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ID   PMAMNEOLUC preliminary; circular DNA; SYN; 10339 BP.
XX
AC   IG1087;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMAMneo-LUC - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSCLUC from pSC11 & pDO432
RC   WRLUC from pSCLUC & vaccinia virus
RA   Rodriguez J.F., Rodriguez D., Rodriguez J.R., McGowan E.B.,
RA   Esteban M.;
RT   "Expression of the firefly luciferase gene in vaccinia virus: a
RT   highly sensitive gene marker to follow virus dissemination in
RT   tissues of infected animals";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:1667-1671(1988).
XX
CC   NM (pMAMneo-LUC)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)(COS cells)
CC   CP ()
CC   FN (expression)(reporter)
CC   SE ()
CC   PA (pMAMneo)
CC   BR (pMAMneo-CAT)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMAMneo BamHI 8413bp 2423..2423, may be 5111
FT                   2. firefly BamHI-BamHI 1900bp, luciferase gene
FT                   -> pMAMneo-LUC 10339bp"
FT   misc_binding    1..1
FT                   /note="SIT EcoRI"
FT   promoter        1..91
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   misc_binding    91..91
FT                   /note="SIT HindIII"
FT   promoter        91..1543
FT                   /note="PRO mouse mammary tumor virus (MMTV)
FT                   long terminal repeat (LTR)"
FT   misc_binding    417..417
FT                   /note="SIT ClaI"
FT   misc_binding    1172..1172
FT                   /note="SIT SacI"
FT   misc_binding    6933..6933
FT                   /note="MCS NheI-SmaI-SalI-XbaI-BamHI-LUC gene-BamHI-
FT                   SmaI-KpnI-ApaI-XhoI-SalI-XhoI"
FT   CDS             0..0
FT                   /note="GEN firefly luciferase gene (luxA)"
FT   misc_feature    0..0
FT                   /note="SPL SV40 early genes"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo)"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_feature    0..0
FT                   /note="SPL SV40 splicing sequences"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   misc_binding    0..0
FT                   /note="SIT BglI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT NdeI"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
XX
SQ   Sequence 10339 BP; 2784 A; 2317 C; 2458 G; 2780 T; 0 other;
     gaattccgca ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgccatt
     tgaccattca ccacattggt gtgcacctcc aagcttgggc agaaatggtt gaactcccga
     gagtgtccta cacctagggg agaagcagcc aaggggttgt ttcccaccaa ggacgacccg
     tctgcgcaca aacggatgag cccatcagac aaagacatat tcattctctg ctgcaaactt
     ggcatagctc tgctttgcct ggggctattg ggggaagttg cggttcgtgc tcgcagggct
     ctcacccttg actcttttaa tagctcttct gtgcaagatt acaatctaaa caattcggag
     aactcgacct tcctcctgag gcaaggacca cagccaactt cctcttacaa gccgcatcga
     ttttgtcctt cagaaataga aataagaatg cttgctaaaa attatatttt taccaataag
     accaatccaa taggtagatt attagttact atgttaagaa atgaatcatt atcttttagt
     actattttta ctcaaattca gaagttagaa atgggaatag aaaatagaaa gagacgctca
     acctcaattg aagaacaggt gcaaggacta ttgaccacag gcctagaagt aaaaaaggga
     aaaaagagtg tttttgtcaa aataggagac aggtggtggc aaccagggac ttatagggga
     ccttacatct acagaccaac agatgccccc ttaccatata caggaagata tgacttaaat
     tgggataggt gggttacagt caatggctat aaagtgttat atagatccct cccttttcgt
     gaaagactcg ccagagctag acctccttgg tgtatgttgt ctcaagaaga aaaagacgac
     atgaaacaac aggtacatga ttatatttat ctaggaacag gaatgcactt ttggggaaag
     attttccata ccaaggaggg gacagtggct ggactaatag aacattattc tgcaaaaact
     catggcatga gttattatga atagccttta ttggcccaac cttgcggttc ccagggctta
     agtaagtttt tggttacaaa ctgttcttaa aacgaggatg tgagacaagt ggtttcctga
     cttggtttgg tatcaaaggt tctgatctga gctctgagtg ttctattttc ctatgttctt
     ttggaattta tccaaatctt atgtaaatgc ttatgtaaac caagatataa aagagtgctg
     attttttgag taaacttgca acagtcctaa cattcacctc ttgtgtgttt gtgtctgttc
     gccatcccgt ctccgctcgt cacttatcct tcactttcca gagggtcccc ccgcagaccc
     cggcgaccct caggtcggcc gactgcggca gctggcgccc gaacagggac cctcggataa
     gtgacccttg tctctatttc tactatttgg tgtttgtctt gtattgtctc tttcttgtct
     ggctatcatc acaagagcgg aacggactca ccatagggac caagctagcc cggggtcgac
     tctagaggat ccgagcttgg aattcctttg tgttacattc ttgaatgtcg ctcgcagtga
     cattagcatt ccggtactgt tggtaaaatg gaagacgcca aaaacataaa gaaaggcccg
     gcgccattct atcctctaga ggatggaacc gctggagagc aactgcataa ggctatgaag
     agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtgaacatc
     acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
     ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
     gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
     cgtgaattgc tcaacagtat gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag
     gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa aattattatc
     atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
     ctacctcccg gttttaatga atacgatttt gtaccagagt cctttgatcg tgacaaaaca
     attgcactga taatgaattc ctctggatct actgggttac ctaagggtgt ggcccttccg
     catagaactg cctgcgtcag attctcgcat gccagagatc ctatttttgg caatcaaatc
     attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
     acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
     ctgtttttac gatcccttca ggattacaaa attcaaagtg cgttgctagt accaacccta
     ttttcattct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc aaaacgcttc
     catcttccag ggatacgaca aggatatggg ctcactgaga ctacatcagc tattctgatt
     acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcagagagg cgaattatgt
     gtcagaggac ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
     ttcttcatag ttgaccgctt gaagtcttta attaaataca aaggatatca ggtggccccc
     gctgaattgg aatcgatatt gttacaacac cccaacatct tcgacgcggg cgtggcaggt
     cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
     ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagtc caaattgtaa
     aatgtaactg tattcagcga tgacgaaatt cttagctatt gtaatattat atgcaaattg
     atgaatggta attttgtaat tgtgggtcac tgtactattt taacgaataa taaaatcagg
     tataggtaac taaaaaggaa ttcgagctcg gtacccgggg atccccgggt accgggcccc
     ccctcgaggt cgacctcgag ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa
     cagttataat cataacatac tgttttttct tactccacac aggcatagag tgtctgctat
     taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa
     ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     aactcatcaa tgtatcttat catgtctgga tccggctgtg gaatgtgtgt cagttagggt
     gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     taggcttttg caaaaagctt cacgctgccg caagcactca gggcgcaagg gctgctaaag
     gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
     ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
     tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
     gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
     cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg
     atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     gagttcttcg cccaccccgg gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg
     ctggacgacc tcgcggagtt ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc
     agcggctatc cgcgcatcca tgcccccgaa ctgcaggagt ggggaggcac gatggccgct
     ttggtcccgg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact
     acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa
     actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga
     gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag
     tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt
     agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag
     taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata
     caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca
     taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc
     tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat
     gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg
     ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     tatcttatca tgtctggatc cccaggaagc tcctctgtgt cctcataaac cctaacctcc
     tctacttgag aggacattcc aatcataggc tgcccatcca ccctctgtgt cctcctgtta
     attaggtcac ttaacaaaaa ggaaattggg taggggtttt tcacagaccg ctttctaagg
     gtaattttaa aatatctggg aagtcccttc cactgctgtg ttccagaagt gttggtaaac
     agcccacaaa tgtcaacagc agaaacatac aagctgtcag ctttgcacaa gggcccaaca
     ccctgctcat caagaagcac tgtggttgct gtgttagtaa tgtgcaaaac aggaggcaca
     ttttccccac ctgtgtaggt tccaaaatat ctagtgtttt catttttact tggatcagga
     acccagcact ccactggata agcattatcc ttatccaaaa cagccttgtg gtcagtgttc
     atctgctgac tgtcaactgt agcatttttt ggggttacag tttgagcagg atatttggtc
     ctgtagtttg ctaacacacc ctgcagctcc aaaggttccc caccaacagc aaaaaaatga
     aaatttgacc cttgaatggg ttttccagca ccattttcat gagttttttg tgtccctgaa
     tgcaagttta acatagcagt taccccaata acctcagttt taacagtaac agcttcccac
     atcaaaatat ttccacaggt taagtcctca tttaaattag gcaaaggaat tcttgaagac
     gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     ttgcgctgct tcgcgatgta cgggccagat atacgcgtat ctgaggggac tagggtgtgt
     ttaggcgaaa agcggggctt cggttgtacg cggttaggag tcccctcagg atatagtagt
     ttcgcttttg catagggagg gggaaatgta gtcttatgca atacacttgt agtcttgcaa
     catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     atggattgga cgaaccact
//