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ID   PLGLACZ7   preliminary; circular DNA; SYN; 8787 BP.
XX
AC   D13251;
XX
DT   25-SEP-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Serratia marcescens plasmid vector pLGlacZ7 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-8787
RC   pLGlacZ7 from pBR322
RA   Omori K., Komatsubara S.;
RT   "Role of serine 352 in the allosteric response of Serratia
RT   marcescens aspartokinase I-homoserine dehydrogenase I analyzed by
RT   using site-directed mutagenesis";
RL   J. Bacteriol. 175:959-965(1992).
XX
RN   [2]
RC   pLGlacZ7
RA   Omori K.;
RT   ;
RL   Submitted (17-SEP-1992) to GenBank by:
RL   Omori K., Research Laboratory of Applied Biochemistry,
RL   Tanabe Seiyaku Co. Ltd., 16-89 Kashima-3-chome, Yodogawa-ku,
RL   Osaka 532, JAPAN.
XX
CC   NM (pLGlacZ7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (promoter analysis)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   -> pLGlacZ7 8787bp"
FT   misc_feature    0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 8787 BP; 2101 A; 2113 C; 2257 G; 2316 T; 0 other;
     ggatcccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc
     agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt
     cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg taacctatcc
     cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac
     atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt
     taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg
     tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt
     gatggtgctg cgttggagtg acggcagtta tctggaagat caggatatgt ggcggatgag
     cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca
     tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat
     gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca
     ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc
     cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc
     gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga
     agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg
     caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt
     catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc
     cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg
     cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct
     gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg
     cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc
     taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta
     tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt
     ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct
     acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct
     tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt
     cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca acccgtggtc
     ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct
     ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt
     tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca
     tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg
     tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc
     gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac
     cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct
     cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga
     tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc
     acagatgtgg attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg
     tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg
     ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac
     ggcagataca cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg
     gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat
     taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg
     ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta
     tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta
     taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta
     tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca
     actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat
     cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga
     attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata
     accgggcagg ggggatcaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta
     ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg
     cagaaattcg aaagcaaatt cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc
     gcttatgtct attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc
     ttctcaaatg cctgaggcca gtttgctcag gctctccccg tggaggtaat aattgacgat
     atgatcattt attctgcctc ccagagcctg ataaaaacgg ttagcgccgg ggttggattt
     ttcagcgttc cagctaaggc taaggcattc ctgttcaagc gcaagccggg ctataaagcg
     cattatcgct ttacccgtgc ccttatttcg atcgcactga gaaacataca gctctttaat
     atgcaggtaa tccgggcagc gcaacggaac attcatcagt gtaaaaatgg aatcaataaa
     gccctgcgca gcgcgcaggg tcagcctgaa tacgcgttta atgaccagca cagtcgtgat
     ggcaaggtca gaatagcgct gaggtctgcc tcgtgaagaa ggtgttgctg actcatacca
     ggcctgaatc gccccatcat ccagccagaa agtgagggag ccacggttga tgagagcttt
     gttgtaggtg gaccagttgg tgattttgaa cttttgcttt gccacggaac ggtctgcgtt
     gtcgggaaga tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag
     ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg
     gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
     agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct
     tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     tacgctgact tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag
     gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
     ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga
     cctcagcgct attctgacct tgccatcacg actgtgctgg tcattaaacg cgtattcagg
     ctgaccctgc gcgctgcgca gggctttatt gattccattt ttacactgat gaatgttccg
     ttgcgctgcc cggattacag ctgttcgggg gttcactgac gctgcgtgac tacgatggtc
     aggttgcgga ggctatggcc ctggtacgag cgctgaacaa aatgacgaaa gcaggtatgc
     ctgaaagcgt gcgtattgcc tgaaaacaca acccgctacg ggggagactt acccgaaatc
     tgatttattc aacaaagcca ctttcagtat ggatttgggt gttacggggg ggggttggtg
     cttaaaatcg cgctgaggat ttctgatggt gttaagcggg cggttttgag atgtaaactc
     gcccgtttaa cataatggat cttgcgcgca ccgcccgaac accactcgcc acaaaaaacc
     gccggaacgt ccaaaagtac gggttttgct gcccgcaaac gggctgttct ggtgttgcta
     gtttgttatc agaatcgcag atccggcttc aggtttgccg gctgaaagcg ctatttcttc
     cagaattgcc atgatttttt ccccacggga ggcgtcactg gctcccgtgt tgtcggcagc
     tttgattcga taagcagcat cgcctgtttc aggctgtcta tgtgtgactg ttgagctgta
     acaagttgtc tcaggtgttc aatttcatgt tctagttgct ttgttttact ggtttcacct
     gttctattag gtgttacatg ctgttcatct gttacattgt cgatctgttc atggtgaaca
     gctttgaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt tacaccgttt
     tcatctgtgc atatggacag ttttcccttt gatatgtaac ggtgaacagt tgttctactt
     ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac ctcagatcct
     tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa
     cgaaccattg agatcatact tactttgcat gtcactcaaa aattttgcct caaaactggt
     gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt tatgtaggta
     ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct ggttgttctc
     aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca acgtatcagt
     cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta aatctttact
     tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat tttcaagcat
     taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag ttttcttttg
     tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt caaaagactt
     aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag gcaatatctc
     ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg tccactggaa
     aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca tcagctctct
     ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca tctgagcgta
     ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa tcgtggggtt
     gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt catagcgact
     aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca attggtctag
     gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac tagtcctttt
     cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac ttgtaaattc
     tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt atattcaagt
     ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag atcccagccc
     tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt cgcaaacgct
     gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag
     ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg agtcgctgtc
     tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg ggtaaatggc
     actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa gaaaagcccg
     tcacgggctt ctcagggcgt tttatggcgg gtctgcggag tggtgaatcc gttagcgagg
     tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg
     ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg
     ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa
     gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca
     tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg
     ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc
     cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt
     gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc
     agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt
     gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga
     aggagctgac tgggttgaag gctctcaagg gcatcggtcg aattctgcag gtaccgcatg
     ctagctctag atctcacgtg gtcgact
//