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ID   PMB124     preliminary; circular DNA; SYN; 2725 BP.
XX
AC   IG5013;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMB124 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pMB121, pMB122 from pNIMB
RC   pMB123 from pMB121
RC   pMB124 from pMB122
RA   Siniakov A.N., Serpinsky O.I., Daniliuk N.K., Chizhikov V.E.,
RA   Degtiarev S.Kh.;
RT   "[Plasmids pMB123 and pMB124--vectors for obtaining subfragments of
RT   DNA with random sticky ends]";
RL   Bioorg. Khim. 15:638-647(1989).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   MCS, oligonucelotide linker.
CC   NM (pMB124)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pMB122 from pNIMB from pUR222)
CC   BR (pMB123 from pMB121 from pNIMB from pUR222)
CC   OF (pIL581)(pIL912)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pNIMB remove EcoRI-BamHI 12bp,
FT                   \ pUR222 1830..oligo/2703bp
FT                   2. oligo 10bp ggatgacgcg
FT                   3. oligo 18bp tcgacgcgtcatcctgca
FT                   -> pMB121 2719bp
FT                   1. pMB121 remove SalI-PstI 10bp,
FT                   \ pUR222 1845..1855/2709bp
FT                   2. oligo 16bp gatccgcgtcatccag
FT                   3. oligo 16bp aattctggatgacgcg
FT                   -> pMB124 2741bp"
FT   -               1..1829
FT                   /note="pUR222 1..1829 1829bp
FT                   EcoRI = G^AATTC
FT                   \         aattc..."
FT   -               1830..1845
FT                   /note="aattctggatgacgcg 16bp
FT                   \ ...gacgcg
FT                   BamHI =   G^GATCC
FT                   \           gatcc..."
FT   -               1846..1861
FT                   /note="gatccaagctagcttg 16bp
FT                   \ ...cctg
FT                   SalI =  G^TCGAC
FT                   \         tcgacg..."
FT   -               1862..1879
FT                   /note="tcgacgcgtcatcctgca 18bp
FT                   \  ...cctgca
FT                   PstI = CTGCA^G"
FT   -               1880..2725
FT                   /note="pUR222 1855..2700 846bp"
FT   promoter        0..0
FT                   /note="E. coli lacZ PO"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
FT   misc_binding    0..0
FT                   /note="MCS PstI-FokI-HgaI-AccI/HindII/SalI-
FT                   BamHI-HgaI-FokI-EcoRI"
XX
SQ   Sequence 2725 BP; 662 A; 671 C; 707 G; 685 T; 0 other;
     tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgatgccg
     ggcaagagca actcggtcgc gcgatacact attctcagaa tgacttggtt gagtactcac
     cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatcccta
     acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     gaactctgta gcaccgccta catacctcgt cctgctaatc ctgttaccag tggctgctgc
     cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gccattcgcc
     attcaggcta cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     gctggcgaag gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     gtcacgacgt tgtaaaacga cggccagtga attctggatg acgcggatcc aagctagctt
     gtcgacgcgt catcctgcag caattcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     ggtgcctaat gagtgagcta agtcacatta attgcgttgc gctcactgcc cgctttccag
     tcgggaaacc tgtcgtgcca gctggattaa tgaatcggcc aacgcgccgg gagaggcggt
     ttgcgtattg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtaatac
     actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     gacgcgccct gatgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     cagttgggtg cacgagtggg ttaca
//