and lacUV5 -10)"
FT   misc_binding    443..443
FT                   /note="SIT SphI"
FT   misc_binding    1945..1945
FT                   /note="SIT PvuII"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3613..3613
FT                   /note="SIT PvuI"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T1"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T2"
FT   CDS             0..0
FT                   /note="GEN E. coli 5S gene"
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SQ   Sequence 4584 BP; 1042 A; 1269 C; 1191 G; 1082 T; 0 other;
     ttctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacattata cgagccgatg
     attaattgtc aacagctcat ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa
     aaacattatc cagaacggga gtgcgccttg agcgacacga attatgcagt gatttacgac
     ctgcacagcc ataccacagc ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt
     gcagtcgata agcccggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
     caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
     acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
     gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
     tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga
     tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc
     ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca
     aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg
     tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt
     ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc
     atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac
     cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga
     ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc
     gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag
     aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa
     catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg
     gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg
     gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg
     ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc
     gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc
     aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg
     accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca
     acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg
     tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac
     caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct
     tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca
     cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa
     cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     gccattgcta cagcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaaagagt ttgtagaaac
     gcaaaaaggc catccgtcag gatggccttc tgcttaattt gatgcctggc agtttatggc
     gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc
     ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcatgggg
     agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca tggggtcagg
     tgggaccacc gcgctactgc cgccaggcaa attctgtttt atcagaccgc ttctgcgttc
     tgatttaatc tgtatcaggc tgaaaatctt ctctcatccg ccaaaacagc caagcttggc
     tgcaggtcga cggatccccg ggaa
//