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ID   PKP497     preliminary; circular DNA; SYN; 2905 BP.
XX
AC   M74187;
XX
DT   18-FEB-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKP497 - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-2905
RC   pKP497 from pUC19
RA   Rondahl H., Nilsson B., Holmgren E.;
RT   "Fusions to the 5' end of a gene encoding a two-domain analogue of
RT   staphylococcal protein A";
RL   Unpublished (1991).
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RN   [2]
RC   pKP497
RA   Holmgren E.;
RT   ;
RL   Submitted (18-FEB-1992) to GenBank by:
RL   Holmgren E., Department of Microbiology, Dabi Pharmacia, KabiGen,
RL   Strandbergsgaten 49, S-11287 Stockholm, SWEDEN.
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CC   NM (pKP497)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(screening)(expression)(fusion)
CC   SE ()
CC   PA (pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   2. oligo 219bp, MCS/ZZ
FT                   -> pKP497 2905bp"
FT   misc_feature    1..54
FT                   /note="from plasmid pUC19"
FT   misc_recomb     54..55
FT                   /note="pUC19 plasmid DNA end/synthetic polylinker I
FT                   start"
FT   misc_feature    55..110
FT                   /note="MCS synthetic polylinker I"
FT   misc_recomb     110..111
FT                   /note="synthetic polylinker I end/synthetic ZZ gene
FT                   DNA start"
FT   CDS             111..758
FT                   /note="GEN synthetic ZZ gene (protein A analogue)
FT                   and E. coli beta-galactosidase gene (lacZ')
FT                   IgG-binding fusion gene (ZZ-lacZ'); putative;
FT                   suppressible stop codon TAG at bases 489..491;
FT                   synthetic ZZ from bases 110..488;
FT                   lacZ' of pUC19 from bases 492..755"
FT   mat_peptide     111..488
FT                   /note="ZZ-lacZ' peptide with two IgG-binding domains"
FT   misc_recomb     488..489
FT                   /note="synthetic ZZ gene DNA end/synthetic polylinker
FT                   II start"
FT   misc_feature    489..502
FT                   /note="TER suppressible stop codon TAG at bases
FT                   489..491; synthetic polylinker II"
FT   misc_feature    492..502
FT                   /note="MCS synthetic polylinker II"
FT   mat_peptide     492..755
FT                   /note="E. coli lacZ' mature peptide (in ZZ-lacZ');
FT                   bases 492..501 of lacZ' from polylinker II;
FT                   bases 502..755 of lacZ' from pUC19"
FT   misc_recomb     502..503
FT                   /note="synthetic polylinker II end/plasmid pUC19 DNA
FT                   start"
FT   misc_feature    503..2905
FT                   /note="from plasmid pUC19"
XX
SQ   Sequence 2905 BP; 776 A; 736 C; 700 G; 693 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggagaca
     gctgagcggc cgccaagctt gcatgcctgc aggtcgactc tagaggatcc cggcaacacg
     atgaagccgt agacaacaaa ttcaacaaag aacaacaaaa cgcgttctat gagatcttac
     atttacctaa cttaaacgaa gaacaacgaa acgccttcat ccaaagttta aaagatgacc
     caagccaaag cgctaacctt ttagcagaag ctaaaaagct aaatgatgct caggcgccga
     aagtagacaa caaattcaac aaagaacaac aaaacgcgtt ctatgagatc ttacatttac
     ctaacttaaa cgaagaacaa cgaaacgcct tcatccaaag tttaaaagat gacccaagcc
     aaagcgctaa ccttttagca gaagctaaaa agctaaatga tgctcaggcg ccgaaagtag
     acgcgaatta ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg
     aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     agtcagtgag cgaggaagcg gaaga
//