ID   PMAKK76    preliminary; circular DNA; SYN; 1814 BP.
XX
AC   U08460;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMAKK76 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-1814
RC   pMAKC76 from yeast 2 micron & T7 & Sp6 & R6K
RC   pMAKK76 from yeast 2 micron & T7 & Sp6 & R6K
RA   Posfai G., Koob M., Hradecna Z., Hasan N., Filutowicz M.,
RA   Szybalski W.;
RT   "In vivo excision and amplification of large fragments of the
RT   Escherichia coli genome";
RL   Nucleic Acids Res. 22:2392-2398(1994).
XX
RN   [2]
RP   1-1814
RC   pMAKK76
RA   Posfai G.;
RT   ;
RL   Submitted (07-APR-1994) by:
RL   Posfai G., University of Wisconsin, McArdle Lab. for Cancer Research,
RL   1400 University Avenue, Madison, WI 53706, USA.
XX
CC   NCBI gi: 475708
CC   NM (pMAKK76)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(mutagenesis site-directed)
CC   SE ()
CC   PA (yeast 2 micron)(T7)(Sp6)(R6K)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. yeast 2 micron
FT                   2. T7, promoter
FT                   3. Sp6, promoter
FT                   4. R6K
FT                   -> pMAKK76 1814bp"
FT   misc_feature    17..50
FT                   /note="yeast 2 micron plasmid FRT site"
FT   misc_feature    103..103
FT                   /note="bacteriophage T7 RNA polymerase transcription
FT                   initiation site"
FT   misc_binding    112..168
FT                   /note="MCS"
FT   misc_feature    complement(174..174)
FT                   /note="bacteriophage SP6 RNA polymerase transcription
FT                   initiation site"
FT   rep_origin      430..805
FT                   /note="E. coli R6K gamma replication origin; LEFT"
FT   CDS             complement(869..1663)
FT                   /note="ANT E. coli kanamycin phosphotransferase gene
FT                   kanamycin resistance gene (kan)"
XX
SQ   Sequence 1814 BP; 463 A; 459 C; 444 G; 448 T; 0 other;
     atcgatgaat tgatccgaag ttcctattct ctagaaagta taggaacttc gaattgtcga
     caagcttgat ctggcttatc gaaattaata cgactcacta tagggagacc ggaattcgag
     ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgt attctatagt
     gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
     ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
     acgccgttcc gcaccccgga aatggtcagc gaaccaatca gcagggtcat cgctagccca
     tggctaattc tgtcagccgt taagtgttcc tgtgtcactg aaaattgctt tgagaggctc
     taagggcttc tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta aaccttaaaa
     gctttaaaag ccttatatat tctttttttt cttataaaac ttaaaacctt agaggctatt
     taagttgctg atttatatta attttattgt tcaaacatga gagcttagta cgtgaaacat
     gagagcttag tacgttagcc atgagagctt agtacgttag ccatgagggt ttagttcgtt
     aaacatgaga gcttagtacg ttaaacatga gagcttagta cgtgaaacat gagagcttag
     tacgtactat caacaggttg aactgcggat cttgcggccg caaaaattaa aaatgaagtt
     ttgacggtat cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atccgcgcct
     tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttggagttca ttcagggcac
     cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     ctgtctcttg atccactaga ttattgaagc atttatcagg gttattgtct catgagcgga
     tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     aaagtgccac ctgc
//