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ID   PMAMNEO    preliminary; circular DNA; SYN; 8413 BP.
XX
AC   IG1085; M81768; J03163;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMAMneo - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pMAM from lambda]
RC   [pMAMneo from pMAM & neo gene]
RC   from pMAMneo
RC   from EMBL3
RA   Sardet C., Franchi A., Pouyssegur J.;
RT   "Molecular cloning, primary structure, and expression of the human
RT   growth factor-activatable Na+/H+ antiporter";
RL   Cell 56:271-280(1989).
XX
CC   NM (pMAMneo)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(COS cells)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pMAM)
CC   BR ()
CC   OF (pMAMneo-CAT)(pMAMneo-LUC)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMAM 7632bp
FT                   2. Tn5 PvuII-PvuII 762bp 1786..2548, neo gene
FT                   -> pMAMneo 8413bp"
FT   misc_binding    1..1
FT                   /note="SIT EcoRI"
FT   promoter        1..91
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   misc_binding    91..91
FT                   /note="SIT HindIII"
FT   promoter        91..1543
FT                   /note="PRO mouse mammary tumor virus (MMTV)
FT                   long terminal repeat (LTR)"
FT   misc_binding    417..417
FT                   /note="SIT ClaI"
FT   misc_binding    1172..1172
FT                   /note="SIT SacI"
FT   misc_binding    1453..1472
FT                   /note="MCS NheI-SmaI-SalI-XhoI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   misc_binding    2282..2282
FT                   /note="SIT HpaI"
FT   misc_binding    2413..2413
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   misc_binding    2688..2688
FT                   /note="SIT HindIII"
FT   CDS             2598..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo)"
FT   misc_binding    3008..3008
FT                   /note="SIT BglII"
FT   misc_binding    3225..3225
FT                   /note="SIT PstI"
FT   misc_binding    4011..4011
FT                   /note="SIT SmaI"
FT   misc_binding    4148..4148
FT                   /note="SIT PstI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   misc_binding    4898..4898
FT                   /note="SIT HpaI"
FT   misc_binding    5029..5029
FT                   /note="SIT BamHI"
FT   misc_binding    5578..5578
FT                   /note="SIT PstI"
FT   misc_binding    5780..5780
FT                   /note="SIT EcoRI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    6409..6409
FT                   /note="SIT PvuI"
FT   misc_binding    6660..6660
FT                   /note="SIT BglI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    7845..7845
FT                   /note="SIT NdeI"
FT   misc_binding    8278..8278
FT                   /note="SIT PvuI"
XX
SQ   Sequence 8413 BP; 2228 A; 1922 C; 2002 G; 2261 T; 0 other;
     gaattccgca ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgccatt
     tgaccattca ccacattggt gtgcacctcc aagcttgggc agaaatggtt gaactcccga
     gagtgtccta cacctagggg agaagcagcc aaggggttgt ttcccaccaa ggacgacccg
     tctgcgcaca aacggatgag cccatcagac aaagacatat tcattctctg ctgcaaactt
     ggcatagctc tgctttgcct ggggctattg ggggaagttg cggttcgtgc tcgcagggct
     ctcacccttg actcttttaa tagctcttct gtgcaagatt acaatctaaa caattcggag
     aactcgacct tcctcctgag gcaaggacca cagccaactt cctcttacaa gccgcatcga
     ttttgtcctt cagaaataga aataagaatg cttgctaaaa attatatttt taccaataag
     accaatccaa taggtagatt attagttact atgttaagaa atgaatcatt atcttttagt
     actattttta ctcaaattca gaagttagaa atgggaatag aaaatagaaa gagacgctca
     acctcaattg aagaacaggt gcaaggacta ttgaccacag gcctagaagt aaaaaaggga
     aaaaagagtg tttttgtcaa aataggagac aggtggtggc aaccagggac ttatagggga
     ccttacatct acagaccaac agatgccccc ttaccatata caggaagata tgacttaaat
     tgggataggt gggttacagt caatggctat aaagtgttat atagatccct cccttttcgt
     gaaagactcg ccagagctag acctccttgg tgtatgttgt ctcaagaaga aaaagacgac
     atgaaacaac aggtacatga ttatatttat ctaggaacag gaatgcactt ttggggaaag
     attttccata ccaaggaggg gacagtggct ggactaatag aacattattc tgcaaaaact
     catggcatga gttattatga atagccttta ttggcccaac cttgcggttc ccagggctta
     agtaagtttt tggttacaaa ctgttcttaa aacgaggatg tgagacaagt ggtttcctga
     cttggtttgg tatcaaaggt tctgatctga gctctgagtg ttctattttc ctatgttctt
     ttggaattta tccaaatctt atgtaaatgc ttatgtaaac caagatataa aagagtgctg
     attttttgag taaacttgca acagtcctaa cattcacctc ttgtgtgttt gtgtctgttc
     gccatcccgt ctccgctcgt cacttatcct tcactttcca gagggtcccc ccgcagaccc
     cggcgaccct caggtcggcc gactgcggca gctggcgccc gaacagggac cctcggataa
     gtgacccttg tctctatttc tactatttgg tgtttgtctt gtattgtctc tttcttgtct
     ggctatcatc acaagagcgg aacggactca ccatagggac caagctagcc cggggtcgac
     cggtcgacct cgagggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     tcaatgtatc ttatcatgtc tggatccggc tgtggaatgt gtgtcagtta gggtgtggaa
     agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     tttgcaaaaa gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg
     gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
     ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
     tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
     tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
     gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat gaggatcgtt
     tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     ttcgcccacc ccgggctcga tcccctcgcg agttggttca gctgctgcct gaggctggac
     gacctcgcgg agttctaccg gcagtgcaaa tccgtcggca tccaggaaac cagcagcggc
     tatccgcgca tccatgcccc cgaactgcag gagtggggag gcacgatggc cgctttggtc
     ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     atcatgtctg gatccccagg aagctcctct gtgtcctcat aaaccctaac ctcctctact
     tgagaggaca ttccaatcat aggctgccca tccaccctct gtgtcctcct gttaattagg
     tcacttaaca aaaaggaaat tgggtagggg tttttcacag accgctttct aagggtaatt
     ttaaaatatc tgggaagtcc cttccactgc tgtgttccag aagtgttggt aaacagccca
     caaatgtcaa cagcagaaac atacaagctg tcagctttgc acaagggccc aacaccctgc
     tcatcaagaa gcactgtggt tgctgtgtta gtaatgtgca aaacaggagg cacattttcc
     ccacctgtgt aggttccaaa atatctagtg ttttcatttt tacttggatc aggaacccag
     cactccactg gataagcatt atccttatcc aaaacagcct tgtggtcagt gttcatctgc
     tgactgtcaa ctgtagcatt ttttggggtt acagtttgag caggatattt ggtcctgtag
     tttgctaaca caccctgcag ctccaaaggt tccccaccaa cagcaaaaaa atgaaaattt
     gacccttgaa tgggttttcc agcaccattt tcatgagttt tttgtgtccc tgaatgcaag
     tttaacatag cagttacccc aataacctca gttttaacag taacagcttc ccacatcaaa
     atatttccac aggttaagtc ctcatttaaa ttaggcaaag gaattcttga agacgaaagg
     gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     tgcttcgcga tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc
     gaaaagcggg gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct
     tttgcatagg gagggggaaa tgtagtctta tgcaatacac ttgtagtctt gcaacatggt
     aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     tggacgaacc act
//