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ID   PMAMNEOCAT preliminary; circular DNA; SYN; 9184 BP.
XX
AC   IG1086;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMAMneo-CAT - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [pMAM from lambda]
RC   [pMAMneo from pMAM & neo gene]
RC   from pMAMneo
RC   from EMBL3
RA   Sardet C., Franchi A., Pouyssegur J.;
RT   "Molecular cloning, primary structure, and expression of the human
RT   growth factor-activatable Na+/H+ antiporter";
RL   Cell 56:271-280(1989).
XX
CC   NM (pMAMneo-CAT)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(COS cells)
CC   CP ()
CC   FN (expression)(reporter)
CC   SE ()
CC   PA (pMAMneo)
CC   BR (pMAMneo-LUC)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMAMneo 8413bp
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pMAMneo-CAT 9184bp"
FT   misc_binding    1..1
FT                   /note="SIT EcoRI"
FT   promoter        1..91
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   misc_binding    91..91
FT                   /note="SIT HindIII"
FT   promoter        91..1543
FT                   /note="PRO mouse mammary tumor virus (MMTV)
FT                   long terminal repeat (LTR)"
FT   misc_binding    417..417
FT                   /note="SIT ClaI"
FT   misc_binding    1172..1172
FT                   /note="SIT SacI"
FT   misc_binding    6933..6933
FT                   /note="MCS NheI-SmaI-SalI-CAT gene-SalI-XhoI"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_feature    0..0
FT                   /note="SPL SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPTII), neomycin resistance gene (neo)"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   misc_binding    0..0
FT                   /note="SIT BglI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT NdeI"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
XX
SQ   Sequence 9184 BP; 2439 A; 2083 C; 2181 G; 2481 T; 0 other;
     gaattccgca ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgccatt
     tgaccattca ccacattggt gtgcacctcc aagcttgggc agaaatggtt gaactcccga
     gagtgtccta cacctagggg agaagcagcc aaggggttgt ttcccaccaa ggacgacccg
     tctgcgcaca aacggatgag cccatcagac aaagacatat tcattctctg ctgcaaactt
     ggcatagctc tgctttgcct ggggctattg ggggaagttg cggttcgtgc tcgcagggct
     ctcacccttg actcttttaa tagctcttct gtgcaagatt acaatctaaa caattcggag
     aactcgacct tcctcctgag gcaaggacca cagccaactt cctcttacaa gccgcatcga
     ttttgtcctt cagaaataga aataagaatg cttgctaaaa attatatttt taccaataag
     accaatccaa taggtagatt attagttact atgttaagaa atgaatcatt atcttttagt
     actattttta ctcaaattca gaagttagaa atgggaatag aaaatagaaa gagacgctca
     acctcaattg aagaacaggt gcaaggacta ttgaccacag gcctagaagt aaaaaaggga
     aaaaagagtg tttttgtcaa aataggagac aggtggtggc aaccagggac ttatagggga
     ccttacatct acagaccaac agatgccccc ttaccatata caggaagata tgacttaaat
     tgggataggt gggttacagt caatggctat aaagtgttat atagatccct cccttttcgt
     gaaagactcg ccagagctag acctccttgg tgtatgttgt ctcaagaaga aaaagacgac
     atgaaacaac aggtacatga ttatatttat ctaggaacag gaatgcactt ttggggaaag
     attttccata ccaaggaggg gacagtggct ggactaatag aacattattc tgcaaaaact
     catggcatga gttattatga atagccttta ttggcccaac cttgcggttc ccagggctta
     agtaagtttt tggttacaaa ctgttcttaa aacgaggatg tgagacaagt ggtttcctga
     cttggtttgg tatcaaaggt tctgatctga gctctgagtg ttctattttc ctatgttctt
     ttggaattta tccaaatctt atgtaaatgc ttatgtaaac caagatataa aagagtgctg
     attttttgag taaacttgca acagtcctaa cattcacctc ttgtgtgttt gtgtctgttc
     gccatcccgt ctccgctcgt cacttatcct tcactttcca gagggtcccc ccgcagaccc
     cggcgaccct caggtcggcc gactgcggca gctggcgccc gaacagggac cctcggataa
     gtgacccttg tctctatttc tactatttgg tgtttgtctt gtattgtctc tttcttgtct
     ggctatcatc acaagagcgg aacggactca ccatagggac caagctagcc cggggtcgac
     gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
     tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
     gcctggtgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgtcgacc
     tcgagggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     cttatcatgt ctggatccgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     agcttcacgc tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta
     gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat
     tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     cccgggctcg atcccctcgc gagttggttc agctgctgcc tgaggctgga cgacctcgcg
     gagttctacc ggcagtgcaa atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc
     atccatgccc ccgaactgca ggagtgggga ggcacgatgg ccgctttggt cccggatctt
     tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
     tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
     ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
     tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     ggatccccag gaagctcctc tgtgtcctca taaaccctaa cctcctctac ttgagaggac
     attccaatca taggctgccc atccaccctc tgtgtcctcc tgttaattag gtcacttaac
     aaaaaggaaa ttgggtaggg gtttttcaca gaccgctttc taagggtaat tttaaaatat
     ctgggaagtc ccttccactg ctgtgttcca gaagtgttgg taaacagccc acaaatgtca
     acagcagaaa catacaagct gtcagctttg cacaagggcc caacaccctg ctcatcaaga
     agcactgtgg ttgctgtgtt agtaatgtgc aaaacaggag gcacattttc cccacctgtg
     taggttccaa aatatctagt gttttcattt ttacttggat caggaaccca gcactccact
     ggataagcat tatccttatc caaaacagcc ttgtggtcag tgttcatctg ctgactgtca
     actgtagcat tttttggggt tacagtttga gcaggatatt tggtcctgta gtttgctaac
     acaccctgca gctccaaagg ttccccacca acagcaaaaa aatgaaaatt tgacccttga
     atgggttttc cagcaccatt ttcatgagtt ttttgtgtcc ctgaatgcaa gtttaacata
     gcagttaccc caataacctc agttttaaca gtaacagctt cccacatcaa aatatttcca
     caggttaagt cctcatttaa attaggcaaa ggaattcttg aagacgaaag ggcctcgtga
     tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     atgtacgggc cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg
     ggcttcggtt gtacgcggtt aggagtcccc tcaggatata gtagtttcgc ttttgcatag
     ggagggggaa atgtagtctt atgcaataca cttgtagtct tgcaacatgg taacgatgag
     ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     cact
//