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ID   PKK3535    preliminary; circular DNA; SYN; 11796 BP.
XX
AC   IG0068;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKK353-5 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK115 from pTUB2 & pBR322
RC   pKK123 from pTUB2 & pBR313
RC   pKK2361 from pBR313 & lambda rifd18, rrnB cistron
RC   pKK3535 from pKK2361 & pBR322
RA   Brosius J., Ullrich A., Raker M.A., Gray A., Dull T.J., Gutell R.R.,
RA   Noller H.F.;
RT   "Construction and fine mapping of recombinant plasmids containing
RT   the rrnB ribosomal RNA operon of E. coli";
RL   Plasmid 6:112-118(1981).
XX
RN   [2]
RC   lambda rifd18 from tufB gene promoter & lacZ gene
RC   pTUB1 from RSF2124 & lambda rifd18, tufB gene promoter/lacZ gene
RC   pTUB2 from RSF2124 & lambda rifd18, tufB gene promoter/lacZ gene
RA   Miyajima A., Shibuya M., Kaziro Y.;
RT   "Construction and characterization of the two hybrid ColE1 plasmids
RT   carrying Escherichia coli tufB gene";
RL   FEBS Lett. 102:207-210(1979).
XX
RN   [3]
RC   pER18 from ColE1 & lambda rifd18, rrnB cistron
RC   pER24 from ColE1 & lambda rifd18, rrnB cistron
RC   pKK115 from pBR322 & pER24
RA   Palmer M.L., Raker M.A., Kennedy P.J., Young J.W., Barnes W.M.,
RA   Rodriguez R.L., Noller H.F.;
RT   "Isolation and restriction mapping of plasmids containing ribosomal
RT   DNA sequences from the rrnB cistron of E. coli";
RL   Mol. Gen. Genet. 172:171-178(1979).
XX
RN   [4]
RC   pTUA1 from RSF2124 & lambda fus3, tufA gene
RA   Shibuya M., Nashimoto H., Kaziro Y.;
RT   "Cloning of an EcoRI fragment carrying E. coli tufA gene";
RL   Mol. Gen. Genet. 170:231-234(1979).
XX
CC   NM (pKK353-5)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(E.coli DH1)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda 48502bp
FT                   2. E. coli tufB gene promoter
FT                   3. E. coli lacZ gene
FT                   -> lambda rifd18 49000bp
FT                   1. pBR313 BamHI 5800bp
FT                   2. lambda rifd18 BamHI-BamHI 7500bp, E. coli rrnB
FT                   -> pKK2361 13300bp
FT                   1. pBR322 BamHI 4361bp 376..376, tet gene
FT                   2. pKK2361 BamHI-BamHI 7500bp, rrnB operon
FT                   -> pKK353-5 11796bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             6790..7287
FT                   /note="GEN E. coli T1, T2, & 5S genes"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB transcription terminator"
FT   CDS             complement(10798..11658)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 11796 BP; 2933 A; 2889 C; 3287 G; 2687 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc gcctaccttt cacgagttgc gcagtttgtc tgcaagactc tatgagaagc
     agataagcga taagtttgct caacatcttc tcgggcataa gtcggacacc atggcatcac
     agtatcgtga tgacagaggc agggagtggg acaaaattga aatcaaataa tgattttatt
     ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttt ttataatgcc
     aacttagtat aaaatagcca acctgttcga caaagattat gagacagtct atggctacca
     aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca cgtcccaccg
     tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc cggcatctct
     taccggatct ccattacatt tatgctttcg ataacgtcgc tttcccgtat ggcgaaaaaa
     gcgaagcgtt tattgttgag cgagtggtgg caattgtcac cgcggtgcaa gaacgttatc
     cccttgcgct ggctgtggtc gcttgcaaca ctgccagtac cgtttcactt cctgcattac
     gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct gctgcacgtc
     tgacggcaaa tggcattgtc ggattactgg caacccgcgg aacagttaaa cgttcttata
     ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg ggctcggcag
     agatggttga gttggctgaa gcgaagctac atggcgaaga tgtttctctg gatgcactaa
     aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt gtattgggtt
     gcacccattt ccctctacta caagaagaac tgttacaagt gctgccagag ggaacccggc
     tggtggattc tggcgcagcg attgctcgcc gaacggcctg gttgttagaa catgaagccc
     cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg ccaggagctg
     aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt
     taggctgatt tggttgaatg ttgcgcggtc agaaaattat tttaaatttc ctcttgtcag
     gccggaataa ctccctataa tgcgccacca ctgacacgga acaacggcaa acacgccgcc
     gggtcagcgg ggttctcctg agaactccgg cagagaaagc aaaaataaat gcttgactct
     gtagcgggaa ggcgtattat gcacaccccg cgccgctgag aaaaagcgaa gcggcactgc
     tctttaacaa tttatcagac aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg
     caagacgaaa aatgaatacc aagtctcaag agtgaacacg taattcatta cgaagtttaa
     ttctttgagc gtcaaacttt taaattgaag agtttgatca tggctcagat tgaacgctgg
     cggcaggcct aacacatgca agtcgaacgg taacaggaag aagcttgctt ctttgctgac
     gagtggcgga cgggtgagta atgtctggga aactgcctga tggaggggga taactactgg
     aaacggtagc taataccgca taacgtcgca agaccaaaga gggggacctt cgggcctctt
     gccatcggat gtgcccagat gggattagct agtaggtggg gtaacggctc acctaggcga
     cgatccctag ctggtctgag aggatgacca gccacactgg aactgagaca cggtccagac
     tcctacggga ggcagcagtg gggaatattg cacaatgggc gcaagcctga tgcagccatg
     ccgcgtgtat gaagaaggcc ttcgggttgt aaagtacttt cagcggggag gaagggagta
     aagttaatac ctttgctcat tgacgttacc cgcagaagaa gcaccggcta actccgtgcc
     agcagccgcg gtaatacgga gggtgcaagc gttaatcgga attactgggc gtaaagcgca
     cgcaggcggt ttgttaagtc agatgtgaaa tccccgggct caacctggga actgcatctg
     atactggcaa gcttgagtct cgtagagggg ggtagaattc caggtgtagc ggtgaaatgc
     gtagagatct ggaggaatac cggtggcgaa ggcggccccc tggacgaaga ctgacgctca
     ggtgcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga
     tgtcgacttg gaggttgtgc ccttgaggcg tggcttccgg agctaacgcg ttaagtcgac
     cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg cccgcacaag
     cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt cttgacatcc
     acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt gctgcatggc
     tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
     ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
     aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
     tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
     gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
     agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
     tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
     atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
     ttaccttaaa gaagcgtact ttgtagtgct cacacagatt gtctgataga aagtgaaaag
     caaggcgttt acgcgttggg agtgaggctg aagagaataa ggccgttcgc tttctattaa
     tgaaagctca ccctacacga aaatatcacg caacgcgtga taagcaattt tcgtgtcccc
     ttcgtctaga ggcccaggac accgcccttt cacggcggta acaggggttc gaatccccta
     ggggacgcca cttgctggtt tgtgagtgaa agtcgccgac cttaatatct caaaactcat
     cttcgggtga tgtttgagat ttttgctctt taaaaatctg gatcaagctg aaaattgaaa
     cactgaacaa cgagagttgt tcgtgagtct ctcaaatttt cgcaacacga tgatgaatcg
     aaagaaacat cttcgggttg tgaggttaag cgactaagcg tacacggtgg atgccctggc
     agtcagaggc gatgaaggac gtgctaatct gcgataagcg tcggtaaggt gatatgaacc
     gttataaccg gcgatttccg aatggggaaa cccagtgtgt ttcgacacac tatcattaac
     tgaatccata ggttaatgag gcgaaccggg ggaactgaaa catctaagta ccccgaggaa
     aagaaatcaa ccgagattcc cccagtagcg gcgagcgaac ggggagcagc ccagagcctg
     aatcagtgtg tgtgttagtg gaagcgtctg gaaaggcgcg cgatacaggg tgacagcccc
     gtacacaaaa atgcacatgc tgtgagctcg atgagtaggg cgggacacgt ggtatcctgt
     ctgaatatgg ggggaccatc ctccaaggct aaatactcct gactgaccga tagtgaacca
     gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga gtgaaaaaga acctgaaacc
     gtgtacgtac aagcagtggg agcacgctta ggcgtgtgac tgcgtacctt ttgtataatg
     ggtcagcgac ttatattctg tagcaaggtt aaccgaatag gggagccgaa gggaaaccga
     gtcttaactg ggcgttaagt tgcagggtat agacccgaaa cccggtgatc tagccatggg
     caggttgaag gttgggtaac actaactgga ggaccgaacc gactaatgtt gaaaaattag
     cggatgactt gtggctgggg gtgaaaggcc aatcaaaccg ggagatagct ggttctcccc
     gaaagctatt taggtagcgc ctcgtgaatt catctccggg ggtagagcac tgtttcggca
     agggggtcat cccgacttac caacccgatg caaactgcga ataccggaga atgttatcac
     gggagacaca cggcgggtgc taacgtccgt cgtgaagagg gaaacaaccc agaccgccag
     ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg gaaggcccag acagccagga
     tgttggctta gaagcagcca tcatttaaag aaagcgtaat agctcactgg tcgagtcggc
     ctgcgcggaa gatgtaacgg ggctaaacca tgcaccgaag ctgcggcagc gacgcttatg
     cgttgttggg taggggagcg ttctgtaagc ctgcgaaggt gtgctgtgag gcatgctgga
     ggtatcagaa gtgcgaatgc tgacataagt aacgataaag cgggtgaaaa gcccgctcgc
     cggaagacca agggttcctg tccaacgtta atcggggcag ggtgagtcga cccctaaggc
     gaggccgaaa ggcgtagtcg atgggaaaca ggttaatatt cctgtacttg gtgttactgc
     gaagggggga cggagaaggc tatgttggcc gggcgacggt tgtcccggtt taagcgtgta
     ggctggtttt ccaggcaaat ccggaaaatc aaggctgagg cgtgatgacg aggcactacg
     gtgctgaagc aacaaatgcc ctgcttccag gaaaagcctc taagcatcag gtaacatcaa
     atcgtacccc aaaccgacac aggtggtcag gtagagaata ccaaggcgct tgagagaact
     cgggtgaagg aactaggcaa aatggtgccg taacttcggg agaaggcacg ctgatatgta
     ggtgaggtcc ctcgcggatg gagctgaaat cagtcgaaga taccagctgg ctgcaactgt
     ttattaaaaa cacagcactg tgcaaacacg aaagtggacg tatacggtgt gacgcctgcc
     cggtgccgga aggttaattg atggggttag cgcaagcgaa gctcttgatc gaagccccgg
     taaacggcgg ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc
     cgacctgcac gaatggcgta atgatggcca ggctgtctcc acccgagact cagtgaaatt
     gaactcgctg tgaagatgca gtgtacccgc ggcaagacgg aaagaccccg tgaaccttta
     ctatagcttg acactgaaca ttgagccttg atgtgtagga taggtgggag gctttgaagt
     gtggacgcca gtctgcatgg agccgacctt gaaataccac cctttaatgt ttgatgttct
     aacgttgacc cgtaatccgg gttgcggaca gtgtctggtg ggtagtttga ctggggcggt
     ctcctcctaa agagtaacgg aggagcacga aggttggcta atcctggtcg gacatcagga
     ggttagtgca atggcataag ccagcttgac tgcgagcgtg acggcgcgag caggtgcgaa
     agcaggtcat agtgatccgg tggttctgaa tggaagggcc atcgctcaac ggataaaagg
     tactccgggg ataacaggct gataccgccc aagagttcat atcgacggcg gtgtttggca
     cctcgatgtc ggctcatcac atcctggggc tgaagtaggt cccaagggta tggctgttcg
     ccatttaaag tggtacgcga gctgggttta gaacgtcgtg agacagttcg gtccctatct
     gccgtgggcg ctggagaact gaggggggct gctcctagta cgagaggacc ggagtggacg
     catcactggt gttcgggttg tcatgccaat ggcactgccc ggtagctaaa tgcggaagag
     ataagtgctg aaagcatcta agcacgaaac ttgccccgag atgagttctc cctgaccctt
     taagggtcct gaaggaacgt tgaagacgac gacgttgata ggccgggtgt gtaagcgcag
     cgatgcgttg agctaaccgg tactaatgaa ccgtgaggct taaccttaca acgccgaagc
     tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
     ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
     ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
     gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga
     tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
     caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
     tcttcctgtc gtcatatcta caagccatcc ccccacagat acggtaaact agcctcgttt
     ttgcatcagg aaagcagcta tgaaccactc cttaaaaccc tggaacacat ttggcattga
     tcataatgct cagcacattg tatgtgccga agacgaacaa caattactca atgcctggca
     gtatgcaacc gcagaaggac aacccgttct tattctgggt gaaggaagta atgtactttt
     tctggaggac tatcgcggca cggtgatcat caaccggatc aaaggtatcg aaattcatga
     tgaacctgat gcgtggtatt tacatgtagg agccggagaa aactggcatc gtctggtaaa
     atacactttg caggaaggta tgcctggtct ggaaaatctg gcattaattc ctggttgtgt
     cggctcatca cctatccaga atattggtgc ttatggcgta gaattacagc gagtttgcgc
     ttatgttgat tctgttgaac tggcgacagg caagcaagtg cgcttaactg ccaaagagtg
     ccgttttggc tatcgcgaca gtatttttaa acatgaatac caggaccgct tcgctattgt
     agccgtaggt ctgcgtctgc caaaagagtg gcaacctgta ctaacgtatg gtgacttaac
     tcgtctggga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc gatgcccttg
     agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc
     attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta
     ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc
     ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg
     gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc
     gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
     gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
     tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag
     ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca
     tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc
     cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
     ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
     aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
     tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
     gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag
     tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca
     gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg
     gtatcattac ccccatgaac agaaattccc ccttacacgg aggcatcaag tgaccaaaca
     ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga
     gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca
     cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     aataggcgta tcacgaggcc ctttcgtctt caagaa
//