back Return to this vector's summary.
ID   PLG331     preliminary; circular DNA; SYN; 4635 BP.
XX
AC   IG9891;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pLG331 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pLG331 from pBR328
RC   pLG333 from pLG331 & pSC105
RC   pLG314 from pSC105
RC   pLG316 from pLG314
RC   pLG318 from pSC105 & pLG316
RC   pLG336 from pLG318
RC   pLG334 from pLG333
RC   pLG337 from pLG334 & pLG336
RC   pLG338 from pLG337
RC   pLG339 from pLG338 & pLG336
RA   Stoker N.G., Fairweather N.F., Spratt B.G.;
RT   "Versatile low-copy-number plasmid vectors for cloning in
RT   Escherichia coli";
RL   Gene 18:335-341(1982).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pLG331)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli C600)(E.coli)(E.coli K802)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR328)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR328 remove BamHI-SalI 276bp 376..652,
FT                   \ tet gene/4631bp
FT                   -> pLG331 4631bp [no SalI, no SmaI, unique HindIII]"
FT   -               1..4635
FT                   /note="pBR328 652..4907..379 4635bp
FT                   BamHI = G^GATC C
FT                   SalI =       G^TCGAC"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pSC101 (compatible with ColE1)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 4635 BP; 1090 A; 1218 C; 1172 G; 1155 T; 0 other;
     tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg
     cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc
     cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct
     gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
     tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
     tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
     cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
     ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
     atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc
     actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct
     tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc
     cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     ctataaaaat aggcgtatca cgaggccctt tcgtcttcga ataaatacct gtgacggaag
     atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
     acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
     aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
     ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
     tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
     ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
     acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
     ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
     tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
     ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
     acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag
     tgataataag cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca
     gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttaccggttt attgactacc
     ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc agtttgctca ggctctcccc
     gtggaggtaa taattgacga tatgatcatt tattctgcct cccagagcct gataaaaacg
     gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
     caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
     acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga
     tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
     ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
     gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
     gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacttct
     catgtttgac agcttatcat cgataagctt taatgcggta gtttatcaca gttaaattgc
     taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
     gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
     gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
     ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
     ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
     cccgtcctgt ggatc
//