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ID   PKK2332    preliminary; circular DNA; SYN; 4593 BP.
XX
AC   X70478;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKK233-2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCI15, pcI16 from pEA305 & pKK233-2
RC   [pKK188-21 from ptac12]
RC   pKK207-1 from ptac12 & linker
RC   pKK216-1 from pKK207-1 & linker
RC   pKK240-11 from pKK216-1 & pKK10-2
RC   pKK217-1 from pKK188-21 & linker
RC   pKK218-1 from pKK217-1
RC   pKK233-2 from pKK218-1 & linker & pKK223-3
RC   pMF105 from pKK240-11
RC   pMF106 from pMF105 & pKK240-11
RC   pUC12-STOP from pUC12
RC   pMF107 from pMF106 & pUC12-STOP
RA   Amann E., Brosius J.;
RT   "ATG vectors for regulated high-level expression of cloned genes
RT   in Escherichia coli";
RL   Gene 40:183-190(1985).
XX
RN   [2]
RC   pcT7 from pBR322 & chicken TIM gene
RC   pgT7, pgT9 from Charon 4A & chicken genome
RC   pTF2 from pcT7 & pgT9
RC   pX0 from pTF2 & pKK233-2
RC   pX1 from pX0 & pKK233-2
RA   Straus D., Gilbert W.;
RT   "Chicken triosephosphate isomerase complements an Escherichia coli
RT   deficiency";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:2014-2018(1985).
XX
RN   [3]
RC   pCL1 from pBR322 & rat LCI
RC   pELPI-II from pKK233-2 & rat LCI
RA   Shimizu Y., Takabayashi E., Yano S.Y., Shimizu N., Yamada K.,
RA   Gushima H.;
RT   "Molecular cloning and expression in Escherichia coli of the cDNA
RT   coding for rat lipocortin I (calpactin II)";
RL   Gene 65:141-148(1988).
XX
RN   [4]
RC   from pKK223-3
RC   from pKK233-2
RA   Kozak M.;
RT   "Comparison of initiation of protein synthesis in Procaryotes,
RT   Eucaryotes, and organelles";
RL   Microbiological Reviews 47:1-45(1983).
XX
RN   [5]
RP   1-4593
RC   pKK233-2
RA   Thomas C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Thomas C., Dept. of Microbiology and Immunology,
RL   University of Adelaide, Australia.
XX
CC   Invitrogen sequence is 4523 bp & is the inverse-complement of this
CC   sequence. It is missing the first 2 bases gg and starts at base 3.
CC   It is missing aa after base 373. It has an extra t after base 3054.
CC   It has an extra a after base 3075. It is missing the bases after 4523.
CC   NM (pKK233-2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)(Pharmacia)
CC   HO (E.coli JM109)(E.coli W3110-LacIQ)(E.coli RB791)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. ptac12 2600bp
FT                   -> pKK188-21
FT                   1. pKK188-21 EcoRI, pBR322 4360..4360
FT                   fill in
FT                   BamHI linker 6bp ggatcc
FT                   -> pKK217-1 [EcoRI]
FT                   1. pKK217-1 PvuII, pBR322  2069..2069
FT                   fill in
FT                   NcoI linker 8bp accatggt
FT                   -> pKK218-1
FT                   1. pKK218-1 NcoI
FT                   fill in
FT                   PstI linker 6bp ctgcag
FT                   PstI-BamHI, promoter
FT                   2. pKK223-3 remove PstI-BamHI 8bp 4565..4573, 4576bp
FT                   -> pKK223-2 4593bp"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2923..3783)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   CDS             3860..4298
FT                   /note="GEN E. coli T1, T2, & 5S genes"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T1"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T2"
FT   CDS             0..0
FT                   /note="GEN E. coli 5S gene"
FT   misc_feature    0..0
FT                   /note="ATG in an NcoI site"
FT   RBS             0..0
FT                   /note="RBS E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-NcoI"
FT   promoter        complement(4322..4395)
FT                   /note="PRO E. coli trc (trp -35 and lacUV5 -10)"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
XX
SQ   Sequence 4593 BP; 1049 A; 1268 C; 1190 G; 1086 T; 0 other;
     ccggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgccgttgc
     tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc
     cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
     cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
     aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
     gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac
     ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
     cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat
     caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg
     tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg
     gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
     ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
     tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
     acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
     ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
     tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
     cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
     attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa
     aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
     tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
     atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
     acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     atacatattt gaatgtattt agaaaaataa acaaaaagag tttgtagaaa cgcaaaaagg
     ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct
     gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc
     ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg gagaccccac
     actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac
     cgcgctactg ccgccaggca aactgtttta tcagaccgct tctgcgttct gatttaatct
     gtatcaggct gaaaatcttc tctcatccgc caaaacagcc aagcttggct gcagccatgg
     tctgtttcct gtgtgaaatt gttatccgct cacaattcca cacattatac gagccggatg
     attaattgtc aacagctcat ttcagaatat ttgccagaac cgtttatatg tcggcgcaaa
     aaacattatc cagaacggga gtgcgccttg agcgacacga attatgcagt gatttacgac
     ctgcacagcc aatccacagc ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt
     gcagtcgatg ataagctgtc aaacatgaga att
//