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ID   PKUN9A     preliminary; circular DNA; SYN; 3902 BP.
XX
AC   M13163; A14395; A14396; A14397;
XX
DT   16-JUL-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pKUN9 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1951-2030
RC   phage IKe from N-specific ss-DNA phage
RC   phage IKe-9 from phage IKe
RC   IR1 from phage f1
RC   pUCI-2e from pUC9 & pIKori-2b(1)
RC   pKUN9 from pUCI-2e & pEMBL8+
RC   M13, phage fd, phage f1 from F-specific ss-DNA phages
RA   Peeters B.P.H., Schoenmakers J.G., Konings R.N.H.;
RT   "Plasmid pKUN9, a versatile vector for the selective packaging of
RT   both DNA strands into single-stranded DNA-containing phage-like
RT   particles";
RL   Gene 41:39-46(1986).
XX
RN   [2]
RC   IR1 from phage f1
RC   phage IKe from E. coli
RC   pBP9 from pUR222 & M13mp9
RC   pIK200 from pBP9 & phage IKe
RC   pIK203 from pIK200
RC   pIK001 from pIK200, IKe ori
RC   pIKori-1, pIKori-2, pIKori-5, pIKori-7 from pIK001
RC   pIKori-2b(1), pIKori-2b(23) from pIKori-2
RA   Peeters B.P.H., Schoenmakers J.G., Konings R.N.H.;
RT   "The gene II proteins of the filamentous phages IKe and Ff (M13,
RT   fd and f1) are not functionally interchangeable during viral
RT   strand replication";
RL   Nucleic Acids Res. 14:5067-5080(1986).
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RN   [3]
RP   1-3902
RC   pKUN9
RA   Konings R.N.H.;
RT   ;
RL   Submitted (02-MAR-1987) to GenBank on paper by:
RL   Konings R.N.H., Univ. of Nijmegen, Nijmegen, The Netherlands.
XX
RN   [4]
RC   phage IKe from E.coli
RA   Peeters B.P., Peters R.M., Schoenmakers J.G., Konings R.N.;
RT   "Nucleotide sequence and genetic organization of the genome of the
RT   N-specific filamentous bacteriophage IKe. Comparison with the
RT   genome of the F-specific filamentous phages M13, fd and f1";
RL   J. Mol. Biol. 181:27-39(1985).
XX
CC   NCBI gi: 208894
CC   NM (pKUN9)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (packaging both strands)
CC   SE (color blue/white)
CC   PA (pUC9)(IKe)(f1)
CC   BR (pKUN9B)
CC   OF ()
CC   OR (2000 bp upstream of HindIII site)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli
FT                   -> phage IKe
FT                   1. pUR222 remove PvuII-PvuII 295bp 1742..2037,
FT                   \ MCS/2405bp
FT                   2. M13mp9 PvuII-PvuII 463bp 5963..6426, MCS
FT                   -> pBP9 2868bp [unique SmaI, unique HindIII]
FT                   1. pBP9 SmaI 2868bp
FT                   2. phage IKe HincII-HincII, ori/gene II/HincII A frag
FT                   -> pIK200
FT                   1. pIK200 BamHI-BglII, gene II and promoter
FT                   -> pIK203
FT                   1. pIK200 HincII-EcoRI, IKe ori
FT                   -> pIK001
FT                   1. phage IKe AluI-AluI, IKe ori
FT                   2. pBP9 AluI
FT                   -> pIKori-2
FT                   1. pIKori-2
FT                   BAL31 nuclease, remove part of IKe ori
FT                   -> pIKori-2b(1) 3400bp
FT                   1. pUC9 NdeI 2665bp 478..478, MCS
FT                   2. pIKori-2b(1) EcoRI-BamHI, IKe ori/morphogenetic sig
FT                   Klenow:Klenow
FT                   -> pUCI-2e
FT                   1. pUCI-2e NarI
FT                   Klenow
FT                   2. pEMBL8+ RsaI-RsaI 514bp 771..1285, f1 ori
FT                   -> pKUN9 3902bp"
FT   CDS             complement(1..137)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_recomb     530..531
FT                   /note="pUC9 DNA end/IKe DNA start"
FT   misc_recomb     1235..1236
FT                   /note="IKe DNA end/pUC9 DNA start"
FT   misc_recomb     1296..1297
FT                   /note="pUC9 DNA end/f1 DNA start"
FT   CDS             complement(1744..2022)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   misc_recomb     1810..1811
FT                   /note="f1 DNA end/pUC9 DNA start"
FT   CDS             complement(3179..3902)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3902 BP; 989 A; 988 C; 961 G; 964 T; 0 other;
     tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     aactatgcgg catcagagca gattgtactg agagtgcacc ataaattccc ttgaaggaga
     gagggctgta gcacgttgca aaatgcaaca agagtccact attaaacata gtggactcac
     cacgaccgag ccgactgcca gatccaaaat cgccgcttcc acgtcctttc gtctctacga
     ccgtcaaact acaaaatcgc ttatcgatca ccaaaagaaa aaaacgtcta acaaggcgat
     ggcccactac gtgaaccatc acccataaca aggatattta tggagtgtcg taaatcacta
     aatcggccca aaggggcccc cacgatttag cgtttgacga ttcgacgccg gagaacgccg
     aaagagaagg gcggagtacg tgaaaattat cacgtactaa ctagccgtta gcaccgcagc
     cattatggat gtgaaaaccg aggaacatac gttgtatcga agccgcgcac acggagaggc
     gcaagagtct ctacgggcag ggctagcgca cctacccggc agcctcaaag cgccttaata
     acgtgcgcgg taaggagaac aaacatagtt ctttcgttat gcgaatcact atggctgcgg
     aacaaccagc caatcacagg tacgctttca aaccaaggta cagagcgatt accgtcagtt
     gtattggaat cgataagacc accaagcaaa agcgtctgac cgtcttttat ctgcactgtc
     gtttgcatat gacgctgatt agtaatgatg tcgctgggga tctatgcggt gtgaaatacc
     gcacagatgc gtaaggagaa aataccgcat caggcgacgc gccctgtagc ggcgcattaa
     gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     cgtttacaat ttaaatattt gcttatacaa tcttcctgtt tttggggctt ttctgattat
     caaccggggt cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcccggg
     gatccgtcga cctgcagcca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
     cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     gt
//