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ID PMALP2 preliminary; circular DNA; SYN; 6721 BP.
XX
AC IG1999; M24051; M24052; M21655; M21656;
XX
DT 01-JUN-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pMAL-p2 [tm] - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pPL-5A from malE gene
RC pCG806 from pUC18 & pmalE, malE gene
RC pCG150 from pUC18 & pPL-5A, malE gene
RC pCG325 from pCG150 & pMLB1034
RC pPL-6A' from pHI28 & pmalE & pCH2
RC pCG228 from pUC18 & pGW4400
RC pCG410 from pCG228 & pCG150
RC pCG828 from pCG228 & pCG806
RC pCG1001 from pACYC184 & modification methylase
RA Guan C.D., Li P., Riggs P.D., Inouye H.;
RT "Vectors that facilitate the expression and purification of
RT foreign peptides in Escherichia coli by fusion to maltose-binding
RT protein";
RL Gene 67:21-30(1988).
XX
CC NM (pMAL-p2 [tm])
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (NEB)
CC HO (E.coli)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (pKK233-2)
CC BR (pMAL-c2 [tm])
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. E. coli, malE gene
FT -> pMAL-p2 6721bp"
FT promoter 15..46
FT /note="PRO E. coli lacI gene"
FT misc_feature 81..83
FT /note="ATG E. coli lacI gene start codon (GTG)"
FT misc_feature 1161..1163
FT /note="TER E. coli lacI gene termination codon (TGA)"
FT misc_feature 1205..1205
FT /note="begin E. coli Ptac DNA fragment"
FT promoter 1406..1433
FT /note="PRO E. coli tac"
FT misc_feature 1478..1478
FT /note="begin E. coli malE DNA fragment"
FT misc_feature 1528..1528
FT /note="ATG E. coli malE start codon (ATG)"
FT misc_feature 2662..2685
FT /note="PRI E. coli malE primer #1237"
FT misc_feature 2704..2704
FT /note="MCS, also factor Xa site"
FT misc_feature 2810..2810
FT /note="start E. coli lacZ-alpha sequence (CA CTG GCC)"
FT misc_feature 2976..2976
FT /note="start E. coli rrnB terminator DNA fragment"
FT misc_feature 2989..2989
FT /note="TER first in-frame stop codon for E. coli
FT lacZ-alpha"
FT misc_feature 3402..3402
FT /note="start pBR322 sequence"
FT CDS 3493..3493
FT /note="E. coli beta-lactamase start codon (ATG)"
FT misc_feature 4351..4351
FT /note="E. coli beta-lactamase stop codon (TAA)"
FT rep_origin 4395..4908
FT /note="ORI bacteriophage M13 origin (+ -)"
FT misc_feature 6721..6721
FT /note="end pBR322 sequence"
FT misc_binding 7..7
FT /note="SIT unique PflMI"
FT CDS 81..1161
FT /note="REP E. coli lacIq repressor gene"
FT misc_binding 431..431
FT /note="SIT unique MluI"
FT misc_binding 612..612
FT /note="SIT unique BstEII"
FT misc_binding 638..638
FT /note="SIT unique ApaI"
FT misc_binding 879..879
FT /note="SIT unique EcoRV"
FT misc_binding 935..935
FT /note="SIT unique HpaI"
FT misc_binding 1070..1070
FT /note="SIT unique NarI"
FT promoter 1406..1433
FT /note="PRO E. coli tac (trp -35 and lacUV5 -10)"
FT CDS 1528..2661
FT /note="GEN E. coli malE gene,
FT codes for maltose binding protein (MBP),
FT has malE signal sequence"
FT misc_feature 1531..1605
FT /note="GEN E. coli malE gene signal sequence"
FT misc_binding 1893..1893
FT /note="SIT unique BsiWI"
FT misc_binding 1962..1962
FT /note="SIT unique BglII"
FT misc_binding 2217..2217
FT /note="SIT unique BsmI"
FT misc_binding 2388..2388
FT /note="SIT unique Bpu1102I"
FT misc_binding 2564..2564
FT /note="SIT unique NcoI"
FT misc_binding 2704..2809
FT /note="MCS unique SacI-XmnI-EcoRI-BamHI-XbaI-SalI-
FT PstI-HindIII"
FT misc_binding 2763..2764
FT /note="SIT factor Xa"
FT CDS 2810..2968
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT terminator 2989..2989
FT /note="TER E. coli beta-galactosidase gene (lacZ)
FT stop codon"
FT terminator 0..0
FT /note="TER E. coli rrnB gene T1"
FT terminator 0..0
FT /note="TER E. coli rrnB gene T2"
FT CDS 3493..4351
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 3797..3797
FT /note="SIT unique ScaI"
FT misc_binding 4259..4259
FT /note="SIT unique Pfl108I"
FT misc_binding 4275..4275
FT /note="SIT unique Eam1105I"
FT rep_origin 4395..4908
FT /note="ORI bacteriophage M13 ori-, from pZ150"
FT misc_binding 4450..4450
FT /note="SIT unique SwaI"
FT misc_binding 4672..4672
FT /note="SIT unique DraIII"
FT misc_binding 4778..4778
FT /note="SIT unique NaeI"
FT rep_origin >4943..<5663
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 5607..5607
FT /note="ORI E. coli pMB1 (ColE1 and pBR322) start"
FT misc_binding 5785..5785
FT /note="SIT unique SapI"
FT misc_binding 5841..5841
FT /note="SIT unique NdeI"
FT misc_binding 5892..5892
FT /note="SIT unique AccI"
FT misc_binding 5916..5916
FT /note="SIT unique Tth111I"
FT CDS complement(>6000..<6378)
FT /note="REP E. coli ROP gene"
FT misc_binding 6137..6137
FT /note="SIT unique BspGI"
FT misc_binding 6557..6557
FT /note="SIT unique Bpu10I"
FT misc_binding 6694..6694
FT /note="SIT MscI"
XX
SQ Sequence 6721 BP; 1713 A; 1694 C; 1782 G; 1532 T; 0 other;
ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
gcacttcacc aacaaggacc atagattatg aaaataaaaa caggtgcacg catcctcgca
ttatccgcat taacgacgat gatgttttcc gcctcggctc tcgccaaaat cgaagaaggt
aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag
aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag
aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac
cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc
caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct
tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg
ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc
gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg
ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac
gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat
gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc
atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg
gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt
attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg
actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag
tcttacgagg aagagttggc gaaagatcca cgtattgccg ccaccatgga aaacgcccag
aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact
gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag
actaattcga gctcgaacaa caacaacaat aacaataaca acaacctcgg gatcgaggga
aggatttcag aattcggatc ctctagagtc gacctgcagg caagcttggc actggccgtc
gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
cagttgcgca gcctgaatgg cgaatggcag cttggctgtt ttggcggatg agataagatt
ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc
catcctgacg gatggccttt ttgcgtttct acaaactctt tttgtttatt tttctaaata
cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct gctatgtggc
gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg aagattgtat
aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt
aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtaa aaggatctag
gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgcagcgat tcacagatgt
ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcacttga tgcctccgtg
taagggggaa tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac
gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact
ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt
taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa
cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa
gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgaaat
t
//