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ID   PKM1KAN    preliminary; circular DNA; SYN; 5876 BP.
XX
AC   IG9971;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKm1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pBR322ubi from pBR322 & lambda HUb13, human UbC promoter
RC   plasmid from pIC19R & pBR322ubi & linker
RC   plasmid2 from pML & plasmid
RC   plasmid3 from pIC19R & pKm2, kan gene
RC   plasmid4 from plasmid2 & plasmid3
RC   plasmid5 from plasmid4 & linker & pNPY3-75, neuropeptide Y precursor
RC   p753 from plasmid5 & SV40 polyA
RA   Wulff B.S., O'Hare M.M.T., Boel E., Theill L.E., Schwartz T.W.;
RT   "Partial processing of the neuropeptide Y precursor in transfected
RT   CHO cells";
RL   FEBS Lett. 261:101-105(1990).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pKm1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(Tn5)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI-HindIII 376..4361..30 4015bp
FT                   2. Tn5 HindIII-BamHI 1861bp 1196..3057,
FT                   \ neo/kan gene with BglII
FT                   -> pKm1 5876bp"
FT   -               1..29
FT                   /note="pBR322 1..29 29bp
FT                   HindIII = A^AGCTT"
FT   -               30..1890
FT                   /note="Tn5 1196..3056 1861bp
FT                   BamHI = G^GATCC"
FT   -               1891..5876
FT                   /note="pBR322 376..4361 3986bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 5876 BP; 1272 A; 1638 C; 1649 G; 1317 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gcttcacgct gccgcaagca ctcagggcgc
     aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
     cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
     caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
     agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa
     gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     gggatctcat gctggagttc ttcgcccacc ccgggctcga tcccctcgcg agttggttca
     gctgctgcct gaggctggac gacctcgcgg agttctaccg gcagtgcaaa tccgtcggca
     tccaggaaac cagcagcggc tatccgcgca tccatgcccc cgaactgcag gagtggggag
     gcacgatggc cgctttggtc gacccggacg ggacgctcct gcgcctgata cagaacgaat
     tgcttgcagg catctcatga gtgtgtcttc ccgttttccg cctgaggtca ctgcgtggat
     ggagcgctgg cgcctgctgc gcgacggcga gctgctcacc acccactcga gctggatact
     tcccgtccgc cagggggaca tgccggcgat gctgaaggtc gcgcgcattc ccgatgaaga
     ggccggttac cgcctgttga cctggtggga cgggcagggc gccgcccgag tcttcgcctc
     ggcggcgggc gctctgctca tggagcgcgc gtccggggcc ggggaccttg cacagatagc
     gtggtccggc caggacgacg aggcttgcag gatcctctac gccggacgca tcgtggccgg
     catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc
     ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat
     gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac
     tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga
     cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat
     gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
     ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac
     ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa
     cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg
     cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga
     ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc
     aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc
     tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
     cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
     aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
     tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg
     agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg
     gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
     tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
     gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaa
//