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ID   PKK94      preliminary; circular DNA; SYN; 5285 BP.
XX
AC   IG0072;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKK9-4 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK9-4 from pBR322 & pKK3535
RC   pKK16-2 from pKK9-4 & pKK3535
RC   pKK67-4 from pKK16-2
RC   pKK84-1 from pKK67-4 & pBR322
RC   pKK92c-2 from pKK84-1 & linker
RC   pKK161-8 from pKK92c-2 & M13mp8
RC   pKK175-6 from pKK161-8 & terminator T1
RC   pKK231-1 from pKK175-6 & pKK9-4 & pCM71
RC   pKK232-8 from pKK231-1 & pKK3535
RC   pKK122-3 from pKK92c-2 & pKK3535
RC   pKK100-17 from pKK92c-2 & pKK3535
RA   Brosius J.;
RT   "Plasmid vectors for the selection of promoters";
RL   Gene 27:151-160(1984).
XX
CC   NM (pKK9-4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(E.coli DH1)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pKK353-5)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 AvaI 4361bp 1426..1426
FT                   fill in
FT                   2. pKK3535 AluI-AluI 498bp 6720..7218,
FT                   \ E. coli rrnB term 497bp 6416..6913
FT                   -> pKK9-4 5285bp"
FT   misc_feature    0..0
FT                   /note="insert rrnB transcription terminator fragments
FT                   3' to TET gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             86..1276
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   CDS             1426..1921
FT                   /note="GEN E. coli T1, T2, & 5S genes"
FT   CDS             1922..2419
FT                   /note="GEN E. coli T1, T2, & 5S genes"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB transcription terminator"
FT   CDS             complement(4287..5147)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 5285 BP; 1220 A; 1446 C; 1395 G; 1224 T; 0 other;
     agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc
     ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc
     gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg
     caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc
     ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc
     ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat gcccttgaga
     gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt
     ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc
     ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc
     gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg
     ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc gctgatcgtc
     acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc
     gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
     acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
     atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac atatccatcg
     cgtccgccat ctccagcagc cgcacgcggc gcatcctgtt ttggcggatg agagaagatt
     ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
     ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
     agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
     aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
     cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc
     catcctgacg gatggccttt ttgcgtttct acaaactctt cctgtcgtca tatctacaag
     ccatcccccc acagatacgg taaactagcc tcgtttttgc atcaggaaca gctgttttgg
     cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttcctg
     tcgtcatatc tacaagccat ccccccacag atacggtaaa ctagcctcgt ttttgcatca
     ggaaagcagg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt
     gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg
     cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat
     ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat
     tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt
     attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac
     cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg
     tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaattcccc
     cttacacgga ggcatcaagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag
     gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg
     cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
     ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     aagaa
//