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ID   PKO11      preliminary; circular DNA; SYN; 3935 BP.
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AC   IG0075; ATCC37122;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKO-11 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pKO-9 from M13mp7 & pKO-4
RC   pKO-11 from pUC9 & pKO-1
RC   pCAP1P2 from pKO-9 & ilv gene promoter
RC   pCAP2 from pKO-9 & P2 gene promoter
RC   pCAP1 from pKO-11 & P1 gene promoter
RC   pCA1100 from pKO-1
RA   Adams C.W., Hatfield G.W.;
RT   "Effects of promoter strengths and growth conditions on copy number
RT   of transcription-fusion vectors";
RL   J. Biol. Chem. 259:7399-7403(1984).
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CC   This is a promoter cloning vector using expression of the promoterless
CC   galK gene for positive or color (MacConkey galactose indicator plates)
CC   selection. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKO-11)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli GALK-)(bacteria GalE-T-K-)(E.coli JA221)(E.coli N100)
CC   HO (E.coli N99)(E.coli)
CC   CP ()
CC   FN (promoter analysis)
CC   SE (color red/white)
CC   PA (pKO-1)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKO-4 3842bp
FT                   2. M13mp7 PstI-SalI-BamHI-SmaI-EcoRI 17bp 6257..6274,
FT                   \ MCS
FT                   -> pKO-11 3935bp"
FT   CDS             0..0
FT                   /note="GEN lambda O gene"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   CDS             489..1635
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   rep_origin      2172..2172
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2937..3797)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="MCS NruI-AvaI-SmaI-XmaI-BamHI-SalI-HindIII"
XX
SQ   Sequence 3935 BP; 977 A; 1013 C; 1018 G; 918 T; 9 other;
     gccagcaagt gggggacagc agaagacctg accgccgcag agtggatgtt tgacatggtg
     aagactatcg caccatcagc cagaaaaccg attttgctgg gtgggctaac gatatccgcc
     tgatgcgtga acgtgacgga cgtaaccacc gcgacatgtg tgtgctgttc cgctgggcat
     gccaggacaa cttctggtcc ggtaacgtgc tgagcccggc caagcttact ccccatcccc
     ctgcagtcga cggatcccgg ggaattcggg caataagggc tgcacgcgca cttttatccg
     cctctgctgc gctccgccac cgtacgtaaa tttatggttg gttatgaaat gctggcagag
     acccagcgag acctgacagt cagcgatatc cattttcgcg aatccggagt gtaagaaatg
     agtctgaaag aaaaaacaca atctctgttt gccaacgcat ttggctaccc tgccactcac
     accattcagg cgcctggccg cgtgaatttg attggtgaac acaccgacta caacgacggt
     ttcgttctgc cctgcgcgat tgattatcaa accgtgatca gttgtgcacc acgcgatgac
     cgtaaagttc gcgtgatggc agccgattat gaaaatcagc tcgacgagtt ttccctcgat
     gcgcccattg tcgcacatga aaactatcaa tgggctaact acgttcgtgg cgtggtgaaa
     catctgcaac tgcgtaacaa cagcttcggc ggcgtggaca tggtgatcag cggcaatgtg
     ccgcagggtg ccgggttaag ttcttccgct tcactggaag tcgcggtcgg aaccgtattg
     cagcagcttt atcatctgcc gctggacggc gcacaaatcg cgcttaacgg tcaggaagca
     gaaaaccagt ttgtaggctg taactgcggg atcatggatc agctaatttc cgcgctcggc
     aagaaagatc atgccttgct gatcgattgc cgctcactgg ggaccaaagc agtttccatg
     cccaaaggtg tggctgtcgt catcatcaac agtaacttca aacgtaccct ggttggcagc
     gaatacaaca cccgtcgtga acagtgcgaa accggtgcgc gtttcttcca gcagccagcc
     ctgcgtgatg tcaccattga agagttcaac gctgttgcgc atgaactgga cccgatcgtg
     gcaaaacgcg tgcgtcatat actgactgaa aacgcccgca ccgttgaagc tgccagcgcg
     ctggagcaag gcgacctgaa acgtatgggc gagttgatgg cggagtctca tgcctctatg
     cgcgatgatt tcgaaatcac cgtgccgcaa attgacactc tggtagaaat cgtcaaagct
     gtgattggcg acaaaggtgg cgtacgcatg accggcggcg gatttggcgg ctgtatcgtc
     gcgctgatcc cggaagagct ggtgcctgcc gtacagcaag ctgtcgctga acaatatgaa
     gcaaaaacag gtattaaaga gactttttac gtttgtaaac catcacaagg agcaggacag
     tgctgacagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     cccggagann nnnnnnncag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     ataggcgtat cacgaggccc tttcgtcttc aagaa
//