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ID PMAM preliminary; circular DNA; SYN; 7632 BP.
XX
AC IG1084; M81768; J03163;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pMAM - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC [pMAM from lambda]
RC [pMAMneo from pMAM & neo gene]
RC from pMAMneo
RC from EMBL3
RA Sardet C., Franchi A., Pouyssegur J.;
RT "Molecular cloning, primary structure, and expression of the human
RT growth factor-activatable Na+/H+ antiporter";
RL Cell 56:271-280(1989).
XX
CC NM (pMAM)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (CLONTECH)
CC HO (E.coli)(COS cells)
CC CP ()
CC FN (expression)
CC SE ()
CC PA (pBR322)
CC BR ()
CC OF (pMAMneo)(pMAMneo-CAT)(pMAMneo-LUC)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pBR322 4361bp, ori/amp gene
FT 2. MMTV, LTR
FT 3. SV40, early splice/polyA/promoter
FT 4. E. coli, gpt gene
FT -> pMAM 7632bp"
FT misc_binding 1..1
FT /note="SIT HindIII"
FT promoter 1..1453
FT /note="PRO mouse mammary tumor virus (MMTV)
FT long terminal repeat (LTR)"
FT misc_binding 326..326
FT /note="SIT ClaI"
FT misc_binding 1078..1078
FT /note="SIT HindIII"
FT misc_binding 1453..1472
FT /note="MCS NheI-SmaI-SalI-XhoI"
FT misc_feature 0..0
FT /note="SPL SV40 early genes"
FT polyA_signal 0..0
FT /note="PLA SV40 early genes"
FT misc_binding 2190..2190
FT /note="SIT HpaI"
FT misc_binding 2323..2323
FT /note="SIT BamHI"
FT rep_origin 0..0
FT /note="ORI SV40"
FT promoter 0..0
FT /note="PRO SV40 early genes"
FT misc_binding 2598..2598
FT /note="SIT HindIII"
FT CDS 2598..0
FT /note="GEN E. coli gpt gene"
FT misc_binding 2718..2718
FT /note="SIT BglII"
FT misc_binding 2916..2916
FT /note="SIT KpnI"
FT misc_feature 0..0
FT /note="SPL SV40 early genes"
FT polyA_signal 0..0
FT /note="PLA SV40 early genes"
FT misc_binding 4369..4369
FT /note="SIT HpaI"
FT misc_binding 4502..4502
FT /note="SIT BamHI"
FT misc_binding 5047..5047
FT /note="SIT PstI"
FT misc_binding 5262..5262
FT /note="SIT EcoRI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 5889..5889
FT /note="SIT PvuI"
FT misc_binding 6015..6015
FT /note="SIT PstI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 7402..7402
FT /note="SIT Tth111I"
XX
SQ Sequence 7632 BP; 2078 A; 1736 C; 1703 G; 2115 T; 0 other;
aagcttgggc agaaatggtt gaactcccga gagtgtccta cacctagggg agaagcagcc
aaggggttgt ttcccaccaa ggacgacccg tctgcgcaca aacggatgag cccatcagac
aaagacatat tcattctctg ctgcaaactt ggcatagctc tgctttgcct ggggctattg
ggggaagttg cggttcgtgc tcgcagggct ctcacccttg actcttttaa tagctcttct
gtgcaagatt acaatctaaa caattcggag aactcgacct tcctcctgag gcaaggacca
cagccaactt cctcttacaa gccgcatcga ttttgtcctt cagaaataga aataagaatg
cttgctaaaa attatatttt taccaataag accaatccaa taggtagatt attagttact
atgttaagaa atgaatcatt atcttttagt actattttta ctcaaattca gaagttagaa
atgggaatag aaaatagaaa gagacgctca acctcaattg aagaacaggt gcaaggacta
ttgaccacag gcctagaagt aaaaaaggga aaaaagagtg tttttgtcaa aataggagac
aggtggtggc aaccagggac ttatagggga ccttacatct acagaccaac agatgccccc
ttaccatata caggaagata tgacttaaat tgggataggt gggttacagt caatggctat
aaagtgttat atagatccct cccttttcgt gaaagactcg ccagagctag acctccttgg
tgtatgttgt ctcaagaaga aaaagacgac atgaaacaac aggtacatga ttatatttat
ctaggaacag gaatgcactt ttggggaaag attttccata ccaaggaggg gacagtggct
ggactaatag aacattattc tgcaaaaact catggcatga gttattatga atagccttta
ttggcccaac cttgcggttc ccagggctta agtaagtttt tggttacaaa ctgttcttaa
aacgaggatg tgagacaagt ggtttcctga cttggtttgg tatcaaaggt tctgatctga
gctctgagtg ttctattttc ctatgttctt ttggaattta tccaaatctt atgtaaatgc
ttatgtaaac caagatataa aagagtgctg attttttgag taaacttgca acagtcctaa
cattcacctc ttgtgtgttt gtgtctgttc gccatcccgt ctccgctcgt cacttatcct
tcactttcca gagggtcccc ccgcagaccc cggcgaccct caggtcggcc gactgcggca
gctggcgccc gaacagggac cctcggataa gtgacccttg tctctatttc tactatttgg
tgtttgtctt gtattgtctc tttcttgtct ggctatcatc acaagagcgg aacggactca
ccatagggac caagctagcc cggggtcgac cggtcgacct cgagggatct ttgtgaagga
accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccggc
tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctggta
ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
atcaatgtat cttatcatgt ctggatcccc aggaagctcc tctgtgtcct cataaaccct
aacctcctct acttgagagg acattccaat cataggctgc ccatccaccc tctgtgtcct
cctgttaatt aggtcactta acaaaaagga aattgggtag gggtttttca cagaccgctt
tctaagggta attttaaaat atctgggaag tcccttccac tgctgtgttc cagaagtgtt
ggtaaacagc ccacaaatgt caacagcaga aacatacaag ctgtcagctt tgcacaaggg
cccaacaccc tgctcatcaa gaagcactgt ggttgctgtg ttagtaatgt gcaaaacagg
aggcacattt tccccacctg tgtaggttcc aaaatatcta gtgttttcat ttttacttgg
atcaggaacc cagcactcca ctggataagc attatcctta tccaaaacag ccttgtggtc
agtgttcatc tgctgactgt caactgtagc attttttggg gttacagttt gagcaggata
tttggtcctg tagtttgcta acacaccctg cagctccaaa ggttccccac caacagcaaa
aaaatgaaaa tttgaccctt gaatgggttt tccagcacca ttttcatgag ttttttgtgt
ccctgaatgc aagtttaaca tagcagttac cccaataacc tcagttttaa cagtaacagc
ttcccacatc aaaatatttc cacaggttaa gtcctcattt aaattaggca aaggaattct
tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
gcgcgaggca gc
//