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ID   PLTRPOLY   preliminary; circular DNA; SYN; 4273 BP.
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AC   X60280; ATCC77109;
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DT   23-OCT-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pLTRpoly - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-4273
RC   pLTRpoly from pGEM-7Zf+ & pUC19 & pSP72 & pSV2neo & pMP-1, MoMLV-LTR
RA   Maekelae T.P., Partanen J.M., Schwab M., Alitalo K.;
RT   "pLTRpoly: a versatile high efficiency mammalian expression vector";
RL   Gene 118:293-294(1992).
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RN   [2]
RP   1-4273
RC   pLTRpoly
RA   Maekelae T.P.;
RT   ;
RL   Submitted (01-SEP-1991) on tape to the EMBL Data Library by:
RL   T.P. Maekelae, Dept of Virology and Pathology, Univ of Helsinki,
RL   Haartmaninkatu 3, 00290 Helsinki, FINLAND.
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RN   [3]
RC   pMP-1 from MoMuLV LTR U3
RC   pMP-34.1K from pMP-1 & human N-myc gene
RC   pMP-34.1N from pMP-1 & human N-myc gene
RC   pNb-9K from human N-myc gene
RC   pNb-9N from human N-myc gene
RC   pNb-101 from human N-myc gene & lambda, cos
RA   Schwab M., Varmus H.E., Bishop J.M.;
RT   "Human N-myc gene contributes to neoplastic transformation of
RT   mammalian cells in culture";
RL   Nature 316:160-162(1985).
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RN   [4]
RC   pEJ(6.6) from c-Ha-ras-1 gene
RA   Shih C., Weinberg R.A.;
RT   "Isolation of a transforming sequence from a human bladder
RT   carcinoma cell line";
RL   Cell 29:161-169(1982).
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CC   Expression shuttle vector with promoters for in vitro RNA synthesis.
CC   (personal communication)
CC   Consists of the following sequences: nt 1-51 from pGEM7Zf(+) (reverse
CC   of 28-78), 52-70 from pUC19 (reverse of 428-446), 71-1090 from
CC   pSV2neo (reverse of 3050-4069), 1091-3474 from pSP72 (88-2462 and
CC   1-9), (personal communication)
CC   and 3475-4273 from pMP-1 (with 7505-8297 from MoMuLV). (personal
CC   communication)
CC   The order of the major features in this plasmid is:  HindIII site of
CC   MCS (nt = 1) - SV40 splice acceptor - SV40 late polyadenylation
CC   signal - T7 promoter - bla - SP6 promoter - XhoI site - MoMuLV LTR.
CC   (personal communication)
CC   Note that the T7 and SP6 promoters are not immediately adjacent to
CC   the cloning sites. (personal communication)
CC   When cotransfected with pSV2-neo (ATCC 37149) into NIH 3T3 cells,
CC   70-80% of G418-resistant colonies expressed high levels of
CC   appropriately sized mRNA. (personal communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--4.3; PstI--4.3; XhoI--3.6; 0.8. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pLTRpoly)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (mouse)(E.coli HB101)(E.coli DH5alpha)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)(expression up to 4000bp)
CC   SE ()
CC   PA (pGEM-7Zf+)(pUC19)(pSV2neo)(pSP72)(pMP-1)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. MoMuLV, LTR U3
FT                   -> pMP-1
FT                   1. pGEM-7Zf+ 50bp complement(28..78)
FT                   2. pUC19 HinfI-SphI 18bp 428..446,
FT                   \ MCS/complement(428..446)
FT                   3. pSV2neo 1019bp complement(3050..4069),
FT                   \ SV40 late polyA/acc
FT                   4. pSP72 2374bp 88..2462
FT                   5. pSP72 9bp 1..9
FT                   6. pMP-1 98bp 7505..7603, MoMLV-LTR
FT                   -> pLTRpoly 4273bp"
FT   misc_feature    1..107
FT                   /note="MCS HindIII-ClaI-AsuII-SmaI-KpnI-EcoRI-XhoI-
FT                   XbaI-SphI-PstI-SalI-AccI-HincII-NruI-SauI"
FT   misc_binding    0..0
FT                   /note="MCS unique HindIII-AsuII-SmaI-KpnI-EcoRI-
FT                   SphI-SalI-AccI-NruI-SauI"
FT   polyA_site      1039..1039
FT                   /note="PLA SV40 polyadenylation site"
FT   LTR             3475..4273
FT                   /note="RPT Moloney murine leukemia virus (Mo-MuLV)
FT                   3' long terminal repeat (LTR)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 4273 BP; 1154 A; 1005 C; 994 G; 1120 T; 0 other;
     aagcttatcg atttcgaacc cgggggtacc gaattcctcg agtctagagg agcatgcctg
     caggtcgacc gggctcgatc ccctcgcgag ttggttcagc tgctgcctga ggctggacga
     cctcgcggag ttctaccggc agtgcaaatc cgtcggcatc caggaaacca gcagcggcta
     tccgcgcatc catgcccccg aactgcagga gtggggaggc acgatggccg ctttggtccc
     ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     catgtctgga tctgccggtc tccctatagt gagtcgtatt aatttcgata agccaggtta
     acctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggacgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     agattgtact gagagtgcac catatggaca tattgtcgtt agaacgcggc tacaattaat
     acataacctt atgtatcata cacatacgat ttaggtgaca ctatagaact cgagcagagc
     ttccaaattg agagagaggc ttaatcagag acagaaactg tttgagtcaa ctcaaggatg
     gtttgaggga ctgtttaaca gatccccttg gtttaccacc ttgatatcta ccattatggg
     acccctcatt gtactcctaa tgattttgct cttcggaccc tgcattctta atcgattagt
     ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc aacaatatca
     ccagctgaag cctatagagt acgagccata gataaaataa aagattttat ttagtctcca
     gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg
     aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
     tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc ctccgattga
     ctgagtcgcc cgg
//