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ID   PL1        preliminary; circular DNA; SYN; 2586 BP.
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AC   ATCC37369;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pL1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pBR322SV from pBR322 & SV40 late polyA
RC   pcDV1 from pBR322SV
RC   pBR322-SV40 from pBR322 & SV40 early prom/splice acceptor/ori
RC   pL1 from pBR322-SV40
RC   [pcD-X from pcDV1]
RC   pcD-dhfr from pcDV1 & pL1 & DHFR gene
RC   pcD-alphaG from pcDV1 & pL1 & alpha-globin gene
RA   Okayama H., Berg P.;
RT   "A cDNA cloning vector that permits expression of cDNA inserts in
RT   mammalian cells";
RL   Mol. Cell. Biol. 3:280-289(1983).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   pcDV1 is an oligo dT-tailed plasmid primer. It is 3088 bp untailed.
CC   It works together with oligo dG-tailed pL1 linker, which has
CC   SV40 origin, SV40 early promoter, and SV40 mRNA splicing, and has
CC   a HindIII at one end and polyG at the PstI site at the other.
CC   pL1 linker has a BglI site in SV40 ori and an XhoI and BamHI site.
CC   A 16S splice junction is at the BamHI site.
CC   DNA ligase, RNaseH, and DNA polymerase I makes a dsDNA circle.
CC   cDNA synthesis is from polyA mRNA and is oriented,
CC   with linker 5' and polyA signal on plasmid 3' to cDNA.
CC   The SV40 segment contains KpnI and EcoRI sites for the preparation
CC   of vector-primer containing a poly(dT) tail at one end & the
CC   polyadenylation sequence from the late region of the virus.
CC   pcDV1 (ATCC 37368) and pL1 (ATCC 37369) should be used together to
CC   construct a cDNA library.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pL1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(SV40)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII-PvuII 2037bp 30..2067
FT                   2. SV40 HindIII-BamHI 825bp 1709..2534,
FT                   \ early prom/acc/ori/0.19 to 0.32
FT                   \ SV40 HindIII = 1047 1494 1709 3477 4003 5172
FT                   -> pBR322-SV40 2862bp
FT                   1. pBR322-SV40 PstI 2862bp,
FT                   \ SV40 HindIII = 1047 1494 1709 3477 4003 5172
FT                   \ SV40 PstI = 1993 3209
FT                   oligo dG tail
FT                   -> pL1 2600bp"
FT   -               1..2037
FT                   /note="pBR322 30..2066 2037bp
FT                   PvuII = CAG^CTG
FT                   BamHI =   G^GATCC"
FT   -               2038..2586
FT                   /note="SV40 1989..2537 549bp complement
FT                   PstI = CTGCA^G
FT                   HindIII =  A^AGCTT"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   promoter        0..0
FT                   /note="PRO SV40 late genes"
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SQ   Sequence 2586 BP; 538 A; 760 C; 673 G; 615 T; 0 other;
     agctttaatg cggtagttta tcacagttaa attgctaacg cagtcaggca ccgtgtatga
     aatctaacaa tgcgctcatc gtcatcctcg gcaccgtcac cctggatgct gtaggcatag
     gcttggttat gccggtactg ccgggcctct tgcgggatat cgtccattcc gacagcatcg
     ccagtcacta tggcgtgctg ctagcgctat atgcgttgat gcaatttcta tgcgcacccg
     ttctcggagc actgtccgac cgctttggcc gccgcccagt cctgctcgct tcgctacttg
     gagccactat cgactacgcg atcatggcga ccacacccgt cctgtggatc ctctacgccg
     gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg
     acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg
     tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac
     cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc
     aggagtcgca taagggagag cgtcgaccga tgcccttgag agccttcaac ccagtcagct
     ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca
     tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc
     gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg
     ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg
     ccggcatggc ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga
     tggccttccc cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg
     ccatgctgtc caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg
     ctcttaccag cctaacttcg atcactggac cgctgatcgt cacggcgatt tatgccgcct
     cggcgagcac atggaacggg ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc
     tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc gacctgaatg gaagccggcg
     gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac
     tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca tctccagcag
     ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct
     cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc
     accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac
     aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc
     ctgcaccatt atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc
     tacatctgta ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg
     catccatacc gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc
     agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag
     aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc
     ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga
     tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcaggat
     ccccaggaag ctcctctgtg tcctcataaa ccctaacctc ctctacttga gaggacattc
     caatcatagg ctgcccatcc accctctgtg tcctcctgtt aattaggtca cttaacaaaa
     aggaaattgg gtaggggttt ttcacagacc gctttctaag ggtaatttta aaatatctgg
     gaagtccctt ccactgctgt gttccagaag tgttggtaaa cagcccacaa atgtcaacag
     cagaaacata caagctgtca gctttgcaca agggcccaac accctgctca tcaagaagca
     ctgtggttgc tgtgttagta atgtgcaaaa caggaggcac attttcccca cctgtgtagg
     ttccaaaata tctagtgttt tcatttttac ttggatcagg aacccagcac tccactggat
     aagcattatc cttatccaaa acagccttgt ggtcagtgtt catctgctga ctgtcaactg
     tagcattttt tggggttaca gtttgagcag gatatttggt cctgtagttt gctaacacac
     cctgca
//