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ID   PMAC5BG14  preliminary; circular DNA; SYN; 7224 BP.
XX
AC   L08932; VB0041;
XX
DT   04-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMAC5-bg14 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7224
RC   pMAC5-bg14
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
RN   [2]
RC   pMAC5-bg14 from pMAC5-8
RC   pMAC5-8 from f1 or phage PF1 & pBR325 & pUC8 & trp promoter
RA   Stanssens P.;
RT   ;
RL   Unpublished (1991).
XX
RN   [3]
RC   pMAC5-bg14
RA   Van Rompuy L.;
RT   ;
RL   Submitted (00-XXX-1991) on magnetic tape by:
RL   van Rompuy L., Plant Gen. Systems, Gent, Belgium.
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Revised 3-FEB-1987 and 10-JUL-1987 by F. Pfeiffer according to
CC   the revised sequence of pMAC5-8.
CC   Vector for multiple rounds of oligonucleotide-directed
CC   construction of mutations. One derivative (pMA5) confers ampicillin
CC   resistance, the other (pMC5) confers resistance to chloramphenicol.
CC   Using specific oligonucleotides, resistance is shifted together with
CC   site-directed mutagenesis between these two resistance modes.
CC   The sites for EcoRI, BamHI, SalI and PstI in the polylinker
CC   region are not unique.
CC   NM (pMAC5-8)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(mutagenesis site-directed)
CC   SE ()
CC   PA (pMAC5-8)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMAC5-8 3803bp
FT                   2. E. coli, lacZ gene
FT                   -> pMAC5-bg14 7224bp"
FT   misc_feature    1..105
FT                   /note="from bacteriophage f1"
FT   misc_feature    111..215
FT                   /note="from bacteriophage f1"
FT   misc_feature    221..307
FT                   /note="from pBR325"
FT   rep_origin      313..768
FT                   /note="ORI bacteriophage f1"
FT   misc_feature    772..2575
FT                   /note="from pBR325"
FT   misc_feature    complement(2571..2684)
FT                   /note="from Tn903"
FT   misc_feature    complement(2718..2745)
FT                   /note="from EcoTrp"
FT   misc_feature    complement(2746..2773)
FT                   /note="from EcoTrp"
FT   misc_feature    complement(2773..3741)
FT                   /note="from pBR325"
FT   misc_feature    complement(3783..4029)
FT                   /note="from lambda"
FT   CDS             4111..7175
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    7175..7210
FT                   /note="MCS (EcoRI)-SmaI-(BamHI)-(SalI)-(PstI)-HindIII
FT                   M13mp8/pUC8"
FT   CDS             complement(1689..2477)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             complement(2860..3519)
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
XX
SQ   Sequence 7224 BP; 1778 A; 1848 C; 1838 G; 1760 T; 0 other;
     aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt
     ttttttggag attttcaacg tgaaaaaatt attattcgca attccaagct aattcacctc
     gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt ttttttggag
     attttcaacg tgaaaaaatt attattcgca attccaagct ctgcctcgcg cgtttcggtg
     atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     cggatgcaga tcacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttga tctgcgctcg
     gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattatgt aagcagacag
     ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga
     cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgactcccc gcgcgcgatg
     ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa aaagcccgct
     cattaggcgg gctcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
     aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact
     catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg
     tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
     cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
     tttattcgaa gacgaaaggg catcgcgcgc ggggaattgg ccacgatgcg tccggcgtag
     aggatctctc acctaccaaa caatgccccc ctgcaaaaaa taaattcata taaaaaacat
     acagataacc atctgcggtg ataaattatc tctggcggtg ttgacataaa taccactggc
     ggtgatactg agcacatcag caggacgcac tgaccaccat gaaggtgacg ctcttaaaat
     taagccctga agaagggcag cattcaaagc agaaggcttt ggggtgtgtg atacgaaacg
     aagcattgga attctacaac ttgcttggat tcctacaaag aagcagcaat tttcagtgtc
     agaagtcgac caaggaggtc tagatggatc ccgtcgtttt acaacgtcgt gactgggaaa
     accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc
     ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc
     ccatctacac caacgtaacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga
     atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc
     agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc aacgggcgct
     gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc gcatttttac
     gcgccggaga aaaccgcctc gcggtgatgg tgctgcgttg gagtgacggc agttatctgg
     aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg ctgcataaac
     cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat ttcagccgcg
     ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta cgggtaacag
     tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa
     ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac gtcgaaaacc
     cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg
     ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg
     aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt aaccgtcacg
     agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc
     tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac catccgctgt
     ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat attgaaaccc
     acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg gcgatgagcg
     aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc
     tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg
     tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata
     ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat
     ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat
     acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag gcgtttcgtc
     agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat
     atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat acgccgaacg
     atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga
     cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag
     tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg atggtggcgc
     tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca caaggtaaac
     agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc tggctcacag
     tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc agcgcctggc
     agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc cacgccatcc
     cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag cgttggcaat
     ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa caactgctga
     cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag
     cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg
     ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg ctgattacga
     ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa acctaccgga
     ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc gatacaccgc
     atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg gtaaactggc
     tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct
     gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc
     gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca
     acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat ctgctgcacg
     cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact
     cctggagccc gtcagtatcg gcggaattcc agctgagcgc cggtcgctac cattaccagt
     tggtctggtg tcaaaaataa taataaccgg gcaggaattc ccggggatcc gtcgacctgc
     agccaagctt ggtctagagg tcga
//