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ID   PMAC58     preliminary; circular DNA; SYN; 3803 BP.
XX
AC   L08930; A10238; A06419; A08598; VB0040;
XX
DT   04-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pMAC5-8 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3803
RC   pMAC5-8
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [2]
RC   pMAC5-bg14 from pMAC5-8
RC   pMAC5-8 from f1 or phage PF1 & pBR325 & pUC8 & trp promoter
RA   Stanssens P.;
RT   ;
RL   Unpublished (1991).
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RN   [3]
RC   pMAC5-8
RA   Van Rompuy L.;
RT   ;
RL   Submitted (00-XXX-1991) on magnetic tape by:
RL   van Rompuy L., Plant Gen. Systems, Gent, Belgium.
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Corrected 20-DEC-1986 by J. Thierack, MPI Biochemie, Martinsried
CC   Corrected 10-JUL-1987 by P. Stanssens and F. Pfeiffer
CC   Vector for multiple rounds of oligonucleotide-directed
CC   construction of  mutations. One derivative (pMA5) confers ampicillin
CC   resistance, the other (pMC5) confers resistance to chloramphenicol.
CC   Using specific oligonucleotides, resistance is shifted together with
CC   site-directed mutagenesis between these two resistance modes.
CC   The PstI site in the polylinker region is not unique.
CC   Presence of CAA at pos 2811-13 was shown by DNA sequencing. It
CC   is unknown, if this also applies to the parent sequence (pBR325)!
CC   NM (pMAC5-8)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(mutagenesis site-directed)
CC   SE ()
CC   PA (PF1)(pBR325)(Poly8)(EcoTrp)
CC   BR ()
CC   OF (pMAC5bg14)(pMAC7-8)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. phage PF1, ori
FT                   2. pBR325 TaqI-TaqI 773bp 4535..5308, cat gene
FT                   3. pUC8 2665bp
FT                   4. E. coli, trp promoter
FT                   -> pMAC5-8 3803bp"
FT   misc_feature    1..105
FT                   /note="from bacteriophage f1"
FT   misc_feature    111..215
FT                   /note="from bacteriophage f1"
FT   misc_feature    221..307
FT                   /note="from pBR325"
FT   rep_origin      313..768
FT                   /note="ORI bacteriophage f1"
FT   misc_feature    772..2575
FT                   /note="from pBR325"
FT   misc_feature    complement(2571..2684)
FT                   /note="from Tn903"
FT   misc_feature    complement(2718..2745)
FT                   /note="from EcoTrp"
FT   misc_feature    complement(2746..2773)
FT                   /note="from EcoTrp"
FT   misc_feature    complement(2773..3741)
FT                   /note="from pBR325"
FT   misc_binding    3754..3789
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-(PstI)-HindIII
FT                   M13mp8/pUC8"
FT   CDS             complement(1689..2477)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             complement(2861..3520)
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
XX
SQ   Sequence 3803 BP; 984 A; 930 C; 870 G; 1019 T; 0 other;
     aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt
     ttttttggag attttcaacg tgaaaaaatt attattcgca attccaagct aattcacctc
     gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt ttttttggag
     attttcaacg tgaaaaaatt attattcgca attccaagct ctgcctcgcg cgtttcggtg
     atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     cggatgcaga tcacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttga tctgcgctcg
     gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattatgt aagcagacag
     ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgactcccc gcgcgcgatg
     ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa aaagcccgct
     cattaggcgg gctcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
     aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
     catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg
     tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
     cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
     tttattcgaa gacgaaaggg catcgcgcgc ggggaattcc cggggatccg tcgacctgca
     gccaagcttg gtctagaggt cga
//