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ID   PKO8       preliminary; circular DNA; SYN; 3843 BP.
XX
AC   IG0081;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pKO-8 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pBR322-Tc from pBR322 & trp promoter]
RC   pKO-1 from pBR322-Tc & galK gene
RC   pKG1800 from pKO-1 & gal operon promoter
RC   pKG1900 from pKO-1 & gal operon promoter
RC   [lambda gal8 from lambda & gal operon promoter]
RC   pKB-2000 from pKO-1 & lambda gal8
RC   pKO-3, pKO-4, pKO-6, pKO-11 from pKO-1
RA   McKenney K., Shimatake H., Court D., Schmeissner U., Brady C.,
RA   Rosenberg M.;
RT   "A system to study promoter and terminator signals recognized by
RT   Escherichia coli RNA polymerase";
RL   Gene Amplif. Anal. 2:383-415(1981).
RL   Chirikjian J.G., Papas T.J.;
RL   (eds.), Elsevier, North Holland.
XX
CC   NM (pKO-8)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli GALK-)(bacteria GalE-T-K-)
CC   CP ()
CC   FN (promoter analysis)
CC   SE (color red/white)
CC   PA (pKO-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKO-1 EcoRI 3980bp 3979..3979
FT                   SalI linker 6bp gtcgac
FT                   -> pKO-8 3843bp"
FT   CDS             0..0
FT                   /note="GEN lambda O gene"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   CDS             468..1614
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   rep_origin      2080..2080
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2845..3705)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3843 BP; 961 A; 988 C; 993 G; 901 T; 0 other;
     cagagcgcca gcaagtgggg gacagcagaa gacctgaccg ccgcagagtg gatgtttgac
     atggtgaaga ctatcgcacc atcagccaga aaaccgattt tgctgggtgg gctaacgata
     tccgcctgat gcgtgaacgt gacggacgta accaccgcga catgtgtgtg ctgttccgct
     gggcatgcca ggacaacttc tggtccggta acgtgctgag cccggccaag cttactcccc
     atccccgggc aataagggct gcacgcgcac ttttatccgc ctctgctgcg ctccgccacc
     gtacgtaaat ttatggttgg ttatgaaatg ctggcagaga cccagcgaga cctgacagtc
     agcgatatcc attttcgcga atccggagtg taagaaatga gtctgaaaga aaaaacacaa
     tctctgtttg ccaacgcatt tggctaccct gccactcaca ccattcaggc gcctggccgc
     gtgaatttga ttggtgaaca caccgactac aacgacggtt tcgttctgcc ctgcgcgatt
     gattatcaaa ccgtgatcag ttgtgcacca cgcgatgacc gtaaagttcg cgtgatggca
     gccgattatg aaaatcagct cgacgagttt tccctcgatg cgcccattgt cgcacatgaa
     aactatcaat gggctaacta cgttcgtggc gtggtgaaac atctgcaact gcgtaacaac
     agcttcggcg gcgtggacat ggtgatcagc ggcaatgtgc cgcagggtgc cgggttaagt
     tcttccgctt cactggaagt cgcggtcgga accgtattgc agcagcttta tcatctgccg
     ctggacggcg cacaaatcgc gcttaacggt caggaagcag aaaaccagtt tgtaggctgt
     aactgcggga tcatggatca gctaatttcc gcgctcggca agaaagatca tgccttgctg
     atcgattgcc gctcactggg gaccaaagca gtttccatgc ccaaaggtgt ggctgtcgtc
     atcatcaaca gtaacttcaa acgtaccctg gttggcagcg aatacaacac ccgtcgtgaa
     cagtgcgaaa ccggtgcgcg tttcttccag cagccagccc tgcgtgatgt caccattgaa
     gagttcaacg ctgttgcgca tgaactggac ccgatcgtgg caaaacgcgt gcgtcatata
     ctgactgaaa acgcccgcac cgttgaagct gccagcgcgc tggagcaagg cgacctgaaa
     cgtatgggcg agttgatggc ggagtctcat gcctctatgc gcgatgattt cgaaatcacc
     gtgccgcaaa ttgacactct ggtagaaatc gtcaaagctg tgattggcga caaaggtggc
     gtacgcatga ccggcggcgg atttggcggc tgtatcgtcg cgctgatccc ggaagagctg
     gtgcctgccg tacagcaagc tgtcgctgaa caatatgaag caaaaacagg tattaaagag
     actttttacg tttgtaaacc atcacaagga gcaggacagt gctgacagct gcctcgcgcg
     tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     gaa
//