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ID   PKTH605    preliminary; circular DNA; SYN; 4049 BP.
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AC   L08927; VB0104; K00895; K00896; K00897; ATCC37228;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKTH605 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pKTH601, pKTH604, pKTH605, pKTH606 from pBR322 & oligo
RC   pKTH38, pKTH51, pKTH73 from pBD9 & pUB110 & pE194
RC   pKTH95 from pBR325 & pKTH73 & pKTH38
RC   pKTH96 from pKTH10 & pKTH73 & pKTH51
RC   pKTH97 from pKTH96
RC   pKTH99 from pKTH95
RC   pKTH115 from pKTH99 & pKTH606
RC   pKTH114 from pKTH97 & pKTH601
RA   Pettersson R.F., Lundstrom K., Chattopadhyaya J.B., Josephson S.,
RA   Philipson L., Kaariainen L., Palva I.;
RT   "Chemical synthesis and molecular cloning of a STOP oligonucleotide
RT   encoding an UGA translation terminator in all three reading frames";
RL   Gene 24:15-27(1983).
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RN   [2]
RP   1-4049
RC   pKTH605
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled according to the cloning strategy given in ref 1 by F.
CC   Pfeiffer.
CC   The STOP oligonucleotide TGATTGATTGA contains STOP codons in all
CC   three reading frames. It was inserted into pBR322 in different
CC   configurations:
CC   vector  orien-      pBR322 flank     sequence      flank pBR322
CC           tation       site                                 site
CC   pKTH601 (forw)     HindIII gc       TGATTGATTGA       cc BamHI
CC   pKTH604 ( rev)     HindIII gc       TCAATCAATCA       cc BamHI
CC   pKTH605 (forw-rev) HindIII gc TGATTGATTGA-TCAATCAATCA cc BamHI
CC   pKTH606 (forw)       EcoRI  c       TGATTGATTGA       cc BamHI
CC
CC   Vector containing portable translation stop codons in two oriendations
CC   (HindIII/stop/pots/BamHI).
CC   The depositor has requested that any proposed commercial use of the
CC   cells be negotiated with him.  The sending of these cultures does not
CC   and should not be construed to grant any license or rights to the
CC   materials to the recipient. (ATCC staff)
CC   No distribution of the cultures or any derivatives therefrom should
CC   be made to third parties. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKTH605)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(SFVE1STOP)
CC   BR (pKTH601)(pKTH604)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove HindIII-BamHI 346bp 30..376,
FT                   \ 4015bp
FT                   2. oligo HindIII/3 stop/reversed/BamHI 11bp
FT                   \ tgattgattga
FT                   -> pKTH605 4049bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    1..34
FT                   /note="from pBR322"
FT   misc_feature    37..47
FT                   /note="STOP-oligonucleotide"
FT   misc_feature    complement(48..58)
FT                   /note="STOP-oligonucleotide"
FT   misc_feature    61..4049
FT                   /note="from pBR322"
FT   CDS             complement(2984..3772)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 4049 BP; 928 A; 1111 C; 1054 G; 956 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gcttgctgat tgattgatca atcaatcacc
     ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct
     tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg
     cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
     tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
     gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
     attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc
     cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt
     gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
     ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
     tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
     gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct
     accctgtgga acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt
     tctctggtcc cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg
     ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat
     tacccccatg aacagaaatt cccccttaca cggaggcatc aagtgaccaa acaggaaaaa
     accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc
     aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat
     gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     gtatcacgag gccctttcgt cttcaagaa
//