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ID PKM1 preliminary; circular DNA; SYN; 4378 BP.
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AC L08921; VB0124; ATCC37285;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pKM1 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pKM-1 from pKO-1
RC pPCV from pBR322 & linker
RC pBR322tacI from pHGH207-1 & pHGH107-11 & pBR322
RC pHGH807tacI from pBR322tacI & pHGH207-1
RC pHGH907tacII from pBR322tacI & pHGH207-1
RC pKM-lac from pKM-1 & pHGH107-11
RC pKM-trp from pKM-1 & pHGH207-1
RC pKM-tacI from pKM-1 & pHGH807tacI
RC pKM-tacII from pKM-1 & pHGH907tacII
RA De Boer H.A., Comstock L.J., Vasser M.;
RT "The tac promoter: a functional hybrid derived from the trp and lac
RT promoters";
RL Proc. Natl. Acad. Sci. U.S.A. 80:21-25(1983).
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RN [2]
RP 1-4378
RC pKM-1
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
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CC Assembled from pKO1 and phage lambda by F. Pfeiffer.
CC This is a promoter probe vector. The promoterless galK gene is
CC used as selectable marker. Translationsal stop codons in all reading
CC frames are present on a 11-bp oligonucleotide before the galKgene.
CC Also, a polylinker was cloned before this gene. In addition, the
CC lambda tr1 transcription terminator was inserted BEFORE the galK gene
CC to allow measurement of very strong promoters due to read-through
CC transcription.
CC
CC Promoter-cloning vector for isolating and characterizing very strong
CC promoters. [1]
CC This vector was constructed by inserting a rho-dependent terminator
CC (lambdaTRI) into the SmaI site of pKO1 (ATCC 37126). This reduces the
CC number of transcripts reaching the galK coding sequences to 30% or
CC less & is thus useful for strong promoters. [2]
CC Medium is 1227 LB plus ampicillin.
CC NM (pKM1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (E.coli N100)(E.coli)
CC CP ()
CC FN (promoter analysis)
CC SE ()
CC PA (EcoGalK)(pBR322)(pKO-1)(lambda)
CC BR ()
CC OF (pKM2)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pKO-1 SmaI 3980bp 312..312
FT 2. lambda SmaI-SmaI 400bp, tR1 rho-dependent term
FT -> pKM-1 4378bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_feature 1..294
FT /note="from lambda"
FT misc_feature 312..709
FT /note="from lambda"
FT CDS 721..2083
FT /note="GEN E. coli galactokinase gene (galK)"
FT misc_feature 2084..4378
FT /note="from pBR322"
FT CDS 3313..4101
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 4378 BP; 1122 A; 1111 C; 1120 G; 1025 T; 0 other;
ttctggcgaa tcctctgacc agccagaaaa cgacctttct gtggtgaaac cggatgctgc
aattcagagc gccagcaagt gggggacagc agaagacctg accgccgcag agtggatgtt
tgacatggtg aagactatcg caccatcagc cagaaaaccg aattttgctg ggtgggctaa
cgatatccgc ctgatgcgtg aacgtgacgg acgtaaccac cgcgacatgt gtgtgctgtt
ccgctgggca tgccaggaca acttctggtc cggtaacgtg ctgagcccgg ccaagcttac
tccccatccc cccgaaagat ttttttaact ataaacgctg atggaagcgt ttatgcggaa
gaggtaaagc ccttcccgag taacaaaaaa acaacagcat aaataacccc gctcttacac
attccagccc tgaaaaaggg catcaaatta aaccacacct atggtgtatg catttatttg
catacattca atcaattgtt atctaaggaa atacttacat atggttcgtg caaacaaacg
caacgaggct ctacgaatcg agagtgcgtt gcttaacaaa atcgcaatgc ttggaactga
gaagacagcg gaagctgtgg gcgttgataa gtcgcagatc agcaggtgga agagggactg
gattccaaag ttctcaatgc tgcttgctgt tcttgaatgg ggggtcgttg ggcaataagg
gctgcacgcg cacttttatc cgcctctgct gcgctccgcc accgtacgta aatttatggt
tggttatgaa atgctggcag agacccagcg agacctgacc gcagaacagg cagcagagcg
tttgcgcgca gtcagcgata tccattttcg cgaatccgga gtgtaagaaa tgagtctgaa
agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc acaccattca
ggcgcctggc cgcgtgaatt tgattggtga acacaccgac tacaacgacg gtttcgttct
gccctgcgcg attgattatc aaaccgtgat cagttgtgca ccacgcgatg accgtaaagt
tcgcgtgatg gcagccgatt atgaaaatca gctcgacgag ttttccctcg atgcgcccat
tgtcgcacat gaaaactatc aatgggctaa ctacgttcgt ggcgtggtga aacatctgca
actgcgtaac aacagcttcg gcggcgtgga catggtgatc agcggcaatg tgccgacggg
tgccgggtta agttcttccg cttcactgga agtcgcggtc ggaaccgtat tgcagcagct
ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac ggtcaggaag cagaaaacca
gtttgtaggc tgtaactgcg ggatcatgga tcagctaatt tccgcgctcg gcaagaaaga
tcatgccttg ctgatcgatt gccgctcact ggggaccaaa gcagtttcca tgcccaaagg
tgtggctgtc gtcatcatca acagtaactt caaacgtacc ctggttggca gcgaatacaa
cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag ccctgcgtga
tgtcaccatt gaagagttca acgctgttgc gcatgaactg gacccgatcg tggcaaaacg
cgtgcgtcat atactgactg aaaacgcccg caccgttgaa gctgccagcg cgctggagca
aggcgacctg aaacgtatgg gcgagttgat ggcggagtct catgcctcta tgcgcgatga
tttcgaaatc accgtgccgc aaattgacac tctggtagaa atcgtcaaag ctgtgattgg
cgacaaaggt ggcgtacgca tgaccggcgg cggatttggc ggctgtatcg tcgcgctgat
cccggaagag ctggtgcctg ccgcacagca agctgtcgct gaacaatatg aagcaaaaac
aggtattaaa gagacttttt acgtttgtaa accatcacaa ggagcaggac agtgctgaac
gaaactcccg cactggcacc cgatggcagc cgtaccgact gttctgcctc gcgcgtttcg
gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa
//