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ID   PMALP      preliminary; circular DNA; SYN; 6223 BP.
XX
AC   IG1996; M24051; M24052; M21655; M21656;
XX
DT   01-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMAL-p [tm] - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pPL-5A from malE gene
RC   pCG806 from pUC18 & pmalE, malE gene
RC   pCG150 from pUC18 & pPL-5A, malE gene
RC   pCG325 from pCG150 & pMLB1034
RC   pPL-6A' from pHI28 & pmalE & pCH2
RC   pCG228 from pUC18 & pGW4400
RC   pCG410 from pCG228 & pCG150
RC   pCG828 from pCG228 & pCG806
RC   pCG1001 from pACYC184 & modification methylase
RA   Guan C.D., Li P., Riggs P.D., Inouye H.;
RT   "Vectors that facilitate the expression and purification of
RT   foreign peptides in Escherichia coli by fusion to maltose-binding
RT   protein";
RL   Gene 67:21-30(1988).
XX
CC   old NEB vector.
CC   NM (pMAL-p [tm])
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pKK233-2)
CC   BR (pMAL-c2 [tm])
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli, malE gene
FT                   -> pMAL-p 6223bp"
FT   promoter        15..46
FT                   /note="PRO E. coli lacI gene"
FT   misc_feature    81..83
FT                   /note="ATG E. coli lacI gene start codon (GTG)"
FT   misc_feature    1161..1163
FT                   /note="TER E. coli lacI gene termination codon (TGA)"
FT   misc_feature    1205..1205
FT                   /note="begin Ptac DNA fragment"
FT   promoter        1406..1433
FT                   /note="PRO E. coli tac"
FT   misc_feature    1478..1478
FT                   /note="begin E. coli malE DNA fragment"
FT   misc_feature    1528..1528
FT                   /note="ATG E. coli malE start codon (ATG)"
FT   misc_feature    2662..2685
FT                   /note="PRI E. coli malE primer #1237"
FT   misc_feature    2704..2704
FT                   /note="MCS, also factor Xa site"
FT   misc_feature    2804..2804
FT                   /note="start E. coli lacZ-alpha sequence (CA CTG GCC)"
FT   misc_feature    2970..2970
FT                   /note="start E. coli rrnB terminator DNA fragment"
FT   misc_feature    2983..2983
FT                   /note="TER first in-frame stop codon for E. coli
FT                   lacZ-alpha"
FT   misc_feature    3396..3396
FT                   /note="start pBR322 sequence"
FT   CDS             3487..3489
FT                   /note="E. coli beta-lactamase start codon (ATG)"
FT   misc_feature    4345..4347
FT                   /note="E. coli beta-lactamase stop codon (TAA)"
FT   misc_feature    6220..6220
FT                   /note="end pBR322 sequence"
FT   misc_binding    7..7
FT                   /note="SIT unique PflMI"
FT   CDS             81..1161
FT                   /note="REP E. coli lacIq repressor gene"
FT   misc_binding    431..431
FT                   /note="SIT unique MluI"
FT   misc_binding    612..612
FT                   /note="SIT unique BstEII"
FT   misc_binding    638..638
FT                   /note="SIT unique ApaI"
FT   misc_binding    879..879
FT                   /note="SIT unique EcoRV"
FT   misc_binding    935..935
FT                   /note="SIT unique HpaI"
FT   misc_binding    1070..1070
FT                   /note="SIT unique NarI"
FT   promoter        1406..1433
FT                   /note="PRO E. coli tac (trp -35 and lacUV5 -10)"
FT   CDS             1528..2661
FT                   /note="GEN E. coli malE gene,
FT                   codes for maltose binding protein (MBP),
FT                   has malE signal sequence"
FT   misc_feature    1531..1605
FT                   /note="GEN E. coli malE gene signal sequence"
FT   misc_binding    1893..1893
FT                   /note="SIT unique BsiWI"
FT   misc_binding    1962..1962
FT                   /note="SIT unique BglII"
FT   misc_binding    2217..2217
FT                   /note="SIT unique BsmI"
FT   misc_binding    2388..2388
FT                   /note="SIT unique Bpu1102I"
FT   misc_binding    2564..2564
FT                   /note="SIT unique NcoI"
FT   misc_binding    2704..2809
FT                   /note="MCS unique SacI-XmnI-EcoRI-BamHI-XbaI-SalI-
FT                   PstI-HindIII"
FT   misc_binding    2763..2764
FT                   /note="SIT factor Xa"
FT   CDS             2810..2968
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   terminator      2989..2989
FT                   /note="TER E. coli beta-galactosidase gene (lacZ)
FT                   stop codon"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T1"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T2"
FT   CDS             3493..4351
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3797..3797
FT                   /note="SIT unique ScaI"
FT   misc_binding    4259..4259
FT                   /note="SIT unique Pfl108I"
FT   misc_binding    4275..4275
FT                   /note="SIT unique Eam1105I"
FT   rep_origin      4395..4908
FT                   /note="ORI bacteriophage M13 ori-, from pZ150"
FT   misc_binding    4450..4450
FT                   /note="SIT unique SwaI"
FT   misc_binding    4672..4672
FT                   /note="SIT unique DraIII"
FT   misc_binding    4778..4778
FT                   /note="SIT unique NaeI"
FT   rep_origin      >4943..<5663
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      5607..5607
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322) start"
FT   misc_binding    5785..5785
FT                   /note="SIT unique SapI"
FT   misc_binding    5841..5841
FT                   /note="SIT unique NdeI"
FT   misc_binding    5892..5892
FT                   /note="SIT unique AccI"
FT   misc_binding    5916..5916
FT                   /note="SIT unique Tth111I"
FT   CDS             complement(>6000..<6378)
FT                   /note="REP E. coli ROP gene"
FT   misc_binding    6137..6137
FT                   /note="SIT unique BspGI"
FT   misc_binding    6557..6557
FT                   /note="SIT unique Bpu10I"
FT   misc_binding    6694..6694
FT                   /note="SIT MscI"
XX
SQ   Sequence 6223 BP; 1542 A; 1586 C; 1659 G; 1436 T; 0 other;
     ccgacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
     gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
     gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
     cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
     aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
     acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
     tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
     ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
     ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
     cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
     tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
     cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
     cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
     atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
     tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
     acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
     gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     gcacttcacc aacaaggacc atagattatg aaaataaaaa caggtgcacg catcctcgca
     ttatccgcat taacgacgat gatgttttcc gcctcggctc tcgccaaaat cgaagaaggt
     aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag
     aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag
     aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac
     cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc
     caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct
     tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg
     ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc
     gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg
     ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac
     gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat
     gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc
     atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg
     gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt
     attaacgccg ccagtccgaa caaagagctg gcgaaagagt tcctcgaaaa ctatctgctg
     actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag
     tcttacgagg aagagttggc gaaagatcca cgtattgccg ccaccatgga aaacgcccag
     aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact
     gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag
     actaattcga gctcggtacc cggccgggga tccatcgagg gtaggcctga attcagtaaa
     cctaccctcg atggatcctc tagagtcgac ctgcaggcaa gcttggcact ggccgtcgtt
     ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     ttgcgcagcc tgaatggcga atggcagctt ggctgttttg gcggatgaga gaagattttc
     agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc
     ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc
     gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa
     acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg
     agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat
     cctgacggat ggcctttttg cgtttctaca aactcttttt gtttattttt ctaaatacat
     tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     taagggcggt tttttcctgt ttggtcactt gatgcctccg tgtaaggggg aatttctgtt
     catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     gatcatgcgc acccgtggcc aggacccaac gctgcccgaa att
//