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ID   PKO10      preliminary; circular DNA; SYN; 3853 BP.
XX
AC   IG0074;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKO-10 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pBR322-Tc from pBR322 & trp promoter]
RC   pKO-1 from pBR322-Tc & galK gene
RC   pKG1800 from pKO-1 & gal operon promoter
RC   pKG1900 from pKO-1 & gal operon promoter
RC   [lambda gal8 from lambda & gal operon promoter]
RC   pKB-2000 from pKO-1 & lambda gal8
RC   pKO-4, pKO-6, pKO-11 from pKO-1
RA   McKenney K., Shimatake H., Court D., Schmeissner U., Brady C.,
RA   Rosenberg M.;
RT   "A system to study promoter and terminator signals recognized by
RT   Escherichia coli RNA polymerase";
RL   Gene Amplif. Anal. 2:383-415(1981).
RL   Chirikjian J.G., Papas T.J.;
RL   (eds.), Elsevier, North Holland.
XX
CC   NM (pKO-10)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli GALK-)(bacteria GalE-T-K-)
CC   CP ()
CC   FN (promoter analysis)
CC   SE (color red/white)
CC   PA (pKO-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKO-1 EcoRI 3980bp 3979..3979
FT                   SalI linker 6bp gtcgac
FT                   SmaI 3980bp 312..312
FT                   SmaI-BamHI-EcoRI linker 18bp cccgggggatccgaattc
FT                   -> pKO-10 3853bp"
FT   CDS             0..0
FT                   /note="GEN lambda O gene"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   CDS             478..1624
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   rep_origin      2090..2090
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2855..3715)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3853 BP; 964 A; 991 C; 994 G; 904 T; 0 other;
     cagagcgcca gcaagtgggg gacagcagaa gacctgaccg ccgcagagtg gatgtttgac
     atggtgaaga ctatcgcacc atcagccaga aaaccgattt tgctgggtgg gctaacgata
     tccgcctgat gcgtgaacgt gacggacgta accaccgcga catgtgtgtg ctgttccgct
     gggcatgcca ggacaacttc tggtccggta acgtgctgag cccggccaag cttactcccc
     atccccggga tccgaattcc aataagggct gcacgcgcac ttttatccgc ctctgctgcg
     ctccgccacc gtacgtaaat ttatggttgg ttatgaaatg ctggcagaga cccagcgaga
     cctgacagtc agcgatatcc attttcgcga atccggagtg taagaaatga gtctgaaaga
     aaaaacacaa tctctgtttg ccaacgcatt tggctaccct gccactcaca ccattcaggc
     gcctggccgc gtgaatttga ttggtgaaca caccgactac aacgacggtt tcgttctgcc
     ctgcgcgatt gattatcaaa ccgtgatcag ttgtgcacca cgcgatgacc gtaaagttcg
     cgtgatggca gccgattatg aaaatcagct cgacgagttt tccctcgatg cgcccattgt
     cgcacatgaa aactatcaat gggctaacta cgttcgtggc gtggtgaaac atctgcaact
     gcgtaacaac agcttcggcg gcgtggacat ggtgatcagc ggcaatgtgc cgcagggtgc
     cgggttaagt tcttccgctt cactggaagt cgcggtcgga accgtattgc agcagcttta
     tcatctgccg ctggacggcg cacaaatcgc gcttaacggt caggaagcag aaaaccagtt
     tgtaggctgt aactgcggga tcatggatca gctaatttcc gcgctcggca agaaagatca
     tgccttgctg atcgattgcc gctcactggg gaccaaagca gtttccatgc ccaaaggtgt
     ggctgtcgtc atcatcaaca gtaacttcaa acgtaccctg gttggcagcg aatacaacac
     ccgtcgtgaa cagtgcgaaa ccggtgcgcg tttcttccag cagccagccc tgcgtgatgt
     caccattgaa gagttcaacg ctgttgcgca tgaactggac ccgatcgtgg caaaacgcgt
     gcgtcatata ctgactgaaa acgcccgcac cgttgaagct gccagcgcgc tggagcaagg
     cgacctgaaa cgtatgggcg agttgatggc ggagtctcat gcctctatgc gcgatgattt
     cgaaatcacc gtgccgcaaa ttgacactct ggtagaaatc gtcaaagctg tgattggcga
     caaaggtggc gtacgcatga ccggcggcgg atttggcggc tgtatcgtcg cgctgatccc
     ggaagagctg gtgcctgccg tacagcaagc tgtcgctgaa caatatgaag caaaaacagg
     tattaaagag actttttacg tttgtaaacc atcacaagga gcaggacagt gctgacagct
     gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     tcgtcttcaa gaa
//