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ID   PKO1       preliminary; circular DNA; SYN; 3980 BP.
XX
AC   L08923; VB0122; IG0073; ATCC37126;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKO-1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [pBR322-Tc from pBR322 & trp promoter]
RC   pKO-1 from pBR322-Tc & galK gene
RC   pKG1800 from pKO-1 & gal operon promoter
RC   pKG1900 from pKO-1 & gal operon promoter
RC   [lambda gal8 from lambda & gal operon promoter]
RC   pKB-2000 from pKO-1 & lambda gal8
RC   pKO-4, pKO-6, pKO-11 from pKO-1
RA   McKenney K., Shimatake H., Court D., Schmeissner U., Brady C.,
RA   Rosenberg M.;
RT   "A system to study promoter and terminator signals recognized by
RT   Escherichia coli RNA polymerase";
RL   Gene Amplif. Anal. 2:383-415(1981).
RL   Chirikjian J.G., Papas T.J.;
RL   (eds.), Elsevier, North Holland.
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RN   [2]
RC   from pBRH series
RC   from pKG series
RC   from pKO series
RC   from pKT series
RC   from pMMB series
RC   from pPV series
RA   Nierman W.C.;
RT   "Vectors for cloning promoters and terminators";
RL   Vectors 0:153-177(1988).
RL   Rodriguez R.L., Denhardt D.T., eds., Butterworths, Boston.
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RN   [3]
RP   1-3980
RC   pKO-1
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [4]
RC   px1, px2 from pBR322 & HSV 1 thymidine kinase gene
RA   Enquist L.W., Vande Woude G.F., Wagner M., Smiley J.R., Summers W.C.;
RT   "Construction and characterization of recombinant plasmid encoding
RT   the gene for the thymidiene kinase of Herpes simplex type 1 virus";
RL   Gene 7:335-342(1979).
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RN   [5]
RC   pA11 from pBR322
RC   pA11P from linker & pA11
RC   pA11-SV2353 from pA11 & SV40
RC   pA11-SVL2 from pA11-SV2353
RA   Gruss P., Rosenthal W., Konig M., Ellis R.W., Shih T.Y.,
RA   Scolnick E.M., Khoury G.;
RT   "The expression of viral extracellular p21 ras genes using SV40 as
RT   a vector";
RL   Eukaryotic Viral Vectors 0:13-17(1982).
RL   Guzman Y., ed.;
RL   Cold Spring Harbor Laboratory, Cold Spring Harbor NY.
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RN   [6]
RC   pKO-1
RA   Enger-Valk B.E.;
RT   ;
RL   Submitted (00-XXX-1985) on floppy disk by:
RL   Enger-Valk B.E., .
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Revised according to sequence of pBR322 by F. Pfeiffer
CC   This is a promoter probe vector. The promoterless galK gene is used as
CC   a selectable marker. Translational stop codons are present before
CC   the galK gene in all three reading frames.
CC
CC   A promoter cloning plasmid vector. (ATCC staff)
CC   The vector contains a promoterless galactokinase (galK) gene.  There
CC   are translation stop codons in all three reading frames between the
CC   cloning sites and the galK sequences. [4]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKO-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli GALK-)(bacteria GalE-T-K-)(E.coli N100)(E.coli)
CC   CP ()
CC   FN (promoter analysis)
CC   SE (color red/white)
CC   PA (pBR322)(E.coli galK)(lambda)
CC   BR ()
CC   OF (pKO-2)(pKM1)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-PvuII 2068bp
FT                   \ 4360..4361..2067, tet gene/2293bp
FT                   2. E.coli EcoRI-PvuII, promoterless galK gene
FT                   -> pKO-1 3980bp"
FT   CDS             0..0
FT                   /note="GEN lambda O gene"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   CDS             462..1608
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2915..3703)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI"
FT   misc_feature    1..294
FT                   /note="from bacteriophage lambda"
FT   CDS             323..1685
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   misc_feature    1686..3980
FT                   /note="from pBR322"
XX
SQ   Sequence 3980 BP; 994 A; 1031 C; 1029 G; 926 T; 0 other;
     ttctggcgaa tcctctgacc agccagaaaa cgacctttct gtggtgaaac cggatgctgc
     aattcagagc gccagcaagt gggggacagc agaagacctg accgccgcag agtggatgtt
     tgacatggtg aagactatcg caccatcagc cagaaaaccg aattttgctg ggtgggctaa
     cgatatccgc ctgatgcgtg aacgtgacgg acgtaaccac cgcgacatgt gtgtgctgtt
     ccgctgggca tgccaggaca acttctggtc cggtaacgtg ctgagcccgg ccaagcttac
     tccccatccc cgggcaataa gggctgcacg cgcactttta tccgcctctg ctgcgctccg
     ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga
     ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg
     gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct
     accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg
     actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagttgtg
     caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg
     agttttccct cgatgcgccc attgtcgcac atgaaaacta tcaatgggct aactacgttc
     gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggcgtg gacatggtga
     tcagcggcaa tgtgccgacg ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg
     tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta
     acggtcagga agcagaaaac cagtttgtag gctgtaactg cgggatcatg gatcagctaa
     tttccgcgct cggcaagaaa gatcatgcct tgctgatcga ttgccgctca ctggggacca
     aagcagtttc catgcccaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta
     ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct
     tccagcagcc agccctgcgt gatgtcacca ttgaagagtt caacgctgtt gcgcatgaac
     tggacccgat cgtggcaaaa cgcgtgcgtc atatactgac tgaaaacgcc cgcaccgttg
     aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggcggagt
     ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag
     aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg
     gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgcacag caagctgtcg
     ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac
     aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggca gccgtaccga
     ctgttctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
     tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     ggccctttcg tcttcaagaa
//