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ID   PKO100     preliminary; circular DNA; SYN; 3995 BP.
XX
AC   ATCC37123;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKO100 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [pBR322-Tc from pBR322 & trp promoter]
RC   pKO-1 from pBR322-Tc & galK gene
RC   pKG1800 from pKO-1 & gal operon promoter
RC   pKG1900 from pKO-1 & gal operon promoter
RC   [lambda gal8 from lambda & gal operon promoter]
RC   pKB-2000 from pKO-1 & lambda gal8
RC   pKO-4, pKO-6, pKO-11 from pKO-1
RA   McKenney K., Shimatake H., Court D., Schmeissner U., Brady C.,
RA   Rosenberg M.;
RT   "A system to study promoter and terminator signals recognized by
RT   Escherichia coli RNA polymerase";
RL   Gene Amplif. Anal. 2:383-415(1981).
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RN   [2]
RC   pKO-9 from M13mp7 & pKO-4
RC   pKO-11 from pUC9 & pKO-1
RC   pCAP1P2 from pKO-9 & ilv gene promoter
RC   pCAP2 from pKO-9 & P2 gene promoter
RC   pCAP1 from pKO-11 & P1 gene promoter
RC   pCA1100 from pKO-1
RA   Adams C.W., Hatfield G.W.;
RT   "Effects of promoter strengths and growth conditions on copy
RT   number of transcription-fusion vectors";
RL   J. Biol. Chem. 259:7399-7403(1984).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   This is a promoter cloning vector using expression of the promoterless
CC   galK gene for positive or color (MacConkey galactose indicator plates)
CC   selection. [3]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKO100)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JA221)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pKO-1)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKO-1 SmaI 3980bp 312..312
FT                   linker SmaI-BamHI-EcoRI-SmaI 15bp gggatccgaattccc
FT                   -> pKO-100 3995bp"
FT   -               1..311
FT                   /note="pKO-1 1..311 311bp
FT                   SmaI = XmaI = CCC^GGG
FT                   \                 gggatccgaattccc"
FT   -               312..326
FT                   /note="gggatccgaattccc 15bp
FT                   \ gggatccgaattccc
FT                   SmaI = XmaI = CCC^GGG"
FT   -               327..3995
FT                   /note="pKO-1 312..3980 3669bp"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-SmaI-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN E. coli galactokinase gene (galK);
FT                   reporter gene"
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SQ   Sequence 3995 BP; 997 A; 1036 C; 1033 G; 929 T; 0 other;
     ttctggcgaa tcctctgacc agccagaaaa cgacctttct gtggtgaaac cggatgctgc
     aattcagagc gccagcaagt gggggacagc agaagacctg accgccgcag agtggatgtt
     tgacatggtg aagactatcg caccatcagc cagaaaaccg aattttgctg ggtgggctaa
     cgatatccgc ctgatgcgtg aacgtgacgg acgtaaccac cgcgacatgt gtgtgctgtt
     ccgctgggca tgccaggaca acttctggtc cggtaacgtg ctgagcccgg ccaagcttac
     tccccatccc cgggatccga attcccgggc aataagggct gcacgcgcac ttttatccgc
     ctctgctgcg ctccgccacc gtacgtaaat ttatggttgg ttatgaaatg ctggcagaga
     cccagcgaga cctgaccgca gaacaggcag cagagcgttt gcgcgcagtc agcgatatcc
     attttcgcga atccggagtg taagaaatga gtctgaaaga aaaaacacaa tctctgtttg
     ccaacgcatt tggctaccct gccactcaca ccattcaggc gcctggccgc gtgaatttga
     ttggtgaaca caccgactac aacgacggtt tcgttctgcc ctgcgcgatt gattatcaaa
     ccgtgatcag ttgtgcacca cgcgatgacc gtaaagttcg cgtgatggca gccgattatg
     aaaatcagct cgacgagttt tccctcgatg cgcccattgt cgcacatgaa aactatcaat
     gggctaacta cgttcgtggc gtggtgaaac atctgcaact gcgtaacaac agcttcggcg
     gcgtggacat ggtgatcagc ggcaatgtgc cgacgggtgc cgggttaagt tcttccgctt
     cactggaagt cgcggtcgga accgtattgc agcagcttta tcatctgccg ctggacggcg
     cacaaatcgc gcttaacggt caggaagcag aaaaccagtt tgtaggctgt aactgcggga
     tcatggatca gctaatttcc gcgctcggca agaaagatca tgccttgctg atcgattgcc
     gctcactggg gaccaaagca gtttccatgc ccaaaggtgt ggctgtcgtc atcatcaaca
     gtaacttcaa acgtaccctg gttggcagcg aatacaacac ccgtcgtgaa cagtgcgaaa
     ccggtgcgcg tttcttccag cagccagccc tgcgtgatgt caccattgaa gagttcaacg
     ctgttgcgca tgaactggac ccgatcgtgg caaaacgcgt gcgtcatata ctgactgaaa
     acgcccgcac cgttgaagct gccagcgcgc tggagcaagg cgacctgaaa cgtatgggcg
     agttgatggc ggagtctcat gcctctatgc gcgatgattt cgaaatcacc gtgccgcaaa
     ttgacactct ggtagaaatc gtcaaagctg tgattggcga caaaggtggc gtacgcatga
     ccggcggcgg atttggcggc tgtatcgtcg cgctgatccc ggaagagctg gtgcctgccg
     cacagcaagc tgtcgctgaa caatatgaag caaaaacagg tattaaagag actttttacg
     tttgtaaacc atcacaagga gcaggacagt gctgaacgaa actcccgcac tggcacccga
     tggcagccgt accgactgtt ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     ataggcgtat cacgaggccc tttcgtcttc aagaa
//