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ID   PKTH604    preliminary; circular DNA; SYN; 4038 BP.
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AC   L08926; VB0103; K00895; K00896; K00897; ATCC37227;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKTH604 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pKTH601, pKTH604, pKTH605, pKTH606 from pBR322 & oligo
RC   pKTH38, pKTH51, pKTH73 from pBD9 & pUB110 & pE194
RC   pKTH95 from pBR325 & pKTH73 & pKTH38
RC   pKTH96 from pKTH10 & pKTH73 & pKTH51
RC   pKTH97 from pKTH96
RC   pKTH99 from pKTH95
RC   pKTH115 from pKTH99 & pKTH606
RC   pKTH114 from pKTH97 & pKTH601
RA   Pettersson R.F., Lundstrom K., Chattopadhyaya J.B., Josephson S.,
RA   Philipson L., Kaariainen L., Palva I.;
RT   "Chemical synthesis and molecular cloning of a STOP oligonucleotide
RT   encoding an UGA translation terminator in all three reading frames";
RL   Gene 24:15-27(1983).
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RN   [2]
RP   1-4038
RC   pKTH604
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled according to the cloning strategy given in ref 1 by F.
CC   Pfeiffer.
CC   The STOP oligonucleotide TGATTGATTGA contains STOP codons in all
CC   three reading frames. It was inserted into pBR322 in different
CC   configurations:
CC   vector  orien-      pBR322 flank     sequence      flank pBR322
CC           tation       site                                 site
CC   pKTH601 (forw)     HindIII gc       TGATTGATTGA       cc BamHI
CC   pKTH604 ( rev)     HindIII gc       TCAATCAATCA       cc BamHI
CC   pKTH605 (forw-rev) HindIII gc TGATTGATTGA-TCAATCAATCA cc BamHI
CC   pKTH606 (forw)       EcoRI  c       TGATTGATTGA       cc BamHI
CC
CC   The depositor has requested that any proposed commercial use of the
CC   cells be negotiated with him.  The sending of these cultures does not
CC   and should not be construed to grant any license or rights to the
CC   materials to the recipient. (ATCC staff)
CC   No distribution of the cultures or any derivatives therefrom should
CC   be made to third parties. (ATCC staff)
CC   Vector containing a portable translation stop codon
CC   (BamHI/stop/HindIII).
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKTH604)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(SFVE1STOP)
CC   BR (pKTH601)(pKTH605)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove HindIII-BamHI 346bp 30..376,
FT                   \ 4015bp
FT                   2. oligo BamHI/3-frame stop codon/HindIII 11bp
FT                   \ tgattgattga
FT                   -> pKTH604 4038bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    1..34
FT                   /note="from pBR322"
FT   misc_feature    complement(37..47)
FT                   /note="STOP-oligonucleotide"
FT   misc_feature    50..4038
FT                   /note="from pBR322"
FT   CDS             complement(2973..3761)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 4038 BP; 925 A; 1111 C; 1051 G; 951 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gcttgctcaa tcaatcaccg gatcctctac
     gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
     gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
     ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
     gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
     atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt caacccagtc
     agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc
     tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc
     ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt
     atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc
     tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg
     caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc
     gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc gatttatgcc
     gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc
     tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc
     ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga
     gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc gccatctcca
     gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
     tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
     aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
     caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
     cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
     cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
     gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
     catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
     acagaaattc ccccttacac ggaggcatca agtgaccaaa caggaaaaaa ccgcccttaa
     catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
     cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
     cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     ccctttcgtc ttcaagaa
//