ID   PKO4       preliminary; circular DNA; SYN; 3842 BP.
XX
AC   IG0077;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKO-4 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pBR322-Tc from pBR322 & trp promoter]
RC   pKO-1 from pBR322-Tc & galK gene
RC   pKG1800 from pKO-1 & gal operon promoter
RC   pKG1900 from pKO-1 & gal operon promoter
RC   [lambda gal8 from lambda & gal operon promoter]
RC   pKB-2000 from pKO-1 & lambda gal8
RC   pKO-3, pKO-4, pKO-6, pKO-11 from pKO-1
RA   McKenney K., Shimatake H., Court D., Schmeissner U., Brady C.,
RA   Rosenberg M.;
RT   "A system to study promoter and terminator signals recognized by
RT   Escherichia coli RNA polymerase";
RL   Gene Amplif. Anal. 2:383-415(1981).
RL   Chirikjian J.G., Papas T.J.;
RL   (eds.), Elsevier, North Holland.
XX
CC   NM (pKO-4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli GALK-)(bacteria GalE-T-K-)
CC   CP ()
CC   FN (promoter analysis)
CC   SE (color red/white)
CC   PA (pKO-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKO-1 SmaI 3980bp 312..312
FT                   BamHI linker 6bp ggatcc
FT                   -> pKO-4 3842bp"
FT   CDS             0..0
FT                   /note="GEN lambda O gene"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   CDS             468..1614
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   rep_origin      2308..2308
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2845..3705)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3842 BP; 947 A; 994 C; 1021 G; 880 T; 0 other;
     gccagcaagt gggggacagc agaagacctg accgccgcag agtggatgtt tgacatggtg
     aagactatcg caccatcagc cagaaaaccg attttgctgg gtgggctaac gatatccgcc
     tgatgcgtga acgtgacgga cgtaaccacc gcgacatgtg tgtgctgttc cgctgggcat
     gccaggacaa cttctggtcc ggtaacgtgc tgagcccggc caagcttact ccccatcccc
     ggatccgggc aataagggct gcacgcgcac ttttatccgc ctctgctgcg ctccgccacc
     gtacgtaaat ttatggttgg ttatgaaatg ctggcagaga cccagcgaga cctgacagtc
     agcgatatcc attttcgcga atccggagtg taagaaatga gtctgaaaga aaaaacacaa
     tctctgtttg ccaacgcatt tggctaccct gccactcaca ccattcaggc gcctggccgc
     gtgaatttga ttggtgaaca caccgactac aacgacggtt tcgttctgcc ctgcgcgatt
     gattatcaaa ccgtgatcag ttgtgcacca cgcgatgacc gtaaagttcg cgtgatggca
     gccgattatg aaaatcagct cgacgagttt tccctcgatg cgcccattgt cgcacatgaa
     aactatcaat gggctaacta cgttcgtggc gtggtgaaac atctgcaact gcgtaacaac
     agcttcggcg gcgtggacat ggtgatcagc ggcaatgtgc cgcagggtgc cgggttaagt
     tcttccgctt cactggaagt cgcggtcgga accgtattgc agcagcttta tcatctgccg
     ctggacggcg cacaaatcgc gcttaacggt caggaagcag aaaaccagtt tgtaggctgt
     aactgcggga tcatggatca gctaatttcc gcgctcggca agaaagatca tgccttgctg
     atcgattgcc gctcactggg gaccaaagca gtttccatgc ccaaaggtgt ggctgtcgtc
     atcatcaaca gtaacttcaa acgtaccctg gttggcagcg aatacaacac ccgtcgtgaa
     cagtgcgaaa ccggtgcgcg tttcttccag cagccagccc tgcgtgatgt caccattgaa
     gagttcaacg ctgttgcgca tgaactggac ccgatcgtgg caaaacgcgt gcgtcatata
     ctgactgaaa acgcccgcac cgttgaagct gccagcgcgc tggagcaagg cgacctgaaa
     cgtatgggcg agttgatggc ggagtctcat gcctctatgc gcgatgattt cgaaatcacc
     gtgccgcaaa ttgacactct ggtagaaatc gtcaaagctg tgattggcga caaaggtggc
     gtacgcatga ccggcggcgg atttggcggc tgtatcgtcg cgctgatccc ggaagagctg
     gtgcctgccg tacagcaagc tgtcgctgaa caatatgaag caaaaacagg tattaaagag
     actttttacg tttgtaaacc atcacaagga gcaggacagt gctgacagct gccgcaaatt
     gacactctgg tagaaatcgt caaagctgtg attggcgaca aaggtggcgt acgcatgacc
     ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg aagagctggt gcctgccgta
     cagcaagctg tcgctgaaca atatgaagca aaaacaggta ttaaagagac tttttacgtt
     tgtaaaccat cacaaggagc aggacagtgc tgacagctgc ctcgcgcgtt tcggtgatga
     cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
     agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
     gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     ac
//