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ID   PKO9       preliminary; circular DNA; SYN; 3846 BP.
XX
AC   IG0082; ATCC37296;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKO-9 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pKO-9 from M13mp7 & pKO-4
RC   pKO-11 from pUC9 & pKO-1
RC   pCAP1P2 from pKO-9 & ilv gene promoter
RC   pCAP2 from pKO-9 & P2 gene promoter
RC   pCAP1 from pKO-11 & P1 gene promoter
RC   pCA1100 from pKO-1
RA   Adams C.W., Hatfield G.W.;
RT   "Effects of promoter strengths and growth conditions on copy number
RT   of transcription-fusion vectors";
RL   J. Biol. Chem. 259:7399-7403(1984).
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CC   This is a promoter cloning vector using expression of the promoterless
CC   galK gene for positive or color (MacConkey galactose indicator plates)
CC   selection. [4]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKO-9)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli GALK-)(bacteria GalE-T-K-)(E.coli JA221)(E.coli)
CC   CP ()
CC   FN (promoter analysis)
CC   SE (color red/white)
CC   PA (pKO-1)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKO-1 SmaI-EcoRI 3667bp 312..3979
FT                   fill in:
FT                   2. pUC9 BamHI-SmaI-EcoRI 10bp 254..264, MCS
FT                   fill in:
FT                   -> pKO-9 3846bp"
FT   CDS             0..0
FT                   /note="GEN lambda O gene"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   CDS             473..1619
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   terminator      0..0
FT                   /note="TER stop codon"
FT   rep_origin      2085..2085
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2850..3710)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
XX
SQ   Sequence 3846 BP; 960 A; 988 C; 994 G; 904 T; 0 other;
     cagcaagtgg gggacagcag aagacctgac cgccgcagag tggatgtttg acatggtgaa
     gactatcgca ccatcagcca gaaaaccgat tttgctgggt gggctaacga tatccgcctg
     atgcgtgaac gtgacggacg taaccaccgc gacatgtgtg tgctgttccg ctgggcatgc
     caggacaact tctggtccgg taacgtgctg agcccggcca agcttactcc ccatccccgg
     gatccgaatt cgggcaataa gggctgcacg cgcactttta tccgcctctg ctgcgctccg
     ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga
     cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa
     cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt caggcgcctg
     gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt ctgccctgcg
     cgattgatta tcaaaccgtg atcagttgtg caccacgcga tgaccgtaaa gttcgcgtga
     tggcagccga ttatgaaaat cagctcgacg agttttccct cgatgcgccc attgtcgcac
     atgaaaacta tcaatgggct aactacgttc gtggcgtggt gaaacatctg caactgcgta
     acaacagctt cggcggcgtg gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt
     taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc
     tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac cagtttgtag
     gctgtaactg cgggatcatg gatcagctaa tttccgcgct cggcaagaaa gatcatgcct
     tgctgatcga ttgccgctca ctggggacca aagcagtttc catgcccaaa ggtgtggctg
     tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac aacacccgtc
     gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agccctgcgt gatgtcacca
     ttgaagagtt caacgctgtt gcgcatgaac tggacccgat cgtggcaaaa cgcgtgcgtc
     atatactgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag caaggcgacc
     tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat gatttcgaaa
     tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agctgtgatt ggcgacaaag
     gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg atcccggaag
     agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa acaggtatta
     aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga cagctgcctc
     gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     ttcaag
//