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ID   PKOTW1     preliminary; circular DNA; SYN; 3939 BP.
XX
AC   ATCC37297;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKOTW1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pBR322-Tc from pBR322 & trp promoter]
RC   pKO-1 from pBR322-Tc & galK gene
RC   pKG1800 from pKO-1 & gal operon promoter
RC   pKG1900 from pKO-1 & gal operon promoter
RC   [lambda gal8 from lambda & gal operon promoter]
RC   pKB-2000 from pKO-1 & lambda gal8
RC   pKO-4, pKO-6, pKO-11 from pKO-1
RA   McKenney K., Shimatake H., Court D., Schmeissner U., Brady C.,
RA   Rosenberg M.;
RT   "A system to study promoter and terminator signals recognized by
RT   Escherichia coli RNA polymerase";
RL   Gene Amplif. Anal. 2:383-415(1981).
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RN   [2]
RC   pKO-9 from M13mp7 & pKO-4
RC   pKO-11 from pUC9 & pKO-1
RC   pCAP1P2 from pKO-9 & ilv gene promoter
RC   pCAP2 from pKO-9 & P2 gene promoter
RC   pCAP1 from pKO-11 & P1 gene promoter
RC   pCA1100 from pKO-1
RA   Adams C.W., Hatfield G.W.;
RT   "Effects of promoter strengths and growth conditions on copy
RT   number of transcription-fusion vectors";
RL   J. Biol. Chem. 259:7399-7403(1984).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   This is a promoter cloning vector using expression of the promoterless
CC   galK gene for positive or color (MacConkey galactose indicator plates)
CC   selection.
CC   pKOTW1 differs from pKO11 (ATCC 37122) in a deletion of the EcoRI site
CC   located at 4002 bp in pKO11.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKOTW1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JA221)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pKO-11)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pKO-11 EcoRI 3935bp 263..263
FT                   fill in
FT                   -> pKOTW1 4010bp"
FT   -               1..266
FT                   /note="pKO-11 1..266 266bp
FT                   EcoRI = G^AATT C
FT                   EcoRI =      G^AATTC"
FT   -               267..3939
FT                   /note="pKO-11 263..3935 3673bp"
FT   misc_binding    0..0
FT                   /note="MCS NruI-EcoRI-AvaI-SmaI-XmaI-BamHI-SalI-
FT                   HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique NruI-EcoRI-AvaI-SmaI-XmaI-BamHI-
FT                   SalI-HindIII-SphI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN E. coli galactokinase gene (galK);
FT                   reporter gene"
XX
SQ   Sequence 3939 BP; 979 A; 1013 C; 1018 G; 920 T; 9 other;
     gccagcaagt gggggacagc agaagacctg accgccgcag agtggatgtt tgacatggtg
     aagactatcg caccatcagc cagaaaaccg attttgctgg gtgggctaac gatatccgcc
     tgatgcgtga acgtgacgga cgtaaccacc gcgacatgtg tgtgctgttc cgctgggcat
     gccaggacaa cttctggtcc ggtaacgtgc tgagcccggc caagcttact ccccatcccc
     ctgcagtcga cggatcccgg ggaattaatt cgggcaataa gggctgcacg cgcactttta
     tccgcctctg ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc
     agagacccag cgagacctga cagtcagcga tatccatttt cgcgaatccg gagtgtaaga
     aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac
     tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga
     cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagttgtg caccacgcga
     tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct
     cgatgcgccc attgtcgcac atgaaaacta tcaatgggct aactacgttc gtggcgtggt
     gaaacatctg caactgcgta acaacagctt cggcggcgtg gacatggtga tcagcggcaa
     tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt
     attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga
     agcagaaaac cagtttgtag gctgtaactg cgggatcatg gatcagctaa tttccgcgct
     cggcaagaaa gatcatgcct tgctgatcga ttgccgctca ctggggacca aagcagtttc
     catgcccaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg
     cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc
     agccctgcgt gatgtcacca ttgaagagtt caacgctgtt gcgcatgaac tggacccgat
     cgtggcaaaa cgcgtgcgtc atatactgac tgaaaacgcc cgcaccgttg aagctgccag
     cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc
     tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa
     agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat
     cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata
     tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg
     acagtgctga cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     agctcccgga gannnnnnnn ncagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//