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ID PMALC2 preliminary; circular DNA; SYN; 6646 BP.
XX
AC IG1998; M24051; M24052; M21655; M21656;
XX
DT 01-JUN-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pMAL-c2 [tm] - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pPL-5A from malE gene
RC pCG806 from pUC18 & pmalE, malE gene
RC pCG150 from pUC18 & pPL-5A, malE gene
RC pCG325 from pCG150 & pMLB1034
RC pPL-6A' from pHI28 & pmalE & pCH2
RC pCG228 from pUC18 & pGW4400
RC pCG410 from pCG228 & pCG150
RC pCG828 from pCG228 & pCG806
RC pCG1001 from pACYC184 & modification methylase
RA Guan C.D., Li P., Riggs P.D., Inouye H.;
RT "Vectors that facilitate the expression and purification of
RT foreign peptides in Escherichia coli by fusion to maltose-binding
RT protein";
RL Gene 67:21-30(1988).
XX
CC NM (pMAL-c2 [tm])
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (NEB)
CC HO (E.coli)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (pKK233-2)
CC BR (pMAL-cRI [tm])
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. E. coli, malE gene
FT -> pMAL-c2 6646bp"
FT promoter 15..46
FT /note="PRO E. coli lacI gene"
FT misc_feature 81..83
FT /note="ATG E. coli lacI gene start codon (GTG)"
FT misc_feature 1161..1163
FT /note="TER E. coli lacI gene termination codon (TGA)"
FT misc_feature 1205..1205
FT /note="begin Ptac DNA fragment"
FT promoter 1406..1433
FT /note="PRO E. coli tac"
FT misc_feature 1478..1478
FT /note="begin E. coli malE DNA fragment"
FT misc_feature 1528..1528
FT /note="ATG E. coli malE start codon (ATG)"
FT misc_feature 2587..2610
FT /note="PRI E. coli malE primer #1237"
FT misc_feature 2629..2629
FT /note="MCS, also factor Xa site"
FT misc_feature 2735..2735
FT /note="start E. coli lacZ-alpha sequence (CA CTG GCC)"
FT misc_feature 2901..2901
FT /note="start E. coli rrnB terminator DNA fragment"
FT misc_feature 2914..2914
FT /note="TER first in-frame stop codon for E. coli
FT lacZ-alpha"
FT misc_feature 3327..3327
FT /note="start pBR322 sequence"
FT CDS 3418..3418
FT /note="E. coli beta-lactamase start codon (ATG)"
FT misc_feature 4276..4276
FT /note="E. coli beta-lactamase stop codon (TAA)"
FT rep_origin 4320..4833
FT /note="ORI bacteriophage f1 origin"
FT misc_feature 6646..6646
FT /note="end pBR322 sequence"
FT misc_binding 7..7
FT /note="SIT unique PflMI"
FT CDS 81..1161
FT /note="REP E. coli lacIq repressor gene"
FT misc_binding 431..431
FT /note="SIT unique MluI"
FT misc_binding 612..612
FT /note="SIT unique BstEII"
FT misc_binding 638..638
FT /note="SIT unique ApaI"
FT misc_binding 879..879
FT /note="SIT unique EcoRV"
FT misc_binding 935..935
FT /note="SIT unique HpaI"
FT misc_binding 1070..1070
FT /note="SIT unique NarI"
FT promoter 1406..1433
FT /note="PRO E. coli tac (trp -35 and lacUV5 -10)"
FT CDS 1528..2661
FT /note="GEN E. coli malE gene,
FT codes for maltose binding protein (MBP),
FT has no malE signal sequence"
FT misc_binding 1893..1893
FT /note="SIT unique BsiWI"
FT misc_binding 1962..1962
FT /note="SIT unique BglII"
FT misc_binding 2217..2217
FT /note="SIT unique BsmI"
FT misc_binding 2388..2388
FT /note="SIT unique Bpu1102I"
FT misc_binding 2564..2564
FT /note="SIT unique NcoI"
FT misc_binding 2704..2809
FT /note="MCS unique SacI-XmnI-EcoRI-BamHI-XbaI-SalI-
FT PstI-HindIII"
FT misc_binding 2763..2764
FT /note="SIT factor Xa"
FT CDS 2810..2968
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT terminator 2989..2989
FT /note="TER E. coli beta-galactosidase gene (lacZ)
FT stop codon"
FT terminator 0..0
FT /note="TER E. coli rrnB gene T1"
FT terminator 0..0
FT /note="TER E. coli rrnB gene T2"
FT CDS 3493..4351
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 3797..3797
FT /note="SIT unique ScaI"
FT misc_binding 4259..4259
FT /note="SIT unique Pfl108I"
FT misc_binding 4275..4275
FT /note="SIT unique Eam1105I"
FT rep_origin 4395..4908
FT /note="ORI bacteriophage M13 ori-, from pZ150"
FT misc_binding 4450..4450
FT /note="SIT unique SwaI"
FT misc_binding 4672..4672
FT /note="SIT unique DraIII"
FT misc_binding 4778..4778
FT /note="SIT unique NaeI"
FT rep_origin >4943..<5663
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 5607..5607
FT /note="ORI E. coli pMB1 (ColE1 and pBR322) start"
FT misc_binding 5785..5785
FT /note="SIT unique SapI"
FT misc_binding 5841..5841
FT /note="SIT unique NdeI"
FT misc_binding 5892..5892
FT /note="SIT unique AccI"
FT misc_binding 5916..5916
FT /note="SIT unique Tth111I"
FT CDS complement(>6000..<6378)
FT /note="REP E. coli ROP gene"
FT misc_binding 6137..6137
FT /note="SIT unique BspGI"
FT misc_binding 6557..6557
FT /note="SIT unique Bpu10I"
FT misc_binding 6694..6694
FT /note="SIT MscI"
XX
SQ Sequence 6646 BP; 1693 A; 1671 C; 1768 G; 1514 T; 0 other;
ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
gcacttcacc aacaaggacc atagattatg aaaatcgaag aaggtaaact ggtaatctgg
attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
ttggcgaaag atccacgtat tgccgccacc atggaaaacg cccagaaagg tgaaatcatg
ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat ttcagaattc
ggatcctcta gagtcgacct gcaggcaagc ttggcactgg ccgtcgtttt acaacgtcgt
gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
aatggcgaat ggcagcttgg ctgttttggc ggatgagata agattttcag cctgatacag
attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg
gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt
gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
cctttttgcg tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat
ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
gaacgttctc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
ccccggttga taatcagaaa agccccaaaa acaggaagat tgtataagca aatatttaaa
ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag cccgagatag
ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat
caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
ccgccgcgct taatgcgccg ctacagggcg cgtaaaagga tctaggtgaa gatccttttt
gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc
tgcggtaaag ctcatcagcg tggtcgtgca gcgattcaca gatgtctgcc tgttcatccg
cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca
tgttaagggc ggttttttcc tgtttggtca cttgatgcct ccgtgtaagg gggaatttct
gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt
tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga
ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag
cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gaaatt
//