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ID   PKK4211    preliminary; circular DNA; SYN; 3542 BP.
XX
AC   IG9947;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKK421-1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK421-1 from pKK233-2
RC   pSLD7, pSLE1, pSLF1, pSLJ10 from pBluescript KS+
RC   pSL180 from pUC18 & linker
RC   pSL189 from pUC19 & linker
RC   pSL190 from pSL189
RC   pSL1180 from pUC118 & linker
RC   pSL1190 from pUC119 & linker
RC   pSL300 from pSLJ10 & linker
RC   pSL250 from pSL300
RC   pSL260 from pSL250
RC   pSL270 from pSL250
RC   pSL251 from pSL250
RC   pSL261 from pSL260
RC   pSL271 from pSL270
RC   pSL301 from pSL300
RC   pSE1200 from pUC120 & linker
RC   pSE1201 from pUC120 & linker
RC   pSE1202 from pUC120 & linker
RC   pSE219 from pKK233-2
RC   pSE220 from pSE219
RC   pSE280 from pKK421-1
RC   pSE380 from pTrc99A
RA   Brosius J.;
RT   "Superpolylinkers in cloning and expression vectors";
RL   DNA 8:759-777(1989).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pKK421-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli NM522)(E.coli INValphaF')
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pKK233-2)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK233-2 remove EcoRI-AvaI 1055bp
FT                   \ 4592..4593..1054, tet gene/3538bp
FT                   fill in:fill in
FT                   -> pKK421-1 3538bp"
FT   -               1..3542
FT                   /note="pKK233-2 1054..4593..2 3542bp
FT                   EcoRI = G^AATT C
FT                   AvaI =       C^YCGRG"
FT   promoter        0..0
FT                   /note="PRO E. coli trc (trp & lac)"
FT   RBS             0..0
FT                   /note="RBS"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   terminator      0..0
FT                   /note="TER"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 3542 BP; 870 A; 932 C; 875 G; 865 T; 0 other;
     tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
     cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
     aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
     tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg
     agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaattc ccccttacac
     ggaggcatca agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa
     gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca
     tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg
     gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     aaaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttaatttga
     tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcgcaacg
     ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag
     ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt
     ccctactctc gcatggggag accccacact accatcggcg ctacggcgtt tcacttctga
     gttcggcatg gggtcaggtg ggaccaccgc gctactgccg ccaggcaaac tgttttatca
     gaccgcttct gcgttctgat ttaatctgta tcaggctgaa aatcttctct catccgccaa
     aacagccaag cttggctgca gccatggtct gtttcctgtg tgaaattgtt atccgctcac
     aattccacac attatacgag ccggatgatt aattgtcaac agctcatttc agaatatttg
     ccagaaccgt ttatatgtcg gcgcaaaaaa cattatccag aacgggagtg cgccttgagc
     gacacgaatt atgcagtgat ttacgacctg cacagccaat ccacagcttc cgatggctgc
     ctgacgccag aagcattggt gcaccgtgca gtcgatgata agctgtcaaa catgagaatt
     cc
//