back Return to this vector's summary.
ID   PMALP2     preliminary; circular DNA; SYN; 6721 BP.
XX
AC   IG1999; M24051; M24052; M21655; M21656;
XX
DT   01-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMAL-p2 [tm] - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pPL-5A from malE gene
RC   pCG806 from pUC18 & pmalE, malE gene
RC   pCG150 from pUC18 & pPL-5A, malE gene
RC   pCG325 from pCG150 & pMLB1034
RC   pPL-6A' from pHI28 & pmalE & pCH2
RC   pCG228 from pUC18 & pGW4400
RC   pCG410 from pCG228 & pCG150
RC   pCG828 from pCG228 & pCG806
RC   pCG1001 from pACYC184 & modification methylase
RA   Guan C.D., Li P., Riggs P.D., Inouye H.;
RT   "Vectors that facilitate the expression and purification of
RT   foreign peptides in Escherichia coli by fusion to maltose-binding
RT   protein";
RL   Gene 67:21-30(1988).
XX
CC   NM (pMAL-p2 [tm])
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (NEB)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pKK233-2)
CC   BR (pMAL-c2 [tm])
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli, malE gene
FT                   -> pMAL-p2 6721bp"
FT   promoter        15..46
FT                   /note="PRO E. coli lacI gene"
FT   misc_feature    81..83
FT                   /note="ATG E. coli lacI gene start codon (GTG)"
FT   misc_feature    1161..1163
FT                   /note="TER E. coli lacI gene termination codon (TGA)"
FT   misc_feature    1205..1205
FT                   /note="begin E. coli Ptac DNA fragment"
FT   promoter        1406..1433
FT                   /note="PRO E. coli tac"
FT   misc_feature    1478..1478
FT                   /note="begin E. coli malE DNA fragment"
FT   misc_feature    1528..1528
FT                   /note="ATG E. coli malE start codon (ATG)"
FT   misc_feature    2662..2685
FT                   /note="PRI E. coli malE primer #1237"
FT   misc_feature    2704..2704
FT                   /note="MCS, also factor Xa site"
FT   misc_feature    2810..2810
FT                   /note="start E. coli lacZ-alpha sequence (CA CTG GCC)"
FT   misc_feature    2976..2976
FT                   /note="start E. coli rrnB terminator DNA fragment"
FT   misc_feature    2989..2989
FT                   /note="TER first in-frame stop codon for E. coli
FT                   lacZ-alpha"
FT   misc_feature    3402..3402
FT                   /note="start pBR322 sequence"
FT   CDS             3493..3493
FT                   /note="E. coli beta-lactamase start codon (ATG)"
FT   misc_feature    4351..4351
FT                   /note="E. coli beta-lactamase stop codon (TAA)"
FT   rep_origin      4395..4908
FT                   /note="ORI bacteriophage M13 origin (+ -)"
FT   misc_feature    6721..6721
FT                   /note="end pBR322 sequence"
FT   misc_binding    7..7
FT                   /note="SIT unique PflMI"
FT   CDS             81..1161
FT                   /note="REP E. coli lacIq repressor gene"
FT   misc_binding    431..431
FT                   /note="SIT unique MluI"
FT   misc_binding    612..612
FT                   /note="SIT unique BstEII"
FT   misc_binding    638..638
FT                   /note="SIT unique ApaI"
FT   misc_binding    879..879
FT                   /note="SIT unique EcoRV"
FT   misc_binding    935..935
FT                   /note="SIT unique HpaI"
FT   misc_binding    1070..1070
FT                   /note="SIT unique NarI"
FT   promoter        1406..1433
FT                   /note="PRO E. coli tac (trp -35 and lacUV5 -10)"
FT   CDS             1528..2661
FT                   /note="GEN E. coli malE gene,
FT                   codes for maltose binding protein (MBP),
FT                   has malE signal sequence"
FT   misc_feature    1531..1605
FT                   /note="GEN E. coli malE gene signal sequence"
FT   misc_binding    1893..1893
FT                   /note="SIT unique BsiWI"
FT   misc_binding    1962..1962
FT                   /note="SIT unique BglII"
FT   misc_binding    2217..2217
FT                   /note="SIT unique BsmI"
FT   misc_binding    2388..2388
FT                   /note="SIT unique Bpu1102I"
FT   misc_binding    2564..2564
FT                   /note="SIT unique NcoI"
FT   misc_binding    2704..2809
FT                   /note="MCS unique SacI-XmnI-EcoRI-BamHI-XbaI-SalI-
FT                   PstI-HindIII"
FT   misc_binding    2763..2764
FT                   /note="SIT factor Xa"
FT   CDS             2810..2968
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   terminator      2989..2989
FT                   /note="TER E. coli beta-galactosidase gene (lacZ)
FT                   stop codon"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T1"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB gene T2"
FT   CDS             3493..4351
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3797..3797
FT                   /note="SIT unique ScaI"
FT   misc_binding    4259..4259
FT                   /note="SIT unique Pfl108I"
FT   misc_binding    4275..4275
FT                   /note="SIT unique Eam1105I"
FT   rep_origin      4395..4908
FT                   /note="ORI bacteriophage M13 ori-, from pZ150"
FT   misc_binding    4450..4450
FT                   /note="SIT unique SwaI"
FT   misc_binding    4672..4672
FT                   /note="SIT unique DraIII"
FT   misc_binding    4778..4778
FT                   /note="SIT unique NaeI"
FT   rep_origin      >4943..<5663
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      5607..5607
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322) start"
FT   misc_binding    5785..5785
FT                   /note="SIT unique SapI"
FT   misc_binding    5841..5841
FT                   /note="SIT unique NdeI"
FT   misc_binding    5892..5892
FT                   /note="SIT unique AccI"
FT   misc_binding    5916..5916
FT                   /note="SIT unique Tth111I"
FT   CDS             complement(>6000..<6378)
FT                   /note="REP E. coli ROP gene"
FT   misc_binding    6137..6137
FT                   /note="SIT unique BspGI"
FT   misc_binding    6557..6557
FT                   /note="SIT unique Bpu10I"
FT   misc_binding    6694..6694
FT                   /note="SIT MscI"
XX
SQ   Sequence 6721 BP; 1713 A; 1694 C; 1782 G; 1532 T; 0 other;
     ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
     gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
     gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
     cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
     aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
     acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
     tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
     ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
     ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
     cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
     tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
     cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
     cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
     atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
     tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
     acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
     gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     gcacttcacc aacaaggacc atagattatg aaaataaaaa caggtgcacg catcctcgca
     ttatccgcat taacgacgat gatgttttcc gcctcggctc tcgccaaaat cgaagaaggt
     aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag
     aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag
     aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac
     cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc
     caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct
     tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg
     ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc
     gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg
     ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac
     gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat
     gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc
     atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg
     gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt
     attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg
     actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag
     tcttacgagg aagagttggc gaaagatcca cgtattgccg ccaccatgga aaacgcccag
     aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact
     gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag
     actaattcga gctcgaacaa caacaacaat aacaataaca acaacctcgg gatcgaggga
     aggatttcag aattcggatc ctctagagtc gacctgcagg caagcttggc actggccgtc
     gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     cagttgcgca gcctgaatgg cgaatggcag cttggctgtt ttggcggatg agataagatt
     ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct
     ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt
     agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat
     aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga agcaacggcc
     cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta agcagaaggc
     catcctgacg gatggccttt ttgcgtttct acaaactctt tttgtttatt tttctaaata
     cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct gctatgtggc
     gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg aagattgtat
     aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt
     aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtaa aaggatctag
     gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgcagcgat tcacagatgt
     ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
     tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcacttga tgcctccgtg
     taagggggaa tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac
     gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact
     ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt
     taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa
     cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa
     gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
     ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
     cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgaaat
     t
//