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ID   PKUN9B     preliminary; circular DNA; SYN; 3923 BP.
XX
AC   M19265; A14395; A14396; A14397;
XX
DT   15-JUN-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pKUN9 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1951-2030
RC   phage IKe from N-specific ss-DNA phage
RC   phage IKe-9 from phage IKe
RC   IR1 from phage f1
RC   pUCI-2e from pUC9 & pIKori-2b(1)
RC   pKUN9 from pUCI-2e & pEMBL8+
RC   M13, phage fd, phage f1 from F-specific ss-DNA phages
RA   Peeters B.P.H., Schoenmakers J.G., Konings R.N.H.;
RT   "Plasmid pKUN9, a versatile vector for the selective packaging of
RT   both DNA strands into single-stranded DNA-containing phage-like
RT   particles";
RL   Gene 41:39-46(1986).
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RN   [2]
RP   1-3923
RC   pKUN9
RA   Konings R.N.H.;
RT   ;
RL   Submitted (02-MAR-1987) to GenBank on paper by:
RL   Konings R.N.H., Univ. of Nijmegen, Nijmegen, The Netherlands.
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CC   NCBI gi: 208897
CC   NM (pKUN9)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (packaging both strands)
CC   SE (color blue/white)
CC   PA (pUC9)(IKe)(f1)
CC   BR (pKUN9A)
CC   OF ()
CC   OR (2021 bp upstream of HindIII site)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli
FT                   -> phage IKe
FT                   1. pUR222 remove PvuII-PvuII 295bp 1742..2037,
FT                   \ MCS/2405bp
FT                   2. M13mp9 PvuII-PvuII 463bp 5963..6426, MCS
FT                   -> pBP9 2868bp [unique SmaI, unique HindIII]
FT                   1. pBP9 SmaI 2868bp
FT                   2. phage IKe HincII-HincII, ori/gene II/HincII A frag
FT                   -> pIK200
FT                   1. pIK200 BamHI-BglII, gene II and promoter
FT                   -> pIK203
FT                   1. pIK200 HincII-EcoRI, IKe ori
FT                   -> pIK001
FT                   1. phage IKe AluI-AluI, IKe ori
FT                   2. pBP9 AluI
FT                   -> pIKori-2
FT                   1. pIKori-2
FT                   BAL31 nuclease, remove part of IKe ori
FT                   -> pIKori-2b(1) 3400bp
FT                   1. pUC9 NdeI 2665bp 478..478, MCS
FT                   2. pIKori-2b(1) EcoRI-BamHI, IKe ori/morphogenetic sig
FT                   Klenow:Klenow
FT                   -> pUCI-2e
FT                   1. pUCI-2e NarI
FT                   Klenow
FT                   2. pEMBL8+ RsaI-RsaI 514bp 771..1285, f1 ori
FT                   -> pKUN9 3923bp"
FT   CDS             complement(1..137)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_recomb     530..531
FT                   /note="pUC9 DNA end/IKe DNA start"
FT   misc_recomb     1235..1236
FT                   /note="IKe DNA end/pUC9 DNA start"
FT   misc_recomb     1296..1297
FT                   /note="pUC9 DNA end/f1 DNA start"
FT   CDS             complement(1744..2043)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   misc_recomb     1810..1811
FT                   /note="f1 DNA end/pUC9 DNA start"
FT   CDS             complement(3200..3923)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3923 BP; 994 A; 992 C; 968 G; 969 T; 0 other;
     tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     aactatgcgg catcagagca gattgtactg agagtgcacc ataaattccc ttgaaggaga
     gagggctgta gcacgttgca aaatgcaaca agagtccact attaaacata gtggactcac
     cacgaccgag ccgactgcca gatccaaaat cgccgcttcc acgtcctttc gtctctacga
     ccgtcaaact acaaaatcgc ttatcgatca ccaaaagaaa aaaacgtcta acaaggcgat
     ggcccactac gtgaaccatc acccataaca aggatattta tggagtgtcg taaatcacta
     aatcggccca aaggggcccc cacgatttag cgtttgacga ttcgacgccg gagaacgccg
     aaagagaagg gcggagtacg tgaaaattat cacgtactaa ctagccgtta gcaccgcagc
     cattatggat gtgaaaaccg aggaacatac gttgtatcga agccgcgcac acggagaggc
     gcaagagtct ctacgggcag ggctagcgca cctacccggc agcctcaaag cgccttaata
     acgtgcgcgg taaggagaac aaacatagtt ctttcgttat gcgaatcact atggctgcgg
     aacaaccagc caatcacagg tacgctttca aaccaaggta cagagcgatt accgtcagtt
     gtattggaat cgataagacc accaagcaaa agcgtctgac cgtcttttat ctgcactgtc
     gtttgcatat gacgctgatt agtaatgatg tcgctgggga tctatgcggt gtgaaatacc
     gcacagatgc gtaaggagaa aataccgcat caggcgacgc gccctgtagc ggcgcattaa
     gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     cgtttacaat ttaaatattt gcttatacaa tcttcctgtt tttggggctt ttctgattat
     caaccggggt cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcgagct
     cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggcg taatcatggt
     catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct
     cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     tcttaccgct gttgagatcc agt
//