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ID   PKK2311    preliminary; circular DNA; SYN; 5031 BP.
XX
AC   IG0065;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKK231-1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK9-4 from pBR322 & pKK3535
RC   pKK16-2 from pKK9-4 & pKK3535
RC   pKK67-4 from pKK16-2
RC   pKK84-1 from pKK67-4 & pBR322
RC   pKK92c-2 from pKK84-1 & linker
RC   pKK161-8 from pKK92c-2 & M13mp8
RC   pKK175-6 from pKK161-8 & terminator T1
RC   pKK231-1 from pKK175-6 & pKK9-4 & pCM71
RC   pKK232-8 from pKK231-1 & pKK3535
RC   pKK122-3 from pKK92c-2 & pKK3535
RC   pKK100-17 from pKK92c-2 & pKK3535
RA   Brosius J.;
RT   "Plasmid vectors for the selection of promoters";
RL   Gene 27:151-160(1984).
XX
CC   NM (pKK231-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(E.coli DH1)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pKK175-6)(pCM71)(pKK9-4)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK175-6 NaeI-HindIII 3167bp 1223..4390
FT                   \ NaeI = 341 709 869 1223
FT                   2. pCM71 HindIII-SalI 725bp 3115..3370..470, cat gene
FT                   :fill in
FT                   -> plasmid 3892bp
FT                   1. plasmid PvuII 3892bp, pKK175-6 1889..1889
FT                   2. pKK9-4 PvuII-PvuII 1139bp 1852..2991
FT                   -> pKK231-1 5031bp"
FT   misc_feature    0..0
FT                   /note="insert 1100 bp PvuII fragment of pKK9-4
FT                   into intermediate cut with PvuII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             140..635
FT                   /note="GEN E. coli T1, T2, & 5S genes"
FT   CDS             666..1163
FT                   /note="GEN E. coli T1, T2, & 5S genes"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB transcription terminator"
FT   CDS             complement(3031..3891)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             complement(4314..5100)
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr),
FT                   from pCM71"
XX
SQ   Sequence 5031 BP; 1276 A; 1265 C; 1275 G; 1215 T; 0 other;
     gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg
     cggcgcatcc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc
     cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt
     ttctacaaac tcttcctgtc gtcatatcta caagccatcc ccccacagat acggtaaact
     agcctcgttt ttgcatcagg aaagcctgtt ttggcggatg agagaagatt ttcagctgtt
     ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc
     tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga
     actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag
     ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     cctgtcgtca tatctacaag ccatcccccc acagatacgg taaactagcc tcgtttttgc
     atcaggaaag caggggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
     cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
     ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
     ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
     ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
     cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatt
     cccccttaca cggaggcatc aagtgaccaa acaggaaaaa accgccctta acatggcccg
     ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga
     acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct
     cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     cttcaaggaa ttccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
     atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
     ccaggaattc ccggggatcc gtcgacctgc agccaagctt cgacgagatt ttcaggagct
     aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     ccggcctttg aattcacatt cttgcccgcc tgatgaatgc tcatccggaa attctcatga
     ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct
     ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg t
//