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ID   PMAM       preliminary; circular DNA; SYN; 7632 BP.
XX
AC   IG1084; M81768; J03163;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMAM - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pMAM from lambda]
RC   [pMAMneo from pMAM & neo gene]
RC   from pMAMneo
RC   from EMBL3
RA   Sardet C., Franchi A., Pouyssegur J.;
RT   "Molecular cloning, primary structure, and expression of the human
RT   growth factor-activatable Na+/H+ antiporter";
RL   Cell 56:271-280(1989).
XX
CC   NM (pMAM)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(COS cells)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF (pMAMneo)(pMAMneo-CAT)(pMAMneo-LUC)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. MMTV, LTR
FT                   3. SV40, early splice/polyA/promoter
FT                   4. E. coli, gpt gene
FT                   -> pMAM 7632bp"
FT   misc_binding    1..1
FT                   /note="SIT HindIII"
FT   promoter        1..1453
FT                   /note="PRO mouse mammary tumor virus (MMTV)
FT                   long terminal repeat (LTR)"
FT   misc_binding    326..326
FT                   /note="SIT ClaI"
FT   misc_binding    1078..1078
FT                   /note="SIT HindIII"
FT   misc_binding    1453..1472
FT                   /note="MCS NheI-SmaI-SalI-XhoI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   misc_binding    2190..2190
FT                   /note="SIT HpaI"
FT   misc_binding    2323..2323
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   misc_binding    2598..2598
FT                   /note="SIT HindIII"
FT   CDS             2598..0
FT                   /note="GEN E. coli gpt gene"
FT   misc_binding    2718..2718
FT                   /note="SIT BglII"
FT   misc_binding    2916..2916
FT                   /note="SIT KpnI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   misc_binding    4369..4369
FT                   /note="SIT HpaI"
FT   misc_binding    4502..4502
FT                   /note="SIT BamHI"
FT   misc_binding    5047..5047
FT                   /note="SIT PstI"
FT   misc_binding    5262..5262
FT                   /note="SIT EcoRI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    5889..5889
FT                   /note="SIT PvuI"
FT   misc_binding    6015..6015
FT                   /note="SIT PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    7402..7402
FT                   /note="SIT Tth111I"
XX
SQ   Sequence 7632 BP; 2078 A; 1736 C; 1703 G; 2115 T; 0 other;
     aagcttgggc agaaatggtt gaactcccga gagtgtccta cacctagggg agaagcagcc
     aaggggttgt ttcccaccaa ggacgacccg tctgcgcaca aacggatgag cccatcagac
     aaagacatat tcattctctg ctgcaaactt ggcatagctc tgctttgcct ggggctattg
     ggggaagttg cggttcgtgc tcgcagggct ctcacccttg actcttttaa tagctcttct
     gtgcaagatt acaatctaaa caattcggag aactcgacct tcctcctgag gcaaggacca
     cagccaactt cctcttacaa gccgcatcga ttttgtcctt cagaaataga aataagaatg
     cttgctaaaa attatatttt taccaataag accaatccaa taggtagatt attagttact
     atgttaagaa atgaatcatt atcttttagt actattttta ctcaaattca gaagttagaa
     atgggaatag aaaatagaaa gagacgctca acctcaattg aagaacaggt gcaaggacta
     ttgaccacag gcctagaagt aaaaaaggga aaaaagagtg tttttgtcaa aataggagac
     aggtggtggc aaccagggac ttatagggga ccttacatct acagaccaac agatgccccc
     ttaccatata caggaagata tgacttaaat tgggataggt gggttacagt caatggctat
     aaagtgttat atagatccct cccttttcgt gaaagactcg ccagagctag acctccttgg
     tgtatgttgt ctcaagaaga aaaagacgac atgaaacaac aggtacatga ttatatttat
     ctaggaacag gaatgcactt ttggggaaag attttccata ccaaggaggg gacagtggct
     ggactaatag aacattattc tgcaaaaact catggcatga gttattatga atagccttta
     ttggcccaac cttgcggttc ccagggctta agtaagtttt tggttacaaa ctgttcttaa
     aacgaggatg tgagacaagt ggtttcctga cttggtttgg tatcaaaggt tctgatctga
     gctctgagtg ttctattttc ctatgttctt ttggaattta tccaaatctt atgtaaatgc
     ttatgtaaac caagatataa aagagtgctg attttttgag taaacttgca acagtcctaa
     cattcacctc ttgtgtgttt gtgtctgttc gccatcccgt ctccgctcgt cacttatcct
     tcactttcca gagggtcccc ccgcagaccc cggcgaccct caggtcggcc gactgcggca
     gctggcgccc gaacagggac cctcggataa gtgacccttg tctctatttc tactatttgg
     tgtttgtctt gtattgtctc tttcttgtct ggctatcatc acaagagcgg aacggactca
     ccatagggac caagctagcc cggggtcgac cggtcgacct cgagggatct ttgtgaagga
     accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
     acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
     tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
     taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccggc
     tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta
     ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
     ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
     actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
     atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
     ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     atcaatgtat cttatcatgt ctggatcccc aggaagctcc tctgtgtcct cataaaccct
     aacctcctct acttgagagg acattccaat cataggctgc ccatccaccc tctgtgtcct
     cctgttaatt aggtcactta acaaaaagga aattgggtag gggtttttca cagaccgctt
     tctaagggta attttaaaat atctgggaag tcccttccac tgctgtgttc cagaagtgtt
     ggtaaacagc ccacaaatgt caacagcaga aacatacaag ctgtcagctt tgcacaaggg
     cccaacaccc tgctcatcaa gaagcactgt ggttgctgtg ttagtaatgt gcaaaacagg
     aggcacattt tccccacctg tgtaggttcc aaaatatcta gtgttttcat ttttacttgg
     atcaggaacc cagcactcca ctggataagc attatcctta tccaaaacag ccttgtggtc
     agtgttcatc tgctgactgt caactgtagc attttttggg gttacagttt gagcaggata
     tttggtcctg tagtttgcta acacaccctg cagctccaaa ggttccccac caacagcaaa
     aaaatgaaaa tttgaccctt gaatgggttt tccagcacca ttttcatgag ttttttgtgt
     ccctgaatgc aagtttaaca tagcagttac cccaataacc tcagttttaa cagtaacagc
     ttcccacatc aaaatatttc cacaggttaa gtcctcattt aaattaggca aaggaattct
     tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     gcgcgaggca gc
//