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ID PMB9 preliminary; circular DNA; SYN; 5295 BP.
XX
AC M13468; IG1088; ATCC37019;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pMB9 - complete, kil gene.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-138
RC pEAP1R from pEAP1
RC pEAP2R from pEAP2
RC pEAP3 from pEAP1
RC pEAP4, pEAP4R from pEAP3 & pMB9
RC pEAP6B from pEAP3 & linker
RC pACAP2 from pEAP2 & pACYC184
RC pACAP2R from pEAP2R & pACYC184
RA Kobayashi T., Kato C., Kudo T., Horikoshi K.;
RT "Excretion of the penicillinase of an alkalophilic Bacillus sp.
RT through the Escherichia coli outer membrane is caused by
RT insertional activation of the kil gene in plasmid pMB9";
RL J. Bacteriol. 166:728-732(1986).
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RN [2]
RC pEAP1, pEAP2 from pMB9 & Bacillus penicillinase gene
RA Kudo T., Kato C., Horikoshi K.;
RT "Excretion of the penicillinase of an alkalophilic Bacillus sp.
RT through the Escherichia coli outer membrane";
RL J. Bacteriol. 156:949-951(1983).
XX
RN [3]
RC pMB9 from pMB8 & pSC101
RC pBR312, pBR26 from pMB9 & pSF2124, TnA/Tn3 amp gene
RC pBR313, pBR315, pBR316 from pBR312
RC pBR317, pBR318 from pBR313
RA Bolivar F., Rodriguez R.L., Betlach M.C., Boyer H.W.;
RT "Construction and characterization of new cloning vehicles, I.
RT ampicillin-resistant derivatives of the plasmid pMB9";
RL Gene 2:75-93(1977).
XX
RN [4]
RC [pMB1 from ColE1]
RC pMB3 from pMB1 & Tn3, amp gene
RC pMB8 from pMB3
RC pMB9 from pMB8 & pSC101, tet gene
RC pBR312 from pMB9 & pSF2124, Tn3
RC pBR313 from pBR312
RC pBR318, pBR320 from pBR313
RC pBR322 from pBR318 & pBR320
RA Rodriguez R.L., Tait R.C.;
RT ;
RL Recombinant DNA Technology An Introduction 0:187-187(1983).
RL Rodriguez R.L., Tait R.C., Addison-Wesley, Reading MA.
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CC Created by Moore, July 1995, under contract with NCBI.
CC ATCC size is 5200 bp.
CC Medium is 1273 LB plus tetracycline.
CC NCBI gi: 207849
CC NM (pMB9)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Sigma)(ATCC)
CC HO (E.coli RRI)(E.coli HB101)(E.coli C600)(E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (colicin E1)(Tn3)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. ColE1 6646bp
FT -> pMB1 2000bp
FT 1. pMB1 2000bp
FT 2. Tn3 1800bp, amp gene
FT -> pMB3 3800bp
FT 1. pMB3 3800bp
FT \ atgaggaaaagattttttgtgggaatattcgcgataaacctccttgttgg
FT \ atgtcaggctaactatatacgtgatgttcagggagggaccatcgcaccat
FT \ cctcctcttctaaactgacggggatcgcggttcagtag
FT -> pMB8 3847bp [similar to pVH51]
FT 1. pMB8/pVH51 EcoRI 3847bp 3846..3846, ori/cat gene
FT 2. pSC101 EcoRI-BalI 1448bp 9262..9263..1447, tet gene
FT -> pMB9 5295bp [unique EcoRI, HindIII, SalI, BamHI]"
FT - 1..3845
FT /note="pVH51 1..3845 3845bp
FT EcoRI = G^AATTC"
FT - 3846..5293
FT /note="pSC101 9262..9263..1446 1448bp
FT BalI = TGG^CCA
FT EcoRI = G^AATTC"
FT - 5294..5295
FT /note="pVH51 3846..3847 2bp"
FT CDS 0..0
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT misc_binding 0..0
FT /note="SIT HindIII"
FT misc_binding 0..0
FT /note="SIT SalI"
FT mRNA 0..0
FT /note="GEN E. coli imm mRNA [4],[8]"
FT CDS 0..0
FT /note="GEN E. coli colicin E1 immunity protein (imm)"
FT misc_binding 0..0
FT /note="SIT EcoRI"
FT CDS 1..138
FT /note="GEN E. coli kil gene; NCBI gi: 207850."
FT misc_RNA 0..0
FT /note="GEN E. coli RNA II"
FT misc_RNA 0..0
FT /note="GEN E. coli RNA I (3' end +/- 1 bp) [5]"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)
FT (+/- 1 bp) [1]"
FT mRNA 0..0
FT /note="GEN E. coli mob mRNA (5' end putative) [4],[8]"
FT misc_feature 0..0
FT /note="SIT E. coli relaxation (nic) cut site [2]"
FT CDS 0..0
FT /note="GEN E. coli mob1 protein; (GTG start codon)"
FT CDS 0..0
FT /note="GEN E. coli RNA I inhibition modulator protein;
FT (rom - GTG start codon)"
FT mRNA 0..0
FT /note="GEN E. coli exc mRNA [4],[8]"
FT CDS 0..0
FT /note="GEN E. coli entry exclusion protein 2 (exc2)"
FT CDS 0..0
FT /note="GEN E. coli entry exclusion protein 1 (exc1)"
FT mRNA 0..0
FT /note="GEN E. coli colE1-kil mRNA [8]"
FT CDS 0..0
FT /note="GEN E. coli colicin E1 protein (cea)"
XX
SQ Sequence 5295 BP; 1237 A; 1305 C; 1510 G; 1243 T; 0 other;
ttctatgctc ctatattgat aagaataaac ttaatactat aaatgaggtg ttagggattt
aattattctt tattgatata aaaagtccta gcaatccaaa tgggattgct aggaccaaac
aaagtagatt atatagcata aataggttta attttgctac gggggcgtta tttaggtttt
ttcttctttc gaaaaaatct ttctttatga agttaaaagc tatgtattca atagcatatt
ttgaatatgg acatagaata gtgcttatca ctattgcata tagcatctta tctgacacaa
ggaaataata cccttcgctg ttttttgtta taaggtatat atatataagt gtgcagtaca
ggccaaataa aatatttttt atgtagtatc ttaagctcat aaattaaacc tcgccatata
ttcttttcat tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgcg
ataaacctcc ttgttggatg tcaggctaac tatatacctg atgttcaggg agggaccatc
gcaccatcct cctcttctaa actgacgggg atcgcggttc agtagaaaag attaaaggat
cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
taccaacggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag tcgggccact
acttcaagaa ctctgtagca ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt
ggctgctgcc agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc
ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc
cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
gagggagctt ccagggggaa acgcctggta gctttatagt cctgtcgggt ttcgccacct
ctgacttgag cgtctatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
ctgctacgtg gccttcttcc tgttcctggt cttttgctca catgttcttt ccggccttat
cccctgattc tgtggataac tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca
gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc
attttctcct tacgcatctg tgcggcattt cacacccggc atggcgtact tttcatacaa
tccgcactga tgccgcatgg ttaagccagt atacactccg ctatcgctac gtgactgggt
cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtcagct
cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg
aagcgattca caaatatcgg cctgttcatc tgcgtccagt tcgttgagct tctccagcag
cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttagt
cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc gatgaaacgc
gagaggatgc tcacaatacg ggttactgat gatgaacatg cccggttact tgaacgctgt
gagggtaaac aactggcggt atggatgcgg cgggtctgcc tgggggagcc ggttgcccgt
tccggaaaac tgccgacact ggcaccgccg ttactgcgtc agctggccgc catcggaaat
aacctgaatc agacagcccg taaggtgaac agcgggcagt ggtcttccgg tgaccgggtt
caggtggtgg ccgcactgat ggccatcggg gatgagctgc gccggctgcg tctggctgtc
agggaacagg gggcgcggga tgatagttaa atttcatgcc aggggaaaag gtggtggcag
tggtccggtt gattacctgc tggggaggga gcgtaaccgc gaaggcgcaa cggtgcttca
gggtataccg gaagaagtcc gggaactcat cgatgccacg ccatttgcga agaaatacac
gtccggtgtt ctgtcgttcg cggagaagga gctgccgccg ggaggacgtg aaaaagtgat
ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg
ggtggagcac caggacaagg gacggcttga gctgaatttt gtcattccga acatggagct
acagaccgga aaacgcctcc agccgtacta cgaccgcgca gacaggccta gaattgatgc
ttggcagacg ctgttaaatc accattacgg gctgcatgac ccgaacgccc cggagaaccg
caggacgctg acactccctg ataacctgcc tgaaacgaaa caggcgcttg ctgagggcgt
cacgcgaggt atagatgcac tttaccatgc cggagagata aaaggccgtc aggatgtgat
tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg acgcgaagca gtatcagcat
tgcagatccg aacggcggga agaatatcag gctgaaagga gcattttatg agcaatcttt
tgcagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa
tgctgaacaa cgagttcagg aggctcggcg aatctgtaag cgaggctgtg acatcaaacg
agacgaaaat cagagacgct atagccctgt tcacagcctc gacagaggaa tcgctggaaa
aacaccggga aggggtgaaa gaggcgatga tgcagcacag gagggacgtg ttaaagctgg
cagggaatac gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg
ggacgctctg gtatcttgga gggaggatac aggcgaacct ggaagaaatc aggaagcagg
aagagacatt gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga
acaggaaatt ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga
aagagtgggt aatggaactt ggatgaagaa agagtgaaca tgatggttcc gccatggggc
gggcggtcat ggagttgtca ctggcagatt tacctatgac ccagcaaaac atcatcgaca
agctggaacg gtaccggaag gaaacgggaa acgtgatagg taagggtgtg aacgggatgc
agctgagata gtcaggaagg gtagtaaagc tgtgaagtag gcacaaaaaa cccggcgcgg
tggccggttg ttaaagctca atatcaacta tgaaaagttt actaatagcc ccttttctaa
ctgctgcttt ggcagttact tttaccgctg actcagtact tagacccgtt gacagaaaac
gactaagtga aatgagatac gggttgtctg cctttgaagc agatggatcg cttatttgtg
ctgagatgcg cttatctgaa tcatcacctt ctataatgac tttagcggtc attcggtgag
cgtcgaattc tcatgtttga cagcttatca tcgataagct ttaatgcggt agtttatcac
agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca
tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg gtactgccgg
gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc gtgctgctag
cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg tccgaccgct
ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac tacgcgatca
tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc ggcatcaccg
gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc
tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg
ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg
gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc
tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg
acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca
ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg
catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat
ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg gattcaccac
tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg
gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tctcgggcag
cgttgggtcc tggaa
//