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ID   PKO7       preliminary; circular DNA; SYN; 3849 BP.
XX
AC   IG0080;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKO-7 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pBR322-Tc from pBR322 & trp promoter]
RC   pKO-1 from pBR322-Tc & galK gene
RC   pKG1800 from pKO-1 & gal operon promoter
RC   pKG1900 from pKO-1 & gal operon promoter
RC   [lambda gal8 from lambda & gal operon promoter]
RC   pKB-2000 from pKO-1 & lambda gal8
RC   pKO-3, pKO-4, pKO-6, pKO-11 from pKO-1
RA   McKenney K., Shimatake H., Court D., Schmeissner U., Brady C.,
RA   Rosenberg M.;
RT   "A system to study promoter and terminator signals recognized by
RT   Escherichia coli RNA polymerase";
RL   Gene Amplif. Anal. 2:383-415(1981).
RL   Chirikjian J.G., Papas T.J.;
RL   (eds.), Elsevier, North Holland.
XX
CC   NM (pKO-7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli GALK-)(bacteria GalE-T-K-)
CC   CP ()
CC   FN (promoter analysis)
CC   SE (color red/white)
CC   PA (pKO-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKO-1 EcoRI 3980bp 3979..3979
FT                   EcoRI-BamHI-EcoRI linker 18bp gaattcggatccgaattc
FT                   -> pKO-7 3849bp"
FT   CDS             0..0
FT                   /note="GEN lambda O gene"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   CDS             474..1620
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   rep_origin      2068..2068
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2851..3711)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3849 BP; 963 A; 989 C; 994 G; 903 T; 0 other;
     gcaattcaga gcgccagcaa gtgggggaca gcagaagacc tgaccgccgc agagtggatg
     tttgacatgg tgaagactat cgcaccatca gccagaaaac cgattttgct gggtgggcta
     acgatatccg cctgatgcgt gaacgtgacg gacgtaacca ccgcgacatg tgtgtgctgt
     tccgctgggc atgccaggac aacttctggt ccggtaacgt gctgagcccg gccaagctta
     ctccccatcc ccgggcaata agggctgcac gcgcactttt atccgcctct gctgcgctcc
     gccaccgtac gtaaatttat ggttggttat gaaatgctgg cagagaccca gcgagacctg
     acagtcagcg atatccattt tcgcgaatcc ggagtgtaag aaatgagtct gaaagaaaaa
     acacaatctc tgtttgccaa cgcatttggc taccctgcca ctcacaccat tcaggcgcct
     ggccgcgtga atttgattgg tgaacacacc gactacaacg acggtttcgt tctgccctgc
     gcgattgatt atcaaaccgt gatcagttgt gcaccacgcg atgaccgtaa agttcgcgtg
     atggcagccg attatgaaaa tcagctcgac gagttttccc tcgatgcgcc cattgtcgca
     catgaaaact atcaatgggc taactacgtt cgtggcgtgg tgaaacatct gcaactgcgt
     aacaacagct tcggcggcgt ggacatggtg atcagcggca atgtgccgca gggtgccggg
     ttaagttctt ccgcttcact ggaagtcgcg gtcggaaccg tattgcagca gctttatcat
     ctgccgctgg acggcgcaca aatcgcgctt aacggtcagg aagcagaaaa ccagtttgta
     ggctgtaact gcgggatcat ggatcagcta atttccgcgc tcggcaagaa agatcatgcc
     ttgctgatcg attgccgctc actggggacc aaagcagttt ccatgcccaa aggtgtggct
     gtcgtcatca tcaacagtaa cttcaaacgt accctggttg gcagcgaata caacacccgt
     cgtgaacagt gcgaaaccgg tgcgcgtttc ttccagcagc cagccctgcg tgatgtcacc
     attgaagagt tcaacgctgt tgcgcatgaa ctggacccga tcgtggcaaa acgcgtgcgt
     catatactga ctgaaaacgc ccgcaccgtt gaagctgcca gcgcgctgga gcaaggcgac
     ctgaaacgta tgggcgagtt gatggcggag tctcatgcct ctatgcgcga tgatttcgaa
     atcaccgtgc cgcaaattga cactctggta gaaatcgtca aagctgtgat tggcgacaaa
     ggtggcgtac gcatgaccgg cggcggattt ggcggctgta tcgtcgcgct gatcccggaa
     gagctggtgc ctgccgtaca gcaagctgtc gctgaacaat atgaagcaaa aacaggtatt
     aaagagactt tttacgtttg taaaccatca caaggagcag gacagtgctg acagctgcct
     cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg
     tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat
     agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     cttcaagaa
//