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ID   PKTH606    preliminary; circular DNA; SYN; 4006 BP.
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AC   L08928; VB0105; K00895; K00896; K00897; ATCC37229;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pKTH606 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pKTH601, pKTH604, pKTH605, pKTH606 from pBR322 & oligo
RC   pKTH38, pKTH51, pKTH73 from pBD9 & pUB110 & pE194
RC   pKTH95 from pBR325 & pKTH73 & pKTH38
RC   pKTH96 from pKTH10 & pKTH73 & pKTH51
RC   pKTH97 from pKTH96
RC   pKTH99 from pKTH95
RC   pKTH115 from pKTH99 & pKTH606
RC   pKTH114 from pKTH97 & pKTH601
RA   Pettersson R.F., Lundstrom K., Chattopadhyaya J.B., Josephson S.,
RA   Philipson L., Kaariainen L., Palva I.;
RT   "Chemical synthesis and molecular cloning of a STOP oligonucleotide
RT   encoding an UGA translation terminator in all three reading frames";
RL   Gene 24:15-27(1983).
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RN   [2]
RP   1-4006
RC   pKTH606
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Assembled according to the cloning strategy given in ref 1 by F.
CC   Pfeiffer.
CC   The STOP oligonucleotide TGATTGATTGA contains STOP codons in all
CC   three reading frames. It was inserted into pBR322 in different
CC   configurations:
CC   vector  orien-      pBR322 flank     sequence      flank pBR322
CC           tation       site                                 site
CC   pKTH601 (forw)     HindIII gc       TGATTGATTGA       cc BamHI
CC   pKTH604 ( rev)     HindIII gc       TCAATCAATCA       cc BamHI
CC   pKTH605 (forw-rev) HindIII gc TGATTGATTGA-TCAATCAATCA cc BamHI
CC   pKTH606 (forw)       EcoRI  c       TGATTGATTGA       cc BamHI
CC
CC   Vector containing a portable translation stop codon
CC   (EcoRI-stop-BamHI).
CC   The depositor has requested that any proposed commercial use of the
CC   cells be negotiated with him.  The sending of these cultures does not
CC   and should not be construed to grant any license or rights to the
CC   materials to the recipient. (ATCC staff)
CC   No distribution of the cultures or any derivatives therefrom should
CC   be made to third parties. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pKTH606)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(SFVE1STOP)
CC   BR (pKTH601)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-BamHI 377bp
FT                   \ 4360..4361..376, 3984bp
FT                   2. oligo EcoRI/3-frame stop codon/BamHI 11bp
FT                   \ tgattgattga
FT                   -> pKTH606 4006bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-BamHI-PstI"
FT   misc_feature    1..3
FT                   /note="from pBR322"
FT   misc_feature    5..15
FT                   /note="TER STOP-oligonucleotide"
FT   misc_feature    18..4006
FT                   /note="from pBR322"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2941..3729)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 4006 BP; 915 A; 1102 C; 1048 G; 941 T; 0 other;
     ttcctgattg attgaccgga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc
     gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg
     accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag
     aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt
     gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg
     cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac
     tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt
     tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc
     gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat
     tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca
     caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct
     cgtttcatcg gtatcattac ccccatgaac agaaattccc ccttacacgg aggcatcaag
     tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa
     cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc
     ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg
     aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaa
//