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ID   PMAKC76    preliminary; circular DNA; SYN; 1888 BP.
XX
AC   U08461;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pMAKC76 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-1814
RC   pMAKC76 from yeast 2 micron & T7 & Sp6 & R6K
RC   pMAKK76 from yeast 2 micron & T7 & Sp6 & R6K
RA   Posfai G., Koob M., Hradecna Z., Hasan N., Filutowicz M.,
RA   Szybalski W.;
RT   "In vivo excision and amplification of large fragments of the
RT   Escherichia coli genome";
RL   Nucleic Acids Res. 22:2392-2398(1994).
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RN   [2]
RP   1-1888
RC   pMAKC76
RA   Posfai G.;
RT   ;
RL   Submitted (07-APR-1994) by:
RL   Posfai G., University of Wisconsin, McArdle Lab. for Cancer Research,
RL   1400 University Avenue, Madison, WI 53706, USA.
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CC   NCBI gi: 475710
CC   NM (pMAKC76)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(mutagenesis site-directed)
CC   SE ()
CC   PA (yeast 2 micron)(T7)(Sp6)(R6K)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. yeast 2 micron, ori
FT                   2. T7, promoter
FT                   3. Sp6, promoter
FT                   4. R6K
FT                   -> pMAKC76 1888bp"
FT   misc_feature    17..50
FT                   /note="yeast 2 micron plasmid FRT site"
FT   misc_feature    103..103
FT                   /note="bacteriophage T7 RNA polymerase transcription
FT                   initiation site"
FT   misc_binding    112..168
FT                   /note="MCS"
FT   misc_feature    complement(174..174)
FT                   /note="bacteriophage SP6 RNA polymerase transcription
FT                   initiation site"
FT   rep_origin      430..805
FT                   /note="E. coli R6K gamma replication origin; LEFT"
FT   CDS             complement(930..1589)
FT                   /note="ANT E. coli chloramphenicol resistance gene"
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SQ   Sequence 1888 BP; 527 A; 399 C; 409 G; 553 T; 0 other;
     atcgatgaat tgatccgaag ttcctattct ctagaaagta taggaacttc gaattgtcga
     caagcttgat ctggcttatc gaaattaata cgactcacta tagggagacc ggaattcgag
     ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgt attctatagt
     gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
     ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
     acgccgttcc gcaccccgga aatggtcagc gaaccaatca gcagggtcat cgctagccca
     tggctaattc tgtcagccgt taagtgttcc tgtgtcactg aaaattgctt tgagaggctc
     taagggcttc tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta aaccttaaaa
     gctttaaaag ccttatatat tctttttttt cttataaaac ttaaaacctt agaggctatt
     taagttgctg atttatatta attttattgt tcaaacatga gagcttagta cgtgaaacat
     gagagcttag tacgttagcc atgagagctt agtacgttag ccatgagggt ttagttcgtt
     aaacatgaga gcttagtacg ttaaacatga gagcttagta cgtgaaacat gagagcttag
     tacgtactat caacaggttg aactgcggat cttgcggccg caaaaattaa aaatgaagtt
     ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     gtgaggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     tgttgtaatt cattaagcat tctgccgaca tggaagccat cacagacggc atgatgaacc
     tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaatttcgga
     tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg
     tgctccagtg gcttctgttt ctatcagc
//