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ID   PKK674     preliminary; circular DNA; SYN; 5731 BP.
XX
AC   IG0069;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKK67-4 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK9-4 from pBR322 & pKK3535
RC   pKK16-2 from pKK9-4 & pKK3535
RC   pKK67-4 from pKK16-2
RC   pKK84-1 from pKK67-4 & pBR322
RC   pKK92c-2 from pKK84-1 & linker
RC   pKK161-8 from pKK92c-2 & M13mp8
RC   pKK175-6 from pKK161-8 & terminator T1
RC   pKK231-1 from pKK175-6 & pKK9-4 & pCM71
RC   pKK232-8 from pKK231-1 & pKK3535
RC   pKK122-3 from pKK92c-2 & pKK3535
RC   pKK100-17 from pKK92c-2 & pKK3535
RA   Brosius J.;
RT   "Plasmid vectors for the selection of promoters";
RL   Gene 27:151-160(1984).
XX
CC   NM (pKK67-4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(E.coli DH1)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pKK16-2)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK16-2 5800bp
FT                   BAL31 nuclease [45 bp from 16S;to pBR322 base 64]
FT                   HindIII linker 6bp aagctt
FT                   -> pKK67-4 5731bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             23..1213
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             1363..1858
FT                   /note="GEN E. coli T1, T2, & 5S genes"
FT   CDS             1859..2355
FT                   /note="GEN E. coli T1, T2, & 5S genes"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB transcription terminator"
XX
SQ   Sequence 5731 BP; 1316 A; 1550 C; 1543 G; 1322 T; 0 other;
     gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc
     cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa
     tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg
     ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg
     tggatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc
     ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg
     gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc
     ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc
     tgaatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc
     aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt
     ccgccatctc cagcagccgc acgcggcgca tcctgttttg gcggatgaga gaagattttc
     agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     cctgacggat ggcctttttg cgtttctaca aactcttcct gtcgtcatat ctacaagcca
     tccccccaca gatacggtaa actagcctcg tttttgcatc aggaaagcct gttttggcgg
     atgagagaag attttcagct gatacagatt aaatcagaac gcagaagcgg tctgataaaa
     cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa
     gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc
     caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc
     gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat
     taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc ttcctgtcgt
     catatctaca agccatcccc ccacagatac ggtaaactag cctcgttttt gcatcaggaa
     agcagcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag
     gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga
     gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt
     cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat
     gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt
     aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc
     cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat
     cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa attccccctt
     acacggaggc atcaagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
     agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca
     gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
     ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     atagtttgcg caacgttgtt gccattgctg caggacggga atacaccttg tctggcagct
     acaccttctg aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg
     gtctatgacg agatccggca tctcttaccg gatctccatt acatttatgc tttcgataac
     gtcgctttcc cgtatggcga aaaaagcgaa gcgtttattg ttgagcgagt ggtggcaatt
     gtcaccgcgg tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg caacactgcc
     agtaccgttt cacttcctgc attacgcgaa aagttcgact tcccggttgt tggtgtcgtg
     ccggcgatta aacctgctgc acgtctgacg gcaaatggca ttgtcggatt actggcaacc
     cgcggaacag ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc taatgaatgc
     cagatagaaa tgctgggctc ggcagagatg gttgagttgg ctgaagcgaa gctacatggc
     gaagatgttt ctctggatgc actaaaacgt atcctacgcc cgtggttaag aatgaaagag
     ccgccagata ccgttgtatt gggttgcacc catttccctc tactacaaga agaactgtta
     caagtgctgc cagagggaac ccggctggtg gattctggcg cagcgattgc tcgccgaacg
     gcctggttgt tagaacatga agccccggat gcaaaatctg ccgatgcgaa tattgccttt
     tgtatggcaa tgacgccagg agctgaacaa ttattgcccg ttttacagcg ttacggcttc
     gaaacgctcg aaaaactggc agttttaggc tgatttggtt gaatgttgcg cggtcagaaa
     attattttaa atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac
     acggaacaac ggcaaacacg ccgccgggtc agcggggttc tcctgagaac tccggcagag
     aaagcaaaaa taaatgcttg actctgtagc gggaaggcgt attatgcaca ccccgcgccg
     ctgagaaaaa gcgaagcggc actgctcttt aacaatttat cagacaatct gtgtgggcac
     tcgaagatac ggattcttaa cgtcgcaaga cgaaaaatga ataccaagtc tcaagagtga
     acacgtaatt cattacgaag tttaattctt tgagcgtcaa acttttaaat tgaagagttt
     gatcatggct cagattgaac gccaagcttg g
//