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ID   PKLY1      preliminary; circular DNA; SYN; 5107 BP.
XX
AC   IG9832;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKLY1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKLY1 from pKK233-2 & pEMBL8, f1 ori
RC   pKLY2 from pKLY1
RC   pKLY3 from pKLY2 & oligo, ColE2-P9 celB gene
RC   pKLXP2, pKLYP2 from pKLY3 & oligo
RC   pKLYPHOA, pKLXPHOA from pKLY3 & pCH2, PhoA gene
RA   Rioux C.R., Bergeron H., Lin L., Grothe S., O'Connor-McCourt M.,
RA   Lau P.C.;
RT   "A fusion plasmid for the synthesis of lipopeptide-antigen chimeras
RT   in Escherichia coli";
RL   Gene 116:13-20(1992).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pKLY1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pKK233-2)(pEMBL8+)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKK233-2 PvuII 4593bp 1697..1697
FT                   calf intestinal phosphatase
FT                   2. pEMBL8+ RsaI-RsaI 514bp 771..1285, f1 ori
FT                   -> pKLY1 5107bp"
FT   -               1..1696
FT                   /note="pKK233-2 1..1696 1696bp
FT                   PvuII = CAG^CTG
FT                   RsaI =   GT^AC"
FT   -               1697..2210
FT                   /note="pEMBL8+ 771..1284 514bp
FT                   RsaI =   GT^AC
FT                   PvuII = CAG^CTG"
FT   -               2211..5107
FT                   /note="pKK233-2 1697..4593 2897bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI phage f1 ori"
FT   misc_binding    0..0
FT                   /note="SIT SspI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   promoter        0..0
FT                   /note="GEN E. coli trc promoter"
FT   misc_binding    0..0
FT                   /note="SIT SspI"
XX
SQ   Sequence 5107 BP; 1157 A; 1399 C; 1302 G; 1249 T; 0 other;
     ccggatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgccgttgc
     tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc
     cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat
     gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt
     cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg
     aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga
     gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt
     cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg
     gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct
     tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac
     ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
     cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat
     caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg
     tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg
     gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact
     ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg
     tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa
     acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg
     ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt
     tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac
     cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc
     attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa
     aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
     tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
     atgagcttta ccgcagacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttaaatattt
     gcttatacaa tcttcctgtt tttggggctt ttctgattat caaccggggt ctgcctcgcg
     cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     ataaacaaaa agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     aatttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     cgcaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     ggcagttccc tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca
     cttctgagtt cggcatgggg tcaggtggga ccaccgcgct actgccgcca ggcaaactgt
     tttatcagac cgcttctgcg ttctgattta atctgtatca ggctgaaaat cttctctcat
     ccgccaaaac agccaagctt ggctgcagcc atggtctgtt tcctgtgtga aattgttatc
     cgctcacaat tccacacatt atacgagccg gatgattaat tgtcaacagc tcatttcaga
     atatttgcca gaaccgttta tatgtcggcg caaaaaacat tatccagaac gggagtgcgc
     cttgagcgac acgaattatg cagtgattta cgacctgcac agccaatcca cagcttccga
     tggctgcctg acgccagaag cattggtgca ccgtgcagtc gatgataagc tgtcaaacat
     gagaatt
//