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ID PKUN9A preliminary; circular DNA; SYN; 3902 BP.
XX
AC M13163; A14395; A14396; A14397;
XX
DT 16-JUL-1988 (Rel. 3, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pKUN9 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1951-2030
RC phage IKe from N-specific ss-DNA phage
RC phage IKe-9 from phage IKe
RC IR1 from phage f1
RC pUCI-2e from pUC9 & pIKori-2b(1)
RC pKUN9 from pUCI-2e & pEMBL8+
RC M13, phage fd, phage f1 from F-specific ss-DNA phages
RA Peeters B.P.H., Schoenmakers J.G., Konings R.N.H.;
RT "Plasmid pKUN9, a versatile vector for the selective packaging of
RT both DNA strands into single-stranded DNA-containing phage-like
RT particles";
RL Gene 41:39-46(1986).
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RN [2]
RC IR1 from phage f1
RC phage IKe from E. coli
RC pBP9 from pUR222 & M13mp9
RC pIK200 from pBP9 & phage IKe
RC pIK203 from pIK200
RC pIK001 from pIK200, IKe ori
RC pIKori-1, pIKori-2, pIKori-5, pIKori-7 from pIK001
RC pIKori-2b(1), pIKori-2b(23) from pIKori-2
RA Peeters B.P.H., Schoenmakers J.G., Konings R.N.H.;
RT "The gene II proteins of the filamentous phages IKe and Ff (M13,
RT fd and f1) are not functionally interchangeable during viral
RT strand replication";
RL Nucleic Acids Res. 14:5067-5080(1986).
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RN [3]
RP 1-3902
RC pKUN9
RA Konings R.N.H.;
RT ;
RL Submitted (02-MAR-1987) to GenBank on paper by:
RL Konings R.N.H., Univ. of Nijmegen, Nijmegen, The Netherlands.
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RN [4]
RC phage IKe from E.coli
RA Peeters B.P., Peters R.M., Schoenmakers J.G., Konings R.N.;
RT "Nucleotide sequence and genetic organization of the genome of the
RT N-specific filamentous bacteriophage IKe. Comparison with the
RT genome of the F-specific filamentous phages M13, fd and f1";
RL J. Mol. Biol. 181:27-39(1985).
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CC NCBI gi: 208894
CC NM (pKUN9)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (packaging both strands)
CC SE (color blue/white)
CC PA (pUC9)(IKe)(f1)
CC BR (pKUN9B)
CC OF ()
CC OR (2000 bp upstream of HindIII site)
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. E. coli
FT -> phage IKe
FT 1. pUR222 remove PvuII-PvuII 295bp 1742..2037,
FT \ MCS/2405bp
FT 2. M13mp9 PvuII-PvuII 463bp 5963..6426, MCS
FT -> pBP9 2868bp [unique SmaI, unique HindIII]
FT 1. pBP9 SmaI 2868bp
FT 2. phage IKe HincII-HincII, ori/gene II/HincII A frag
FT -> pIK200
FT 1. pIK200 BamHI-BglII, gene II and promoter
FT -> pIK203
FT 1. pIK200 HincII-EcoRI, IKe ori
FT -> pIK001
FT 1. phage IKe AluI-AluI, IKe ori
FT 2. pBP9 AluI
FT -> pIKori-2
FT 1. pIKori-2
FT BAL31 nuclease, remove part of IKe ori
FT -> pIKori-2b(1) 3400bp
FT 1. pUC9 NdeI 2665bp 478..478, MCS
FT 2. pIKori-2b(1) EcoRI-BamHI, IKe ori/morphogenetic sig
FT Klenow:Klenow
FT -> pUCI-2e
FT 1. pUCI-2e NarI
FT Klenow
FT 2. pEMBL8+ RsaI-RsaI 514bp 771..1285, f1 ori
FT -> pKUN9 3902bp"
FT CDS complement(1..137)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_recomb 530..531
FT /note="pUC9 DNA end/IKe DNA start"
FT misc_recomb 1235..1236
FT /note="IKe DNA end/pUC9 DNA start"
FT misc_recomb 1296..1297
FT /note="pUC9 DNA end/f1 DNA start"
FT CDS complement(1744..2022)
FT /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT misc_recomb 1810..1811
FT /note="f1 DNA end/pUC9 DNA start"
FT CDS complement(3179..3902)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
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SQ Sequence 3902 BP; 989 A; 988 C; 961 G; 964 T; 0 other;
tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
aactatgcgg catcagagca gattgtactg agagtgcacc ataaattccc ttgaaggaga
gagggctgta gcacgttgca aaatgcaaca agagtccact attaaacata gtggactcac
cacgaccgag ccgactgcca gatccaaaat cgccgcttcc acgtcctttc gtctctacga
ccgtcaaact acaaaatcgc ttatcgatca ccaaaagaaa aaaacgtcta acaaggcgat
ggcccactac gtgaaccatc acccataaca aggatattta tggagtgtcg taaatcacta
aatcggccca aaggggcccc cacgatttag cgtttgacga ttcgacgccg gagaacgccg
aaagagaagg gcggagtacg tgaaaattat cacgtactaa ctagccgtta gcaccgcagc
cattatggat gtgaaaaccg aggaacatac gttgtatcga agccgcgcac acggagaggc
gcaagagtct ctacgggcag ggctagcgca cctacccggc agcctcaaag cgccttaata
acgtgcgcgg taaggagaac aaacatagtt ctttcgttat gcgaatcact atggctgcgg
aacaaccagc caatcacagg tacgctttca aaccaaggta cagagcgatt accgtcagtt
gtattggaat cgataagacc accaagcaaa agcgtctgac cgtcttttat ctgcactgtc
gtttgcatat gacgctgatt agtaatgatg tcgctgggga tctatgcggt gtgaaatacc
gcacagatgc gtaaggagaa aataccgcat caggcgacgc gccctgtagc ggcgcattaa
gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
cgtttacaat ttaaatattt gcttatacaa tcttcctgtt tttggggctt ttctgattat
caaccggggt cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcccggg
gatccgtcga cctgcagcca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
gt
//