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ID   PMALCRI    preliminary; circular DNA; SYN; 6133 BP.
XX
AC   IG1995; M24051; M24052; M21655; M21656;
XX
DT   01-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMAL-cRI [tm] - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pPL-5A from malE gene
RC   pCG806 from pUC18 & pmalE, malE gene
RC   pCG150 from pUC18 & pPL-5A, malE gene
RC   pCG325 from pCG150 & pMLB1034
RC   pPL-6A' from pHI28 & pmalE & pCH2
RC   pCG228 from pUC18 & pGW4400
RC   pCG410 from pCG228 & pCG150
RC   pCG828 from pCG228 & pCG806
RC   pCG1001 from pACYC184 & modification methylase
RA   Guan C.D., Li P., Riggs P.D., Inouye H.;
RT   "Vectors that facilitate the expression and purification of
RT   foreign peptides in Escherichia coli by fusion to maltose-binding
RT   protein";
RL   Gene 67:21-30(1988).
XX
CC   old NEB vector.
CC   NM (pMAL-cRI [tm])
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pKK233-2)
CC   BR (pMAL-c2 [tm])
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli, malE gene
FT                   -> pMAL-cRI 6133bp"
FT   promoter        15..46
FT                   /note="PRO E. coli lacI gene"
FT   misc_feature    81..83
FT                   /note="ATG E. coli lacI gene start codon (GTG)"
FT   misc_feature    1161..1163
FT                   /note="TER E. coli lacI gene termination codon (TGA)"
FT   misc_feature    1205..1205
FT                   /note="begin E. coli Ptac DNA fragment"
FT   promoter        1406..1433
FT                   /note="PRO E. coli tac"
FT   misc_feature    1478..1478
FT                   /note="begin E. coli malE DNA fragment"
FT   misc_feature    1528..1528
FT                   /note="ATG E. coli malE start codon (ATG)"
FT   misc_feature    2587..2610
FT                   /note="PRI E. coli malE primer #1237"
FT   misc_feature    2629..2629
FT                   /note="MCS, also factor Xa site"
FT   misc_feature    2717..2717
FT                   /note="start E. coli lacZ-alpha sequence (CA CTG GCC)"
FT   misc_feature    2883..2883
FT                   /note="start E. coli rrnB terminator DNA fragment"
FT   misc_feature    2896..2896
FT                   /note="TER first in-frame stop codon for E. coli
FT                   lacZ-alpha"
FT   misc_feature    3309..3309
FT                   /note="start pBR322 sequence"
FT   CDS             3400..3400
FT                   /note="E. coli beta-lactamase start codon (ATG)"
FT   misc_feature    4258..4258
FT                   /note="E. coli beta-lactamase stop codon (TAA)"
FT   misc_feature    6133..6133
FT                   /note="end pBR322 sequence"
FT   misc_binding    7..7
FT                   /note="SIT unique PflMI"
FT   CDS             81..1161
FT                   /note="REP E. coli lacIq repressor gene"
FT   misc_binding    431..431
FT                   /note="SIT unique MluI"
FT   misc_binding    612..612
FT                   /note="SIT unique BstEII"
FT   misc_binding    638..638
FT                   /note="SIT unique ApaI"
FT   misc_binding    879..879
FT                   /note="SIT unique EcoRV"
FT   misc_binding    935..935
FT                   /note="SIT unique HpaI"
FT   misc_binding    1070..1070
FT                   /note="SIT unique NarI"
FT   promoter        1406..1433
FT                   /note="PRO E. coli tac (trp -35 and lacUV5 -10)"
FT   CDS             1528..2661
FT                   /note="GEN E. coli malE gene,
FT                   codes for maltose binding protein (MBP),
FT                   has no malE signal sequence"
FT   misc_binding    1893..1893
FT                   /note="SIT unique BsiWI"
FT   misc_binding    1962..1962
FT                   /note="SIT unique BglII"
FT   misc_binding    2217..2217
FT                   /note="SIT unique BsmI"
FT   misc_binding    2388..2388
FT                   /note="SIT unique Bpu1102I"
FT   misc_binding    2564..2564
FT                   /note="SIT unique NcoI"
FT   misc_binding    2704..2809
FT                   /note="MCS unique SacI-XmnI-EcoRI-BamHI-XbaI-SalI-
FT                   PstI-HindIII"
FT   misc_binding    2763..2764
FT                   /note="SIT factor Xa"
FT   CDS             2810..2968
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   terminator      2989..2989
FT                   /note="TER E. coli beta-galactosidase gene (lacZ)
FT                   stop codon"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T1"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T2"
FT   CDS             3493..4351
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3797..3797
FT                   /note="SIT unique ScaI"
FT   misc_binding    4259..4259
FT                   /note="SIT unique Pfl108I"
FT   misc_binding    4275..4275
FT                   /note="SIT unique Eam1105I"
FT   rep_origin      4395..4908
FT                   /note="ORI bacteriophage M13 ori-, from pZ150"
FT   misc_binding    4450..4450
FT                   /note="SIT unique SwaI"
FT   misc_binding    4672..4672
FT                   /note="SIT unique DraIII"
FT   misc_binding    4778..4778
FT                   /note="SIT unique NaeI"
FT   rep_origin      >4943..<5663
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      5607..5607
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322) start"
FT   misc_binding    5785..5785
FT                   /note="SIT unique SapI"
FT   misc_binding    5841..5841
FT                   /note="SIT unique NdeI"
FT   misc_binding    5892..5892
FT                   /note="SIT unique AccI"
FT   misc_binding    5916..5916
FT                   /note="SIT unique Tth111I"
FT   CDS             complement(>6000..<6378)
FT                   /note="REP E. coli ROP gene"
FT   misc_binding    6137..6137
FT                   /note="SIT unique BspGI"
FT   misc_binding    6557..6557
FT                   /note="SIT unique Bpu10I"
FT   misc_binding    6694..6694
FT                   /note="SIT MscI"
XX
SQ   Sequence 6133 BP; 1518 A; 1556 C; 1640 G; 1419 T; 0 other;
     ccgacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
     gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
     gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
     cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
     aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
     acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
     tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
     ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
     ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
     cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
     tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
     cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
     cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
     atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
     tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
     acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
     gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     gcacttcacc aacaaggacc atagattatg aaaatcgaag aaggtaaact ggtaatctgg
     attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     ccgaacaaag agctggcgaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     ttggcgaaag atccacgtat tgccgccacc atggaaaacg cccagaaagg tgaaatcatg
     ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     gtaccgtcct ctctcgtgat cgagggaagg atttcagaat tcggatcctc tagagtcgac
     ctgcaggcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcagctt
     ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga
     agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc
     atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg
     agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc
     ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac
     tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca
     aactcttttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg
     attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta
     atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactt
     gatgcctccg tgtaaggggg aatttctgtt catgggggta atgataccga tgaaacgaga
     gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga
     gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg
     ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat
     gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac
     acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc
     gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac
     gctgcccgaa att
//