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ID   PMBC2T     preliminary; circular DNA; SYN; 3123 BP.
XX
AC   X77750;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pMBC-2T - complete.
XX
KW   cloning vector; eukaryote expression plasmid.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-3123
RC   pMBC-2T from pBR322
RA   Morgan G.T., McMahon H.;
RT   "Alterations in cloned Xenopus ribosomal spacers generated by
RT   high-frequency plasmid recombination";
RL   Gene 49:389-394(1986).
XX
RN   [2]
RP   1-3123
RC   pMBC-2T from pBR322
RA   Schaper F.;
RT   ;
RL   Submitted (14-FEB-1994) to EMBL/GenBank/DDBJ by:
RL   Schaper F., Gesellschaft fr. Biotechnologische Fors., (GBF),
RL   Mascheroder Weg 1, 38106 Braunschweig, FRG.
XX
CC   NCBI gi: 456203
CC   NM (pMBC-2T)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   -> pMBC-2T 3123bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        6..455
FT                   /note="PRO from MT7"
FT   intron          463..521
FT                   /note="SV40 19S mRNA, from VP1"
FT   misc_feature    529..591
FT                   /note="MCS pUC8"
FT   polyA_signal    626..862
FT                   /note="PLA SV40, from VP1"
FT   terminator      1022..1157
FT                   /note="TER T7"
XX
SQ   Sequence 3123 BP; 815 A; 753 C; 761 G; 794 T; 0 other;
     tcgataatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
     aaggcatggg aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga
     gaaacaggag aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccgctcag
     ggccaagaac agttggaaca ggagaattgg gccaaacagg atatctgtgg taagcagttc
     ctgccccgct cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt
     ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
     tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
     caataaaagc gataatacga ctcactatag ggcgcctcga ggaactggaa aaccagaaag
     ttaactggta agtttagtct ttttgtcttt tatttcaggt cccggatcga attgcggccg
     cgaattcgag ctcgcccggg gatcctctag agtgacctgc agcccaagct tggtacctcc
     ctttagtgag ggttaattga gcttcgatcc agacatgata agatacattg atgagtttgg
     acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     caaatgtggt atggctgatt atgatcctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     aggaactata tccggatcag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     ggcatcagag cagattgtac tgagagtgca ccatatgtcg ggccgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtg cgcaacgttg ttgccattgc tacaggcatc
     gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     acgaggccct ttcgtcttca agaattggtc gatcgaccaa ttctcatgtt tgacagctta
     tca
//