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ID   PKK2328    preliminary; circular DNA; SYN; 5094 BP.
XX
AC   IG0066;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKK232-8 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK9-4 from pBR322 & pKK3535
RC   pKK16-2 from pKK9-4 & pKK3535
RC   pKK67-4 from pKK16-2
RC   pKK84-1 from pKK67-4 & pBR322
RC   pKK92c-2 from pKK84-1 & linker
RC   pKK161-8 from pKK92c-2 & M13mp8
RC   pKK175-6 from pKK161-8 & terminator T1
RC   pKK231-1 from pKK175-6 & pKK9-4 & pCM71
RC   pKK232-8 from pKK231-1 & pKK3535
RC   pKK122-3 from pKK92c-2 & pKK3535
RC   pKK100-17 from pKK92c-2 & pKK3535
RA   Brosius J.;
RT   "Plasmid vectors for the selection of promoters";
RL   Gene 27:151-160(1984).
XX
CC   NM (pKK232-8)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli HB101)(E.coli DH1)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pBR322)(pKK9-4)(pKK353-5)(pCM71)(E.coli rrnB)
CC   BR (pKK223-3)(pKK233-2)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK3535 DdeI-HpaII 20bp 6689..6708, rrnB
FT                   fill in:fill in
FT                   2. pKK231-1 HindIII 5031bp 4236..4236
FT                   fill in
FT                   -> pKK232-8 5094bp"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T1"
FT   misc_binding    177..177
FT                   /note="SIT double EcoRI"
FT   misc_binding    177..212
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII
FT                   from M13mp8"
FT   misc_binding    >177..<212
FT                   /note="SIT unique SmaI"
FT   misc_binding    >177..<212
FT                   /note="SIT unique BamHI"
FT   misc_binding    >177..<212
FT                   /note="SIT unique SalI"
FT   misc_binding    >177..<212
FT                   /note="SIT unique HindIII"
FT   terminator      >212..<380
FT                   /note="TER stop codons in all 3 translation frames
FT                   from pKK3535"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr),
FT                   from pCM71"
FT   CDS             0..0
FT                   /note="GEN E. coli 5S genes, from pKK3535"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T1, from pKK3535"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T2, from pKK3535"
FT   misc_binding    1658..1658
FT                   /note="SIT PvuII"
FT   CDS             0..0
FT                   /note="GEN E. coli 5S genes, from pKK9-4"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T1, from pKK9-4"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T2, from pKK9-4"
FT   misc_binding    2797..2797
FT                   /note="SIT PvuII"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    5092..5092
FT                   /note="SIT double EcoRI"
XX
SQ   Sequence 5094 BP; 1293 A; 1290 C; 1290 G; 1221 T; 0 other;
     ttcccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccagggaat
     tcccggggat ccgtcgacct gcagccaagc ttgagtagga caaatccgcc gagcttcgac
     gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
     tgatatatcc caatcgcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
     gcctggtgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgtcgagg
     cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga
     actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc
     agccgcacgc ggcgcatctc ggctgttttg gcggatgaga gaagattttc agcctgatac
     agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
     cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     ggcctttttg cgtttctaca aactcttcct gtcgtcatat ctacaagcca tccccccaca
     gatacggtaa actagcctcg tttttgcatc aggaaagcag ctgttttggc ggatgagaga
     agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
     tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
     gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
     caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
     cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
     aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgt cgtcatatct
     acaagccatc cccccacaga tacggtaaac tagcctcgtt tttgcatcag gaaagcagtc
     gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
     gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
     gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
     tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg
     gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa
     gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg
     tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag
     catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga
     ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
     gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg
     tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga
     tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa
//