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ID PMALP preliminary; circular DNA; SYN; 6223 BP.
XX
AC IG1996; M24051; M24052; M21655; M21656;
XX
DT 01-JUN-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pMAL-p [tm] - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pPL-5A from malE gene
RC pCG806 from pUC18 & pmalE, malE gene
RC pCG150 from pUC18 & pPL-5A, malE gene
RC pCG325 from pCG150 & pMLB1034
RC pPL-6A' from pHI28 & pmalE & pCH2
RC pCG228 from pUC18 & pGW4400
RC pCG410 from pCG228 & pCG150
RC pCG828 from pCG228 & pCG806
RC pCG1001 from pACYC184 & modification methylase
RA Guan C.D., Li P., Riggs P.D., Inouye H.;
RT "Vectors that facilitate the expression and purification of
RT foreign peptides in Escherichia coli by fusion to maltose-binding
RT protein";
RL Gene 67:21-30(1988).
XX
CC old NEB vector.
CC NM (pMAL-p [tm])
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (pKK233-2)
CC BR (pMAL-c2 [tm])
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. E. coli, malE gene
FT -> pMAL-p 6223bp"
FT promoter 15..46
FT /note="PRO E. coli lacI gene"
FT misc_feature 81..83
FT /note="ATG E. coli lacI gene start codon (GTG)"
FT misc_feature 1161..1163
FT /note="TER E. coli lacI gene termination codon (TGA)"
FT misc_feature 1205..1205
FT /note="begin Ptac DNA fragment"
FT promoter 1406..1433
FT /note="PRO E. coli tac"
FT misc_feature 1478..1478
FT /note="begin E. coli malE DNA fragment"
FT misc_feature 1528..1528
FT /note="ATG E. coli malE start codon (ATG)"
FT misc_feature 2662..2685
FT /note="PRI E. coli malE primer #1237"
FT misc_feature 2704..2704
FT /note="MCS, also factor Xa site"
FT misc_feature 2804..2804
FT /note="start E. coli lacZ-alpha sequence (CA CTG GCC)"
FT misc_feature 2970..2970
FT /note="start E. coli rrnB terminator DNA fragment"
FT misc_feature 2983..2983
FT /note="TER first in-frame stop codon for E. coli
FT lacZ-alpha"
FT misc_feature 3396..3396
FT /note="start pBR322 sequence"
FT CDS 3487..3489
FT /note="E. coli beta-lactamase start codon (ATG)"
FT misc_feature 4345..4347
FT /note="E. coli beta-lactamase stop codon (TAA)"
FT misc_feature 6220..6220
FT /note="end pBR322 sequence"
FT misc_binding 7..7
FT /note="SIT unique PflMI"
FT CDS 81..1161
FT /note="REP E. coli lacIq repressor gene"
FT misc_binding 431..431
FT /note="SIT unique MluI"
FT misc_binding 612..612
FT /note="SIT unique BstEII"
FT misc_binding 638..638
FT /note="SIT unique ApaI"
FT misc_binding 879..879
FT /note="SIT unique EcoRV"
FT misc_binding 935..935
FT /note="SIT unique HpaI"
FT misc_binding 1070..1070
FT /note="SIT unique NarI"
FT promoter 1406..1433
FT /note="PRO E. coli tac (trp -35 and lacUV5 -10)"
FT CDS 1528..2661
FT /note="GEN E. coli malE gene,
FT codes for maltose binding protein (MBP),
FT has malE signal sequence"
FT misc_feature 1531..1605
FT /note="GEN E. coli malE gene signal sequence"
FT misc_binding 1893..1893
FT /note="SIT unique BsiWI"
FT misc_binding 1962..1962
FT /note="SIT unique BglII"
FT misc_binding 2217..2217
FT /note="SIT unique BsmI"
FT misc_binding 2388..2388
FT /note="SIT unique Bpu1102I"
FT misc_binding 2564..2564
FT /note="SIT unique NcoI"
FT misc_binding 2704..2809
FT /note="MCS unique SacI-XmnI-EcoRI-BamHI-XbaI-SalI-
FT PstI-HindIII"
FT misc_binding 2763..2764
FT /note="SIT factor Xa"
FT CDS 2810..2968
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT terminator 2989..2989
FT /note="TER E. coli beta-galactosidase gene (lacZ)
FT stop codon"
FT terminator 0..0
FT /note="TER E. coli rrnB gene T1"
FT terminator 0..0
FT /note="TER E. coli rrnB gene T2"
FT CDS 3493..4351
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 3797..3797
FT /note="SIT unique ScaI"
FT misc_binding 4259..4259
FT /note="SIT unique Pfl108I"
FT misc_binding 4275..4275
FT /note="SIT unique Eam1105I"
FT rep_origin 4395..4908
FT /note="ORI bacteriophage M13 ori-, from pZ150"
FT misc_binding 4450..4450
FT /note="SIT unique SwaI"
FT misc_binding 4672..4672
FT /note="SIT unique DraIII"
FT misc_binding 4778..4778
FT /note="SIT unique NaeI"
FT rep_origin >4943..<5663
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 5607..5607
FT /note="ORI E. coli pMB1 (ColE1 and pBR322) start"
FT misc_binding 5785..5785
FT /note="SIT unique SapI"
FT misc_binding 5841..5841
FT /note="SIT unique NdeI"
FT misc_binding 5892..5892
FT /note="SIT unique AccI"
FT misc_binding 5916..5916
FT /note="SIT unique Tth111I"
FT CDS complement(>6000..<6378)
FT /note="REP E. coli ROP gene"
FT misc_binding 6137..6137
FT /note="SIT unique BspGI"
FT misc_binding 6557..6557
FT /note="SIT unique Bpu10I"
FT misc_binding 6694..6694
FT /note="SIT MscI"
XX
SQ Sequence 6223 BP; 1542 A; 1586 C; 1659 G; 1436 T; 0 other;
ccgacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
gcacttcacc aacaaggacc atagattatg aaaataaaaa caggtgcacg catcctcgca
ttatccgcat taacgacgat gatgttttcc gcctcggctc tcgccaaaat cgaagaaggt
aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag
aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag
aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac
cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc
caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct
tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg
ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc
gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg
ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac
gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat
gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc
atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg
gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt
attaacgccg ccagtccgaa caaagagctg gcgaaagagt tcctcgaaaa ctatctgctg
actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag
tcttacgagg aagagttggc gaaagatcca cgtattgccg ccaccatgga aaacgcccag
aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact
gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag
actaattcga gctcggtacc cggccgggga tccatcgagg gtaggcctga attcagtaaa
cctaccctcg atggatcctc tagagtcgac ctgcaggcaa gcttggcact ggccgtcgtt
ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
ttgcgcagcc tgaatggcga atggcagctt ggctgttttg gcggatgaga gaagattttc
agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
cctgacggat ggcctttttg cgtttctaca aactcttttt gtttattttt ctaaatacat
tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
taagggcggt tttttcctgt ttggtcactt gatgcctccg tgtaaggggg aatttctgtt
catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
gatcatgcgc acccgtggcc aggacccaac gctgcccgaa att
//