back Return to this vector's summary.
ID   PMBL1      preliminary; circular DNA; SYN; 5208 BP.
XX
AC   L08933; VB0129;
XX
DT   04-JUN-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pMBL1 (pOS185?) - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5208
RC   pMBL1 or pOS185
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
XX
RN   [2]
RC   pMBL1 or pOS185
RA   Enger-Valk B.E.;
RT   ;
RL   Submitted (21-JAN-1982) on floppy disc by:
RL   Enger-Valk B.E., .
XX
CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   Revised according to sequence of pBR322 by F. Pfeiffer
CC   NM (pMBL1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(EcoTrp)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. E. coli, trp promoter
FT                   -> pMBL1 5208bp"
FT   misc_feature    1..33
FT                   /note="from pBR322"
FT   misc_feature    34..2274
FT                   /note="from EcoTrp"
FT   misc_feature    2275..5208
FT                   /note="from pBR322"
FT   CDS             complement(4143..4931)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 5208 BP; 1283 A; 1360 C; 1303 G; 1262 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctagctttt gtcagtgcgc aaaaagatcc
     tgaatttcag gctcagttca acgacctgct gaaaaactat gccgggcgtc caaccgcgct
     gaccaaatgc cagaacatta cagccgggac gaacaccacg ctgtatctca agcgtgaaga
     tttgctgcac ggcggcgcgc ataaaactaa ccaggtgctg gggcaggcgt tgctggcgaa
     gcggatgggt aaaaccgaaa tcatcgccga aaccggtgcc ggtcagcatg gcgtggcgtc
     ggccctggcc agcgccctgc tcggcctgaa atgccgtatt tatatgggtg ccaaagacgt
     tgaacgccag tcgcctaacg tttttcgtat gcgcttaatg ggtgcggaag tgatcccggt
     gcatagcggt tccgcgacgc tgaaagatgc ctgtaacgag gcgctgcgcg actggtccgg
     tagttacgaa accgcgcact atatgctggg caccgcagct ggcccgcatc cttatccgac
     cattgtgcgt gagtttcagc ggatgattgg cgaagaaacc aaagcgcaga ttctggaaag
     agaaggtcgc ctgccggatg ccgttatcgc ctgtgttggc ggcggttcga atgccatcgg
     catgtttgct gatttcatca atgaaaccaa cgtcggcctg attggtgtgg agccaggtgg
     tcacggtatc gaaactggcg agcacggcgc accgctaaaa catggtcgcg tgggtatcta
     tttcggtatg aaagcgccga tgatgcaaac cgaagacggg cagattgaag aatcttactc
     catctccgcc ggactggatt tcccgtctgt cggcccacaa cacgcgtatc ttaacagcac
     tggacccgct gattacgtgt ctattaccga tgatgaagcc cttgaagcct tcaaaacgct
     gtgcctgcac gaagggatca tcccggcgct ggaatcctcc cacgccttgg cccatgcgtt
     gaaaatgatg cgcgaaaacc cggataaaga gcagctactg gtcgttaacc tttccggtcg
     cggcgataaa gacatcttca ccgttcacga tattttgaaa gcacgagggg aaatctgatg
     gaacgctacg aatctctgtt tgcccagttg aaggagcgca aagaaggcgc attcgttcct
     ttcgtcacgc tcggtgatcc gggcattgag cagtcattga aaattatcga tacgctaatt
     gaagccggtg ctgacgcgct ggagttaggt atccccttct ccgacccact ggcggatggc
     ccgacgattc aaaacgccac tctgcgcgcc tttgcggcag gtgtgactcc ggcacaatgt
     tttgaaatgc tggcactgat tcgccagaaa cacccgacca ttcccattgg cctgttgatg
     tatgccaatc tggtgtttaa caaaggcatt gatgagtttt atgcccagtg cgaaaaagtc
     ggcgtcgatt cggtgctggt tgccgatgtg ccagttgaag agtccgcgcc cttccgccag
     gccgcgttgc gtcacaacgt cgcacctatc ttcatctgcc cgccaaatgc cgatgacgac
     ctgctgcgcc agatagcctc ttacggtcgt ggttacacct atttgctgtc acgagcaggc
     gtgaccggcg cagaaaaccg cgccgcgtta cccctcaatc atctggttgc gaagctgaaa
     gagtacaacg ctgcacctcc attgcaggga tttggtattt ccgccccgga tcaggtaaaa
     gcagcgattg atgcaggagc tgcgggcgcg atttctggtt cggccattgt taaaatcatc
     gagcaacata ttaatgagcc agagaaaatg ctggcggcac tgaaagtttt tgtacaaccg
     atgaaagcgg cgacgcgcag ttaatcccac agccgccagt tccgctggcg gcattttaac
     tttctttaat gaagccggaa aaatcctaaa ttcatttaat atttatcttt ttaccgtttc
     gcttaccccg gtcgatcgtg gacttacgtc atttttccgc ccaacagtaa tataaacaaa
     caaattaaac ccgcaacata acaccagtaa aatcaataat tttctctaag tcacttattc
     ctcaggtaat tcttaatata tccagaatgt tcctcaaaat atattttccc tctatcttct
     cgttgcgctt aatttgacta attctcatta gcgactaatt ttaatgagtg tcgagcagcg
     ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct
     ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg
     actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg
     tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca
     tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc
     attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct
     cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct
     ctcgtttcat cggtatcatt acccccatga acagaaattc ccccttacac ggaggcatca
     agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt
     aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc
     gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg
     tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaa
//