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ID   PKK3881    preliminary; circular DNA; SYN; 5075 BP.
XX
AC   IG1083;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKK388-1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK388-1 from pBR322
RA   Rodriguez R.L., Denhardt D.T.;
RT   ;
RL   Vectors 0:213-221(1988).
RL   Rodriguez R.L., Denhardt D.T., eds., Butterworths, Boston.
XX
CC   NM (pKK388-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp/tet
FT                   2. E. coli, 5S gene/rrnB gene T1T2 terminators
FT                   3. E. coli, trc/lac promoters
FT                   -> pKK388-1 5075bp"
FT   CDS             complement(2923..3783)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T1"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T2"
FT   CDS             0..0
FT                   /note="GEN E. coli 5S gene"
FT   misc_feature    0..0
FT                   /note="ATG in an NcoI site"
FT   RBS             0..0
FT                   /note="RBS E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-
FT                   SmaI-KpnI-SacI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT NcoI"
FT   RBS             0..0
FT                   /note="RBS"
FT   misc_feature    0..0
FT                   /note="TER E. coli rrnB antitermination"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli trc (trp -35 and lacUV5 -10)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             complement(2923..3783)
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5075 BP; 1210 A; 1311 C; 1418 G; 1136 T; 0 other;
     ggccaatgct tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta
     aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg
     ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatccg
     gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagcgccgct
     gagaaaaagc gaagcggcac tgctctttaa caatttatca gacaatctgt gtgggcactc
     gaccggaatt atcgataagg aggtttaaac catggcagct gaattcgagc tcggtacccg
     gggatcctct agagtcgacc tgcaggcatg caagcttggc tgttttggcg gatgagagaa
     gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt
     gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg
     ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc
     aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac
     ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga
     aggccatcct gacggatggc ctttttgcgt ttctacaaac tctttttgtt tatttttcta
     aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
     tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt
     gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
     gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
     agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg
     ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca
     gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc
     cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga
     ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg
     aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag
     ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg
     cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca
     tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg
     cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag
     cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc
     gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca
     tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg
     agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct
     gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
     cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
     cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
     cggcgtagag gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc
     caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa
     cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg
     cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc
     ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt
     tcatacacgg tgcctgactg cgttagccca agctattccg cgcattatac gagccggaag
     cataaagtgt aaagcttgtg caggtcgacg gatcc
//